Increasing the instrumental throughput of gas chromatography method using multiple injections in a single experimental run: Application in determination of friedelan-3-ol and friedelin in Maytenus ilicifolia

Vistuba, Jacqueline Pereira; Piovezan, Marcel; Pizzolatti, Moacir Geraldo; Rebelo, Andrey Martinez; Azevedo, Mônia Stremel; Vitali, Luciano; Costa, Ana Carolina Oliveira; Micke, Gustavo Amadeu

doi:10.1016/j.chroma.2012.11.087

Cited by 13 publications

(10 citation statements)

References 24 publications

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“…Note the presence of impurities early in the chromatogram and the impurity directly following the second enantiomer. Estimation of the number of injections possible can be calculated by the following equation reported by Micke et al

n_{inj} = \frac{{tR}_{italicA} - {tR}_{interf 1}}{({tR}_{italicimp} - {tR}_{italicA}) + (\frac{w_{A} + w_{imp} + w_{italicinterf italic1}}{3})}

where tR A , tR interf1 , and tR imp are the retention times of the first analyte and the last analyte and the last interfering peaks before the peak of the first analytes and after the peak of the last analyte, respectively; w A , w B , w interf1 , and w imp are the peak widths of the respective peaks.…”

Section: Resultsmentioning

confidence: 99%

Section: Case 2: Stacked Injection Mode (Gc-fid)mentioning

confidence: 99%

“…Recently, Vistuba et al. reported the use of MISER GC stacking injections with FID detection for the determination of friedelan. Here, we investigate the use of a stacked injection application for chiral GC, where long run times are often necessary to resolve the analytes of interest, and where no analyte‐specific detector for regio‐ or stereoisomers with the same mass exists.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Multiple‐injection high‐throughput gas chromatography analysis

Schafer

Wang

Welch

2016

J of Separation Science

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Multiple-injection techniques have been shown to be a simple way to perform high-throughput analysis where the entire experiment resides in a single chromatogram, simplifying the data analysis and interpretation. In this study, multiple-injection techniques are applied to gas chromatography with flame ionization detection and mass detection to significantly increase sample throughput. The unique issues of implementing a traditional "Fast" injection mode of multiple-injection techniques with gas chromatography and mass spectrometry are discussed. Stacked injections are also discussed as means to increase the throughput of longer methods where mass detection is unable to distinguish between analytes of the same mass and longer retentions are required to resolve components of interest. Multiple-injection techniques are shown to increase instrument throughput by up to 70% and to simplify data analysis, allowing hits in multiple parallel experiments to be identified easily.

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n_{inj} = \frac{{tR}_{italicA} - {tR}_{interf 1}}{({tR}_{italicimp} - {tR}_{italicA}) + (\frac{w_{A} + w_{imp} + w_{italicinterf italic1}}{3})}

Section: Resultsmentioning

confidence: 99%

Section: Case 2: Stacked Injection Mode (Gc-fid)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Multiple‐injection high‐throughput gas chromatography analysis

Schafer

Wang

Welch

2016

J of Separation Science

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“…Among the various substances isolated and identified from the roots, leaves and branches in the studies carried out with M. ilicifolia, the ones that stand out the most are terpenes (isotenginone II and trigenone), triterpenes (maytenoic acid, friedenalol, friedelin and maytene), dimeric triterpenes (congorosins A and B, friedenelol), tannins (among these the gallic ones, epigallocatechin gallate, epicatechin and epigallocatechin, are the ones that stand out the most), glycolipids (monogalactosildiacilglicerol, digalactosildiacilglicerol and tetragalyglycerols mayine) and maytansine, [7,10,[12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Compounds in the Plant Maytenus ilicifolia Mart. Ex Reissek during the Initial and Adult Development Stage

Alvarenga

Coelho

Sakai

et al. 2020

EJMP

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Maytenus ilicifolia Mart. Ex Reissek, was first studied in the 60s, but only in the 80s and 90s were the studies were driven by scientific research that attributed medicinal properties to the plant, such as healing actions for human gastric diseases. In this work, a study was carried out on the characterization of the chemical composition of M. ilicifolia at different stages of development, using spectroscopic techniques of sustainable character, which do not require toxic solvents as previous preparations. Extracts from cotyledons, seedlings that have never been researched in the literature and leaf/stem were lyophilized to characterize the compounds present in this plant using photoacoustic and spectroscopy techniques in the Infrared spectroscopy using Fourier Transform-FTIR with ATR. The photoacoustic results show that there is a differentiation of development stages in the plant due to the greater presence of chlorophylls and terpenes in the seedlings and their absence in the cotyledon, which is characterized as the reserve part in the germination phase of the plant. The range of bands the literature reports for epigallocatechin and friedelin, the possible agents responsible for the medicinal effects of the plant were mainly seen in the cotyledon, thus demonstrating the use of the seedling in its initial stage, to extract and obtain these active components.

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“…developed multiple injections in a single experimental run chromatography, where in analogy to the FIA technique, rapid sequential analysis of samples is carried out in a single run using HPLC or HPLC–MS . This approach has successfully been applied several times as a screening platform in liquid chromatography and GC .…”

Section: Introductionmentioning

confidence: 99%

Implementation of Hadamard encoding for rapid multisample analysis in liquid chromatography

Siegle

Trapp

2015

J. Sep. Science

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Multiplexing based on pseudo-binary modulation sequences is known to increase the signal-to-noise ratio. In this work, Hadamard transform multiplexing is used in high-performance liquid chromatography to increase the sample throughput. Using structured modulation sequences to encode and control sample injections in combination with a fitting algorithm to deconvolute the complex data allowed us to evaluate convoluted chromatograms of up to 128 samples containing three and five analytes, respectively, with good accuracy (<2% deviation). In comparison to conventional high-performance liquid chromatography the analysis time could be reduced by 30 and 55%, respectively.

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Increasing the instrumental throughput of gas chromatography method using multiple injections in a single experimental run: Application in determination of friedelan-3-ol and friedelin in Maytenus ilicifolia

Cited by 13 publications

References 24 publications

Multiple‐injection high‐throughput gas chromatography analysis

Multiple‐injection high‐throughput gas chromatography analysis

Characterization of Compounds in the Plant Maytenus ilicifolia Mart. Ex Reissek during the Initial and Adult Development Stage

Implementation of Hadamard encoding for rapid multisample analysis in liquid chromatography

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