Optimization of low ring polycylic aromatic biodegradation

Abstract

Abstract. Polycyclic aromatic hydrocarbons (PAHs) are recalcitrance and persistence that finally turn into problematic environmental contaminants. Microbial degradation is considered to be the primary mechanism of PAHs removal from the environment due to its organic criteria. This study is carried out to optimize degradation process of low ring PAHs. Bacteria used in this study was isolated from sludge collected from Kolej Mawar, Universiti Teknologi MARA, Shah Alam, Selangor. Working condition namely, substrate concentration, bacteria concentration, pH and temperature were optimized. PAHs in the liquid sample was extracted by using solid phase microextractio equipped with a 7µm polydimethylsiloxane (PDMS) SPME fibr . Removal of PAHs were assessed by measuring PAHs concentration using GC-FID. Results from the optimization study of biodegradation indicated that maximum rate of PAHs removal occurred at 100 mgL -1 of PAHs, 10% bacteria concentration, pH 7.0 and 30 o C. These working condition had proved the effectiveness of using bacteria in biodegradation process of PAHs.

Microbial communities in pesticide-contaminated soils in Kyrgyzstan and bioremediation possibilities

“…Working conditions, namely, substrate concentration, bacteria concentration, pH, and temperature, have to be optimized. Results from the optimization study of biodegradation indicated that the maximum rate of PAH removal occurred at 100 mg L −1 of PAHs, 10% bacteria concentration, pH 7.0, and 30 °C (Othman et al 2016 ). For the degradation of pesticides, bacteria must possess enzymes that cleave the ring of the aromatic halogen compound (PCP) (Arora et al 2010 ; Arora and Bae 2014 ; Zhou et al 2008 ).…”

Studi Metagenomik Sampel Perairan yang Diperkaya dari Wilayah Hilir Sungai Citarum dan Potensinya sebagai Agen Bioremediasi

“…Umumnya, bakteri perairan tumbuh baik pada kondisi perairan dengan pH 6,5-7,5 (Respati, 2017) dan suhu 20 hingga 40°C (Supriyati & Rahayu, 2011). Nilai pH menjadi faktor yang memengaruhi proses degradasi logam berat dan poliaromatik hidrokarbon oleh mikroba dan umumnya mampu mendegradasi dengan baik pada kisaran 7,0-7,5 (Othman et al, 2016). Kadar oksigen terlarut (DO) digunakan mikroorganisme perairan untuk metabolisme guna menghasilkan energi (Jurtshuk, 1996) dan merombak polutan organik (Bara'padang et al, 2019).…”