Optimization of nutritional conditions using a temporary immersion bioreactor system for the growth of Bletilla striata pseudobulbs and accumulation of polysaccharides

Zhang, Baoqian; Sarsaiya, Surendra; Pan, Xiaoguang; Jin, Leilei; Xu, Delin; Zhang, Benhou; Duns, Gregory J.; Shi, Jing‐Shan; Chen, Jishuang

doi:10.1016/j.scienta.2018.06.010

Cited by 18 publications

(10 citation statements)

References 32 publications

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“…Some authors have monitored the effect of different sucrose concentrations in the culture substrate on biomass production. In particular, Curcuma zedoaria, Zingiber zerumbet, Gynura procumbens, and Bletilla striata have showed that a higher concentration of sugar improves the performance of explants [52,62,73], whereas a significant increase in shoots number (13.05) of Bambusa vulgaris was noted in TIS with a sucrose-free medium [51].…”

Section: Medium Composition and Biomass Productionmentioning

confidence: 99%

Temporary Immersion System for Production of Biomass and Bioactive Compounds from Medicinal Plants

et al. 2021

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The cultivation of medicinal plants and the production of bioactive compounds derived from them are of fundamental importance and interest, not only at the pharmacological level but also in nutraceutical and cosmetic industries and in functional foods, as well as plant protection in agriculture. In order to respond adequately to the increased demands of the global market from a quantitative and qualitative point of view and to guarantee environmental sustainability of the productions, it is necessary to resort to innovation tools, such as tissue culture in vitro technology. Nowadays, it is well known that the cultivation through the Temporary Immersion System (TIS) in a bioreactor has considerable advantages both for the in vitro mass production of the plants and for the production of secondary metabolites. The present review focuses on the application of TIS during the last two decades to produce biomass and bioactive compounds from medicinal plants. Indeed, almost one hundred papers are discussed, and they particularly focus on the effects of the culture system, vessel design and equipment, immersion time and frequency, and substrate composition for 88 medicinal species in TIS bioreactor culture.

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Section: Medium Composition and Biomass Productionmentioning

confidence: 99%

Temporary Immersion System for Production of Biomass and Bioactive Compounds from Medicinal Plants

et al. 2021

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“…They are also some of the most vulnerable flowering plants, as numerous genera are endangered and nearly all genera are at risk of habitat harm and over-assortment [1]. China is a rich source of orchid plants, with over 1447 species, mostly located in the subtropical and tropical provinces in the southwest and south [1,2]. Many Dendrobium orchids are horticultural plants and have been used for profitable trade due to their flowering profusion, extensive variety of flower colors, shapes, sizes, and year-round producibility, along with long lifespan [3].…”

Section: Introductionmentioning

confidence: 99%

Molecular Identification of Endophytic Fungi and Their Pathogenicity Evaluation Against Dendrobium nobile and Dendrobium officinale

Sarsaiya

Jain

Fan

et al. 2020

IJMS

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Dendrobium are tropical orchid plants that host diverse endophytic fungi. The role of these fungi is not currently well understood in Dendrobium plants. We morphologically and molecularly identified these fungal endophytes, and created an efficient system for evaluating the pathogenicity and symptoms of endophytic fungi on Dendrobium nobile and Dendrobium officinale though in vitro co-culturing. ReThe colony morphological traits of Dendrobium myco-endophytes (DMEs) were recorded for their identification. Molecular identification revealed the presence of Colletotrichum tropicicola, Fusarium keratoplasticum, Fusarium oxysporum, Fusarium solani, and Trichoderma longibrachiatum. The pathogenicity results revealed that T. longibrachiatum produced the least pathogenic effects against D. nobile protocorms. In seedlings, T. longibrachiatum showed the least pathogenic effects against D. officinale seedlings after seven days. C. tropicicola produced highly pathogenic effects against both Dendrobium seedlings. The results of histological examination of infected tissues revealed that F. keratoplasticum and T. longibrachiatum fulfill Koch’s postulates for the existence of endophytes inside the living tissues. The DMEs are cross-transmitted inside the host plant cells, playing an important role in plant host development, resistance, and alkaloids stimulation.

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“…Various TIS designs have been reported; however, temporary immersion bioreactors (TIB ® ) or twin-jar bioreactors show advantages relative to other systems due to the broad availability of components, different capacity, and easy assembly (Aragón et al 2014;Georgiev et al 2014). Most micropropagation studies of orchids (Orchidaceae) using temporary immersion have involved the use of TIB ® (Murthy et al 2018;Shen and Hsu 2018;Zhang et al 2018). However, according to Murthy et al (2018), the nutritional requirements and particular parameters of TIS micropropagation should be tailored to each species studied.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Guarianthe skinneri (Bateman) Dressler et W. E. Higging in Temporary Immersion Systems

et al. 2020

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This study's aim was to establish a protocol for the micropropagation of G. skinneri using temporary immersion system (TIS). Different concentrations of 6-Benzylaminopurine (0, 1, 2, and 3 mg L −1 ), three different systems of cultivate semi-solid (SS) and liquid media under partial (PI) and temporary immersion systems (TIS), different compositions of the inorganic salts, and the number of subcultures were evaluated. The results showed a maximum of 16.56 shoots per explant obtained through TIS, adjusting all the parameters evaluated in our study. One higher number of shoots per explant was observed in the micropropagation of G. skinneri TIS compared to SS and PI. While the use of 3 mg L −1 of BAP + MS (Murashige and Skoog) media was better than 3 mg L −1 of BAP VW (Vacint and Went) for the generation of a greater number of shoots per explant, 6.33 and 2.72, respectively. The immersion frequency of 2 min every 4 h allowed the production to be scaled to 8.54 shoots per explant. While it was necessary to perform three subcultures every 30 days, to obtain 16.56 shoots per explant, a rooting phase was not required due to the generation of adventitious roots during the different subcultures. However, a phase of elongation of the regenerated plants with ½ MS + GA3 (gibberellic acid) was needed to guarantee 100% survival in the process of acclimatization. In conclusion, this plant production system can be applied for the commercial micropropagation of this species for ornamental purposes, as well as for its reintroduction in protected natural areas.

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Optimization of nutritional conditions using a temporary immersion bioreactor system for the growth of Bletilla striata pseudobulbs and accumulation of polysaccharides

Cited by 18 publications

References 32 publications

Temporary Immersion System for Production of Biomass and Bioactive Compounds from Medicinal Plants

Temporary Immersion System for Production of Biomass and Bioactive Compounds from Medicinal Plants

Molecular Identification of Endophytic Fungi and Their Pathogenicity Evaluation Against Dendrobium nobile and Dendrobium officinale

Micropropagation of Guarianthe skinneri (Bateman) Dressler et W. E. Higging in Temporary Immersion Systems

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