Current perspective on improved fermentative production and purification of fungal cellulases for successful biorefinery applications: a brief review

Dey, Pinaki; Rangarajan, Vivek; Singh, Joginder; Nayak, Jayato; Dilip, Kevin Joseph

doi:10.1007/s13399-020-01227-6

Cited by 14 publications

(10 citation statements)

References 209 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This pretreatment promotes lignin separation and hemicellulose hydrolysis, and reduces cellulose crystallinity, to expose the maximum cellulose content to microorganisms [54]. In order to make the acid pretreatment more efficient [31], the water hyacinth biomass was ground to reach fine particle sizes (from 0.420 to 0.841 mm).…”

Section: Cellulase and Xylanase Production Of P Crustosum Abq1 By Sol...mentioning

confidence: 99%

“…Cellulases and xylanases share several industrial applications, such as the production of paper and pulp, textiles, juice clarification and extraction, food additives, beer manufacturing, animal feed production, and bioethanol production [29,[31][32][33][34]. For industrial applications, cellulases and xylanases are essential in large quantities [29,34,35].…”

Section: Introductionmentioning

confidence: 99%

“…For industrial applications, cellulases and xylanases are essential in large quantities [29,34,35]. Although cellulases in particular play a crucial role in the hydrolysis of lignocellulosic materials, the cost of these enzymes remains the main obstacle to the development of large-scale lignocellulosic biorefineries for commercial purposes [31]. To reduce the costs of enzymes, the following aspects should be considered: (i) selection of a potent producer microorganism; (ii) improvement in the production and productivity by optimizing culture conditions (including media components) [29]; (iii) use of cheaper feedstocks (including agricultural residues and weeds) as the main carbon sources and inducers for microbial culture [36]; and (iv) the use of solid-state fermentation (SSF) as a cost-effective fermentation process that can achieve higher cellulase production compared to submerged fermentations [30,37].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cellulase and Xylanase Production by a Newly Isolated Penicillium crustosum Strain under Solid-State Fermentation, Using Water Hyacinth Biomass as Support, Substrate, and Inducer

Espinoza-Abundis,

Soltero-Sánchez,

Romero-Borbón

et al. 2023

Fermentation

View full text Add to dashboard Cite

Cellulase and xylanase have been widely studied for bioconversion processes and applied in various industries. The high cost of these enzymes remains to be the major bottleneck for large-scale commercial application of lignocellulosic biorefinery. The use of agroindustrial residues and weeds as fermentation substrates is an important strategy to increase cellulolytic enzymes production and reduce costs. Penicillium crustosum was newly isolated and selected to study its enzyme production during solid-state fermentation (SSF). Natural and pretreated water hyacinth (WH) biomass was used as support, substrate and inducer of cellulases and xylanases. Thermochemical pretreatments of WH biomass at 121 °C and sulfuric acid at three concentrations (0.2, 0.6 and 1 M) were assayed. The pretreatments of WH biomass released mono- and oligo-saccharides that favored fungal growth and enzymes production on SSF. WH is a cost-effective substrate-support and inducer, which to be used as a solid medium, was impregnated with a saline solution, containing only (NH4)2SO4, KH2PO4 y MgCl2. Maximum cellulases (carboxymethylcellulase (CMCase)) and xylanases productions of P. crustosum cultured on SSF were reached using the WH pretreated biomass with H2SO4 0.6 M and 121 °C. The simultaneous CMCase and xylanases production reached (647.51 and 4257.35 U/g dry WH, respectively) are among the highest values ever reported.

show abstract

Section: Cellulase and Xylanase Production Of P Crustosum Abq1 By Sol...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cellulase and Xylanase Production by a Newly Isolated Penicillium crustosum Strain under Solid-State Fermentation, Using Water Hyacinth Biomass as Support, Substrate, and Inducer

Espinoza-Abundis,

Soltero-Sánchez,

Romero-Borbón

et al. 2023

Fermentation

View full text Add to dashboard Cite

show abstract

“…Cellulolytic enzymes play the most indispensable and centralized role in the hydrolysis of cellulosic material. Effective enzymatic hydrolysis is one of the major prerequisites in the development of a successful lignocellulosic bio refinery (Dey et al 2021).…”

Section: Introductionmentioning

confidence: 99%

Lignocellulosic Ezymes of Basidial Fungi-Isolated from Different Ecological Niches of Georgia

TSIKLAURI

JOBAVA

KHVEDELIDZE

et al. 2021

EPSTEM

View full text Add to dashboard Cite

Great interest in basidiomycetes for targeted technological treatment of agro-industrial plant substrates are conditioned by their ability to produce lignocellulosic enzymes. Consisting of 29 soil-climatic zones, Georgia (South Caucasus area) represents a unique place for the isolation of microorganisms. In order to study the biochemistry and physiology of wood-degrading basidiomycetes, samples of wood-degrading basidiomycetes were collected from different taxonomic niches of Georgia. 45 strains were obtained as pure cultures and 32 ones – identified. Producers of lignocellulosic enzymes were revealed among test fungi under solid-state and submerged cultivation conditions. Pleurous ostreatus GV10, Pleurotus drynus IN 11 and Fomes fomentarius GK33 were found to be the best producers of cellulosic enzymes using orange and mandarin peels, wheat and Potato straw, wheat bran as substrates, and Ganoderma lucidum GB03 – the best producer of laccase during cultivation on orange and mandarin peels waste. The influence of lignocellulose on the accumulation of the enzymes laccase, xylanase and Filter paper assay was studied.

show abstract

“…In the mentioned processes, the role of white rot basidial fungi capable of synthesizing hydrolyzing and oxidizing enzymes, which play an important role in degradation of polysaccharides and lignin of plant biomass, should be especially noted (Huang et al, 2008). Effective enzymatic hydrolysis is one of the major prerequisites in the development of a successful lignocellulosic biorefinery (Dey et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Conversion of agro-industrial wastes into non-toxic protein-rich food additives by basidial fungi strains isolated from diverse ecosystems of Georgia

Kvesitadze¹,

Tsiklauri²,

Khvedelidze³

et al.

Global NEST International Conference on Environmental Science &Amp; Technology

View full text Add to dashboard Cite

Great interest to basidial fungi is determined by their potential to convert number of lignocellulosic wastes into nontoxic compounds, cheap food, pharmaceuticals, etc. The majority of basidial fungi strains are characterized by high activity of enzymes degrading plant biopolymers, including lignin, which attracts special attention because of their use in different industries and medicine. Among 53 tested basidial fungi strains best results were obtained by Pleurotus drynus IN11 and Ganoderma sp. GV02 strains while growing on orange wastes, where 20% and 27% of protein, respectively, and up to 50% of carbohydrates were accumulated. After 10 - 20 days of solid state fermentation (SSF) in the same biomass the content of lignin was detected only in trace amounts. The content of microelements was (mg/kg): calcium 1200 - 6200, potassium 2100 - 2800, and sodium 90 - 708. Further investigation of strains allowed selection of active producers of soluble polysaccharides among which Pleurotus ostreotus and the representatives of genus Ganoderma sp. (10 - 14 g/L) were the most active. During the submerged fermentation (SF) the highest potential to produce extracellular antioxidants were detected in several strains of Ganoderma sp. (up to 6 g/L). Some selected Ganoderma strains expressed very high laccase extracellular activity (about 125000 U/L).

show abstract

Current perspective on improved fermentative production and purification of fungal cellulases for successful biorefinery applications: a brief review

Cited by 14 publications

References 209 publications

Cellulase and Xylanase Production by a Newly Isolated Penicillium crustosum Strain under Solid-State Fermentation, Using Water Hyacinth Biomass as Support, Substrate, and Inducer

Cellulase and Xylanase Production by a Newly Isolated Penicillium crustosum Strain under Solid-State Fermentation, Using Water Hyacinth Biomass as Support, Substrate, and Inducer

Lignocellulosic Ezymes of Basidial Fungi-Isolated from Different Ecological Niches of Georgia

Conversion of agro-industrial wastes into non-toxic protein-rich food additives by basidial fungi strains isolated from diverse ecosystems of Georgia

Contact Info

Product

Resources

About