Abstract:This paper presents sample stacking with in-column silylation (SIS) for quantitative analysis of less volatile polar compounds using gas chromatography-mass spectrometry (GC-MS). This was achieved by the combination of sandwiched in-column silylation and multiple injections (up to 100 times or 100 μL in total). N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) was used as a silylating reagent. For the SIS technique, samples were introduced multiple N times (N = 2~100) into a capillary column in between BSTFA i… Show more
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