Optimization of Process Variables for Extracellular Lipase Production from Emericella nidulans NFCCI 3643 Isolated from Palm Oil Mill Effluent (POME) Dump Sites Using OFAT Method

Lanka, Suseela; Pydipalli, Muralidhra; Latha, J. Naveena Lavanya

doi:10.3923/jm.2015.38.53

Cited by 7 publications

(1 citation statement)

References 28 publications

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“…Furthermore, the presence of surfactants increases cell permeability, allowing for the export of a variety of molecules across the cell membrane, including protein secretion. Surfactants also make it easier for the enzyme to make contact with the substrate [19]. Hence, this may be the principle behind the enhancement of lipase production by surfactants.…”

Section: Discussionmentioning

confidence: 99%

Enhancing Lipase Production of Bacillus salmalaya Strain 139SI Using Different Carbon Sources and Surfactants

Mohaini

Farid

Muzammal

et al. 2022

Applied Microbiology

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Microbial lipase is one of the major sources of the enzyme that has been broadly exploited in the food, detergent, and pharmaceutical industries due to its high catalytic activity, high yield, and environmental friendliness and cost-effectiveness. Therefore, the aim of this study was to optimize the medium for the submerged fermentation for lipase production by a novel strain, Bacillus salmalaya strain 139SI. The media subjected to lipase production was Luria Bertani (LB) with different carbon sources and surfactants supplemented to determine which would give the highest lipase activity of Bacillus salmalaya. The Lipase activity of the supernatant containing lipase enzyme was ddetermined using the titrimetric method with hydrolysis reaction. Results showed that the olive oil that was used as a carbon source, induced the highest lipase activity (11.0 U/mL) compared to sunflower oil (9.6 U/mL) and cooking oil waste (7.8 U/mL). For surfactants, LB medium supplemented with tween 80 enhanced higher lipase activity (6.8 U/mL) compared to tween 20 (6.0 U/mL) and sodium dodecyl sulphate (SDS) (2.0 U/mL). Thus, it can be concluded that submerged fermentation allows optimization of the culture medium whereby, among carbon sources, olive oil induced the highest lipase production, whereas Tween 80 was the best lipase inducer compared to other surfactants.

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Section: Discussionmentioning

confidence: 99%

Enhancing Lipase Production of Bacillus salmalaya Strain 139SI Using Different Carbon Sources and Surfactants

Mohaini

Farid

Muzammal

et al. 2022

Applied Microbiology

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Optimization of novel halophilic lipase production by Fusarium solani strain NFCCL 4084 using palm oil mill effluent

Geoffry

Achur

2018

Journal of Genetic Engineering and Biotechnology

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Among different sources of lipases, fungal lipases have continued to attract a wide range of applications. Further, halophilic lipases are highly desirable for biodiesel production due to the need to mitigate environmental pollution caused as result of extensive use of fossil fuels. However, currently, the high production cost limits the industrial application of lipases. In order to address this issue, we have attempted to optimize lipase production by Fusarium solani NFCCL 4084 and using palm oil mill effluent (POME) based medium. The production was optimized using a combinatory approach of Plackett-Burman (PB) design, one factor at a time (OFAT) design and face centred central composite design (FCCCD). The variables (malt extract, (NH4)2SO4, CaCl2, MgSO4, olive oil, peptone, K2HPO4, NaNO3, Tween-80, POME and pH) were analyzed using PB design and the variables with positive contrast coefficient were found to be K2HPO4, NaNO3, Tween-80, POME and pH. The significant variables selected were further analyzed for possible optimum range by using OFAT approach and the findings revealed that K2HPO4, NaNO3, and Tween-80 as the most significant medium components, and thus were further optimized by using FCCCD. The optimum medium yielded a lipase with an activity of 7.8 U/ml, a significant 3.2-fold increase compared to un-optimized medium. The present findings revealed that POME is an alternative and suitable substrate for halophilic lipase production at low cost. Also, it is clearly evident that the combinatory approach employed here proved to be very effective in producing high activity halophilic lipases, in general.

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The use of response surface methodology for enhanced production of a thermostable bacterial lipase in a novel yeast system

Abu

Mohammad

Oslan

et al. 2020

Preparative Biochemistry & Biotechnology

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Optimization of Process Variables for Extracellular Lipase Production from Emericella nidulans NFCCI 3643 Isolated from Palm Oil Mill Effluent (POME) Dump Sites Using OFAT Method

Cited by 7 publications

References 28 publications

Enhancing Lipase Production of Bacillus salmalaya Strain 139SI Using Different Carbon Sources and Surfactants

Enhancing Lipase Production of Bacillus salmalaya Strain 139SI Using Different Carbon Sources and Surfactants

Optimization of novel halophilic lipase production by Fusarium solani strain NFCCL 4084 using palm oil mill effluent

The use of response surface methodology for enhanced production of a thermostable bacterial lipase in a novel yeast system

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