Enhancing Lipase Production of Bacillus salmalaya Strain 139SI Using Different Carbon Sources and Surfactants

Mohaini, Mohammed Al; Farid, Arshad; Muzammal, Muhammad; Ghazanfar, Shakira; Dadrasnia, Arezoo; Alsalman, Abdulkhaliq J.; Hawaj, Maitham A. Al; Alhashem, Yousef N.; Ismail, Salmah

doi:10.3390/applmicrobiol2010017

Cited by 13 publications

(4 citation statements)

References 16 publications

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“…The auto-aggregation property of bacterial isolates allows them to adhere to the epithelial lining of intestines, which is considered as a beneficial property because it prevents the flush out of bacterial strains from the body through peristalsis. The auto-aggregation percentages of isolated strains increased with time which is consistent with previous findings [49,50]. In a study conducted by Abid et al, it was revealed that Lactobacilli strains, i.e., NMCC-14, exhibit the highest auto-aggregation properties, i.e., 47.55 ± 0.08.…”

Section: Discussionsupporting

confidence: 89%

Probiotic and Antioxidant Potential of the Lactobacillus Spp. Isolated from Artisanal Fermented Pickles

Akmal

Ghori²,

Elasbali³

et al. 2022

Fermentation

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The present study was based on bacterial isolation with probiotic potential from artisanal fermented pickles. A total of 36 bacterial strains were isolated from 50 different artisanal fermented pickle samples. Nine isolates with promising probiotic potential (PCR99, PCR100, PCR118, PCR119, PCR121, PCR125, PCR137, PCR140 and PCR141) were selected. The strains showed varied protease, amylase, lipase and cellulase patterns. The isolated strains displayed varied responses towards various antibiotic classes, i.e., PCR140 showed resistance to penicillin G, polymyxin B, Metronidazole and Streptomycin. PCR140 showed highest resistance to bile salt concentrations (0.3% and 0.5%) and acidic conditions (pH 3 and pH 4) when exposed to mimicked gastrointestinal conditions. The cell viability against enzymes produced in stomach and intestines showed different patterns as pepsin was in the range of 94.32–91.22%, pancreatic resistance 97.32–93.11% and lysozyme resistance was detected at 99.12–92.55%. Furthermore, the auto-aggregation capability of isolated strains was in the range of 46.11–33.33% and cell surface hydrophobicity was in the range of 36.55–31.33%. PCR 140 showed maximum antioxidant activity in lyophilized cells as well as probiotic potential. A phylogenetic analysis based on 16S rRNA gene sequencing confirmed that PCR140 (NMCC91) with higher in vitro probiotic and antioxidant potential belongs to the genus Lactobacillus with 97% similarity with Lacticaseibacillus paracasei. This work demonstrated that the isolate PCR 140 (NMCC91) is suitable for use in food and medical industries.

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Section: Discussionsupporting

confidence: 89%

Probiotic and Antioxidant Potential of the Lactobacillus Spp. Isolated from Artisanal Fermented Pickles

Akmal

Ghori²,

Elasbali³

et al. 2022

Fermentation

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“…gessardii [59], and B. subtilis [47] maximally secreted lipolytic enzyme when the production media were constituted with tween 80, olive oil, palm oil, beef tallow, and waste cooking oil, respectively. Among the surfactants, tween 80, which showed the maximum inducible effect in the present study, has been reported to be a potent lipase inducer compared to tween 20, sodium dodecyl sulfate and triton X-100 [60].…”

Section: Discussionmentioning

confidence: 51%

Delipidation of Chicken Feathers by Lipolytic Bacillus Species Isolated from River-Borne Sediments

Shiri,

Nnolim,

Nwodo

2023

Clean Technol.

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Though the keratin content of chicken feathers is being explored for many potential uses, the crude lipid content of the biomass significantly hinders the valorization processes. Therefore, this study explored the potential of bacteria isolated from sediment for lipolytic properties. Sediment-associated strains were evaluated for lipolytic activity on tween 80–peptone agar. The best lipolytic bacterium was used to break down the lipid content of chicken feathers. The results showed that out of six bacterial strains with variable lipolytic activity, strain TTs1 showed the largest zone of precipitate around the colony, which is why it was selected and identified as Bacillus sp. TTs1. The maximum lipase production of 1530.5 U/mL by strain TTs1 was achieved at 96 h post-fermentation, with optimal process conditions of initial pH (10), incubation temperature (45 °C), agitation speed (140 rpm), inoculum size (2% v/v) and tween 80 (10% v/v). The total free fatty acid (0.58%) was liberated from chicken feathers at a concentration of 6% (w/v). Crude fat extraction from both untreated and TTs1-pretreated chicken feathers showed fat contents of 2.1 ± 0.42% and 0.92 ± 0.13%, respectively. The findings of this study highlight the biotechnological relevance of strain TTs1 in lipase production and the sustainable delipidation of lipid-rich bioresources.

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“…Fe-MIL-88NH 2 was prepared according to the previously reported protocol [39]. Briefly, FeCl 3 •6H 2 O (0.187 g; 0.692 mmol) and 2-aminoterephthalicacid (0.126 g; 0.692 mmol) were dissolved in 15 mL of DMF followed by the addition of 3.45 mmol of acetic acid into the resulting mixture.…”

Section: Methodsmentioning

confidence: 99%

Characterization and Antibacterial Evaluation of Biodegradable Mannose-Conjugated Fe-MIL-88NH2 Composites Containing Vancomycin against Methicillin-Resistant Staphylococcus aureus Strains

et al. 2022

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The emergence of bacterial resistance has increased the economic burden of infectious diseases dramatically during the previous few decades. Multidrug resistance (MDR) is difficult to cure in both Gram-negative and positive bacteria and is often incurable with traditional and broad-range antibiotics. Therefore, developing techniques to increase the antibacterial activity of therapeutic drugs is essential. Metal-organic frameworks (MOFs) are extremely versatile hybrid materials made of metal ions coupled via organic bridging ligands. They have been widely used as an excellent vehicle for drug delivery due to their low toxicity, biodegradability, and structural stability upon loading and functionalization. The present study focused on the synthesis of mannose (MNS)-coated MOFs with enhanced surface contact with S. aureus cells. The MNS coating on the surface of MOFs enhances their adherence to bacteria by binding to lectins present on the bacterial cell, resulting in improved VCM cellular penetration and activity against resistant bacteria. Various techniques, including atomic force microscopy, DLS, TGA, FT-IR, and DSC, were employed to analyze MNS-coated MOFs. They were also evaluated for their efficacy against resistant S. aureus. The results indicated that when VCM was loaded into MNS-coated MOFs, their bactericidal activity rose dramatically, resulting in the greater suppression of resistant S. aureus. AFM investigation of S. aureus strains demonstrated total morphological distortion after treatment with MNS-coated drug-loaded MOFs. The results of this work suggest that MNS-coated MOFs may be effective for reversing bacterial resistance to VCM and open new pathways for improving antibiotic therapy for diseases associated with MDR.

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Enhancing Lipase Production of Bacillus salmalaya Strain 139SI Using Different Carbon Sources and Surfactants

Cited by 13 publications

References 16 publications

Probiotic and Antioxidant Potential of the Lactobacillus Spp. Isolated from Artisanal Fermented Pickles

Probiotic and Antioxidant Potential of the Lactobacillus Spp. Isolated from Artisanal Fermented Pickles

Delipidation of Chicken Feathers by Lipolytic Bacillus Species Isolated from River-Borne Sediments

Characterization and Antibacterial Evaluation of Biodegradable Mannose-Conjugated Fe-MIL-88NH2 Composites Containing Vancomycin against Methicillin-Resistant Staphylococcus aureus Strains

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