Optimization of Phospholipid Nanoparticle Formulations Using Response Surface Methodology

Abstract

The present study deals with the optimization of phospholipid liposome formulations to mimic red blood cells. Optimization of different concentrations of distearylphosphatidylcholine, dipalmitoylphosphatidylcholine, and phosphatidylserine at a fixed concentration of lecithin and Tween® 80 was done using response surface methodology. The optimized formulation produced liposomes with a particle size in the range of 112–196 nm. The optimized formulation shows low encapsulation efficiency at low levels of insulin but increases at higher loading levels. Formulated vesicles fulfill the size requirement for intravenous drug delivery. The present system is environmentally friendly with respect to biodegradability and biocompatibility.

Optimization of Conditions for Cyanidin-3-OGlucoside (C3G) Nanoliposome Production by Response Surface Methodology and Cellular Uptake Studies in Caco-2 Cells

“…Naeem used RSM to investigate the effects of phospholipid formulation on liposomes. In addition, Lu used RSM to study the effects of tea polyphenol/lecithin ratio and phosphate-buffered saline (PBS) pH on the preparation of tea polyphenol nanoliposomes [ 26 , 27 , 28 ].…”

Green Intelligent Nanomaterials by Design (Using Nanoparticulate/2D-Materials Building Blocks) Current Developments and Future Trends

“…Liposomal drugs are known to exhibit reduced toxicities while retaining enhanced efficacy compared to the free complements [39][40][41]. The phospholipid liposomes were reported mimicking red blood cells by optimizing concentrations of phosphatidylserine, di-stearylphosphatidylcholine, and dipalmitoylphosphatidylcholine for a fixed concentration of lecithin and Tween® 80 using response surface methodology resulting in 112-196 nm particle size with lower efficiency encapsulation at lower levels of insulin but increasing at higher levels fulfilling the requirement for intravenous drug delivery having biodegradable and biocompatible features [236].…”

Development of Crystal Violet encapsulation in pectin - Arabic gum gel microspheres

“…However, the mentioned systems are requiring chemical synthetic precursors (i.e., acrylamide, tetraethoxysilane) or materials produced by coal combustion (i.e., fly ash) which are not environmentally friendly. Also, CV was encapsulated in liposomes made of phospholipids and dicetyl phosphate and SDS micelles, in both methods organic solvents are used [13,14]. Those lipid structures are thermodynamically unstable.…”