J. Clin. Lab. Anal. volume 27, issue 6, P487-493 2013 DOI: 10.1002/jcla.21632 View full text
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Jasmina Obradovic, Vladimir Jurisic, Natasa Tosic, Jasminka Mrdjanovic, Branislav Perin, Sonja Pavlovic, Natasa Djordjevic

Abstract: In conclusion, EGFR promoter region is a difficult PCR target, but it could be amplified after optimization of MgCl2 concentration and annealing temperature in the presence of DMSO and the DNA template of acceptable concentration.