Optimization of novel halophilic lipase production by Fusarium solani strain NFCCL 4084 using palm oil mill effluent

Abstract

Among different sources of lipases, fungal lipases have continued to attract a wide range of applications. Further, halophilic lipases are highly desirable for biodiesel production due to the need to mitigate environmental pollution caused as result of extensive use of fossil fuels. However, currently, the high production cost limits the industrial application of lipases. In order to address this issue, we have attempted to optimize lipase production by Fusarium solani NFCCL 4084 and using palm oil mill effluent (POME) based medium. The production was optimized using a combinatory approach of Plackett-Burman (PB) design, one factor at a time (OFAT) design and face centred central composite design (FCCCD). The variables (malt extract, (NH4)2SO4, CaCl2, MgSO4, olive oil, peptone, K2HPO4, NaNO3, Tween-80, POME and pH) were analyzed using PB design and the variables with positive contrast coefficient were found to be K2HPO4, NaNO3, Tween-80, POME and pH. The significant variables selected were further analyzed for possible optimum range by using OFAT approach and the findings revealed that K2HPO4, NaNO3, and Tween-80 as the most significant medium components, and thus were further optimized by using FCCCD. The optimum medium yielded a lipase with an activity of 7.8 U/ml, a significant 3.2-fold increase compared to un-optimized medium. The present findings revealed that POME is an alternative and suitable substrate for halophilic lipase production at low cost. Also, it is clearly evident that the combinatory approach employed here proved to be very effective in producing high activity halophilic lipases, in general.

Orchestration an extracellular lipase production from Aspergillus niger MYA 135: biomass morphology and fungal physiology

“…On the contrary, the presence of 0.05 g/L CaCl 2 exhibited a highly negative impact on the response (E = − 7.390). Similarly, Geoffry and Achur ( 2018a ), reported that the variable CaCl 2 influences negatively on the production of a lipase activity from Fusarium solani . Oppositely, the supplementation of this salt is favorable for the synthesis of a lipase activity using Rhizopus arrhizus MTCC 2233 (Rajendran and Thangavelu 2009 ).…”

“…On the contrary, the presence of 0.05 g/L CaCl 2 exhibited a highly negative impact on the response (E = − 7.390). Similarly, Geoffry and Achur (2018a), reported that the variable CaCl 2 influences negatively on the production of a lipase activity from Fusarium solani.…”

“…Thus, both fructofuranosidase and glucoamylase production by A. niger has been negatively correlated with an increasing the morphological number; while, a hypothetical correlation between the morphological number and productivity is proposed for citric acid and secondary metabolites (Cairns et al 2019). In the same way, the enzyme productions are influenced by physicochemical factors that should be optimized to reach a maximum yield (Geoffry and Achur 2018a). In other to control fungal morphology, several strategies have been reported.…”

Microbial lipases and their industrial applications: a comprehensive review

“…Due to their high transphosphatidylation and hydrolytic activities PLDs isolated from Actinomycete strains are commercially available and used in several industrialized procedures [ 110 ]. Mostly the bacterial genera for the production of lipases and phospholipases have been reconnoitered are Pseudomonas , Bacillus and Streptomyces , followed by Burkholderia , Chromobacterium , Achromobacter , Alcaligenes and Arthrobacter [ 111 ]. Some lipases producing microorganisms reveal new sources and applications of industrial enzymes as shown in Table 1 .…”

Enhanced halophilic lipase secretion by Marinobacter litoralis SW‐45 and its potential fatty acid esters release

“…Plackett‐Burmann (PB) experimental design is another statistical method that has been utilized for initial screening of the most important culture medium components and parameters among variables for the enhanced halophilic lipase secretion by Marinobacter litoralis SW‐45. Geoffry and Achur recently revealed that the combinatorial statistical optimization strategy enhanced the halophilic lipase secretion by 3.2‐fold, compared with the unoptimized experiment.…”