2014
DOI: 10.1186/s12896-014-0097-5
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Abstract: BackgroundThe enzyme cellobiose dehydrogenase (CDH) can be used to oxidize lactose to lactobionic acid. As Sclerotium rolfsii is known to be a good producer of CDH, the aim of this paper was to simplify its production and secondly to systematically study its purification aiming for a high yield. Two preservation methods (freezing and freeze-drying) and the influence of several protectants were investigated.ResultsProduction of cellobiose dehydrogenase was optimized leading to a more simplified medium compositi… Show more

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Cited by 5 publications
(2 citation statements)
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“…Therefore, it is relevant to maintain the binary structure of the enzyme (Bollella et al 2018 ). The effective stabilization of CDH only with sugars as cryoprotectants during freezing and freeze-drying (Fischer et al 2014 ) has been investigated, but no studies are available on the CDH storage protection effect with polysaccharides.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Therefore, it is relevant to maintain the binary structure of the enzyme (Bollella et al 2018 ). The effective stabilization of CDH only with sugars as cryoprotectants during freezing and freeze-drying (Fischer et al 2014 ) has been investigated, but no studies are available on the CDH storage protection effect with polysaccharides.…”
Section: Resultsmentioning
confidence: 99%
“…Application of CDH in all proposed areas requires high stability of the enzyme and, in particular, maintenance of its intact form with a cytochrome domain. A review of previous literature focused on the stabilization of CDH shows only reports of the use of sugars as effective protective substances in the freeze-drying process (Fischer et al 2014 ) and stabilization of the enzyme with genetic engineering techniques (Balaž et al 2019 ). There are no available research regarding the possibilities of the use of naturally produced fungal and bacterial EPSs as protective agents and modulators of CDH catalytic activity.…”
Section: Introductionmentioning
confidence: 99%