Optimization of monoclonal production of the glass anemone Aiptasia pallida (Agassiz in Verrill, 1864)

Leal, Miguel C.; Nunes, Cristina; Engrola, Sofía; Dinis, María Teresa; Calado, Ricardo

doi:10.1016/j.aquaculture.2012.03.035

Cited by 35 publications

(29 citation statements)

References 25 publications

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“…Another monoclonal line of A. pallida, originally collected in Bermuda, was used as well and hosted one of the unique strains of S. minutum (strain 3). All associations of A. pallida symbiosis were stable for over 1 year and kept in flow-through systems using FSW under normal growth conditions (26°C, ∼33‰, 150 μmol photons m −2 s −1 photosynthetic photon flux, 12 h light-dark cycle) and fed brine shrimp weekly (Leal et al, 2012). Associations with each symbiont's genotype were confirmed before experiments (see below).…”

Section: Animal Modelsmentioning

confidence: 97%

Symbiont type influences trophic plasticity of a model cnidarian–dinoflagellate symbiosis

Leal

Hoadley

Pettay

et al. 2015

Journal of Experimental Biology

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The association between cnidarians and photosynthetic dinoflagellates within the genus Symbiodinium is a prevalent relationship in tropical and subtropical marine environments. Although the diversity of Symbiodinium provides a possible axis for niche diversification, increased functional range and resilience to physical stressors such as elevated temperature, how such diversity relates to the physiological balance between autotrophy and heterotrophy of the host animal remains unknown. Here, we experimentally show interspecific and intraspecific variability of photosynthetic carbon fixation and subsequent translocation by Symbiodinium to the model cnidarian host Aiptasia pallida. By using a clonal anemone line harboring different species of Symbiodinium, we determined that symbiont identity influences trophic plasticity through its density, capacity to fix carbon, quantity of translocated carbon and ultimately the host's capacity to ingest and digest prey. Symbiont carbon translocation and host prey ingestion were positively correlated across symbiont combinations that consisted of different isoclonal lines of Symbiodinium minutum, while a combination with type D4-5 Symbiodinium displayed lower carbon translocation, and prey capture and digestion more similar to Aiptasia lacking symbionts. The absence of a shift toward greater heterotrophy when carbon translocation is low suggests that the metabolic demand of feeding and digestion may overwhelm nutritional stores when photosynthesis is reduced, and amends the possible role of animal feeding in resistance to or recovery from the effects of climate change in more obligate symbioses such as reef-building corals.

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Section: Animal Modelsmentioning

confidence: 97%

Symbiont type influences trophic plasticity of a model cnidarian–dinoflagellate symbiosis

Leal

Hoadley

Pettay

et al. 2015

Journal of Experimental Biology

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“…Anemones can be spawned in captivity with the manipulation of abiotic factors playing a key role to trigger the release of gametes (Scott & Harrison ), or propagated by fission or by fragmentation (Leal et al . ; Scott et al . ).…”

Section: Ornamentalsmentioning

confidence: 99%

“…are often slow growing and long lived (Fautin & Allen 1997), not being particularly prone to undergo asexual reproduction (Scott & Harrison 2009). Anemones can be spawned in captivity with the manipulation of abiotic factors playing a key role to trigger the release of gametes (Scott & Harrison 2008), or propagated by fission or by fragmentation (Leal et al 2012c;Scott et al 2014). Overall, the asexual reproduction of sea anemones is a viable lowtech approach to ensure their year round production.…”

Section: Othermentioning

confidence: 99%

Aquaculture of marine non‐food organisms: what, why and how?

Leal

Rocha

Rosa

et al. 2016

Reviews in Aquaculture

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Aquaculture is among the fastest growing industries in the world and the fastest in the food production sector. However, not all aquaculture organisms are produced for human consumption. Indeed, an important fraction of aquaculture products target a wide variety of applications, from pharmaceutical, cosmeceutical, ornamental and nutraceutical applications, as well as biofuel production, conservation efforts and academic purposes, among others. Despite the increasing importance of aquaculture for non‐food purposes, a comprehensive review covering the state of the art of this topic, its latest developments and future trends is missing. Here, we review what marine organisms are produced for non‐food purposes, as well as why and how they are produced. Instead of providing a thorough methodological review, production protocols are briefly summarized and an updated guide to the most relevant literature is provided. We overview current developments driving the aquaculture of non‐food organisms, underpin their most significant applications and highlight future prospects for non‐food aquaculture.

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“…The majority of the papers presented short timeframes or even a single observation, although some presented 8 or 17 years of monitoring (Leão et al, 2008;Kelmo and Attrill, 2013;Kelmo et al, 2014). About 90% of the events were recorded in the Northeastern region (Banha et al, in prep), probably due to its high diversity and higher number of coral reefs located within Marine Protected Areas.…”

Section: Introductionmentioning

confidence: 99%

“…The use of the upside-down benthic jellyfish Cassiopea andromeda may also be an alternative and relevant model host organism. They present many advantages since they can be easily maintained and bred in laboratory (Hofmann et al, 1996;Leal et al, 2012). The association between C. andromeda and Symbiodinium seems to be obligatory (Rowan et al, 1997;Mellas et al, 2014;Freeman et al, 2016) and symbiont acquisition is similar to that of the majority of cnidarians (Kinzie and Chee, 1979;Lampert, 2016).…”

Section: Introductionmentioning

confidence: 99%

Experimental effects of multiple thermal stress events on chlorophyll-a content and size of <i>Cassiopea andromeda</i> and the role of heterotrophic feeding and <i>Symbiodinium</i> concentration

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Optimization of monoclonal production of the glass anemone Aiptasia pallida (Agassiz in Verrill, 1864)

Cited by 35 publications

References 25 publications

Symbiont type influences trophic plasticity of a model cnidarian–dinoflagellate symbiosis

Symbiont type influences trophic plasticity of a model cnidarian–dinoflagellate symbiosis

Aquaculture of marine non‐food organisms: what, why and how?

Experimental effects of multiple thermal stress events on chlorophyll-a content and size of <i>Cassiopea andromeda</i> and the role of heterotrophic feeding and <i>Symbiodinium</i> concentration

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