Optimization of low-cost solid-state fermentation media for the production of thermostable lipases using agro-industrial residues as substrate in culture of Bacillus amyloliquefaciens

Mazhar, Haniya; Ullah, Ikram; Ali, Umair; Abbas, Naaz; Hussain, Zahid; Ali, Syed Shahid; Zhu, Hongxin

doi:10.1016/j.bcab.2022.102559

Cited by 17 publications

(2 citation statements)

References 64 publications

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“…SHI-160 was achieved when grown in the presence of yeast extract as the sole nitrogen source. Similar reports of better lipase production using yeast extract alone were reported for Bacillus amyloliquefaciens (Mazhar et al, 2023 ), Burkholderia multivorans (Gupta et al, 2007 ), Trichosporon asahii MSR 54 (Kumar and Gupta, 2008 ), and Pseudomonas aeruginosa (Ilesanmi et al, 2020 ). Since yeast extract is rich in vitamins, peptides, amino acids, phosphate, and other essential minerals (Li et al, 2011 ; Tomé, 2021 ; Tao et al, 2023 ), it provides the nutritional requirements of the organism for optimum growth and enzyme production.…”

Section: Discussionsupporting

confidence: 72%

A thermostable organic solvent-tolerant lipase from Brevibacillus sp.: production and integrated downstream processing using an alcohol-salt-based aqueous two-phase system

Leykun,

Johansson,

Vetukuri

et al. 2023

Front. Microbiol.

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Lipases are used for the synthesis of different compounds in the chemical, pharmaceutical, and food industries. Most of the reactions are carried out in non-aqueous media and often at elevated temperature, requiring the use of organic solvent-tolerant thermostable lipases. However, most known lipases are not stable in the presence of organic solvents and at elevated temperature. In this study, an organic solvent-tolerant thermostable lipase was obtained from Brevibacillus sp. SHI-160, a moderate thermophile isolated from a hot spring in the East African Rift Valley. The enzyme was optimally active at 65°C and retained over 90% of its activity after 1 h of incubation at 70°C. High lipase activity was measured in the pH range of 6.5 to 9.0 with an optimum pH of 8.5. The enzyme was stable in the presence of both polar and non-polar organic solvents. The stability of the enzyme in the presence of polar organic solvents allowed the development of an efficient downstream processing using an alcohol-salt-based aqueous two-phase system (ATPS). Thus, in the presence of 2% salt, over 98% of the enzyme partitioned to the alcohol phase. The ATPS-recovered enzyme was directly immobilized on a solid support through adsorption and successfully used to catalyze a transesterification reaction between paranitrophenyl palmitate and short-chain alcohols in non-aqueous media. This shows the potential of lipase SHI-160 to catalyze reactions in non-aqueous media for the synthesis of valuable compounds. The integrated approach developed for enzyme production and cheap and efficient downstream processing using ATPS could allow a significant reduction in enzyme production costs. The results also show the potential of extreme environments in the East African Rift Valley as sources of valuable microbial genetic resources for the isolation of novel lipases and other industrially important enzymes.

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Section: Discussionsupporting

confidence: 72%

A thermostable organic solvent-tolerant lipase from Brevibacillus sp.: production and integrated downstream processing using an alcohol-salt-based aqueous two-phase system

Leykun,

Johansson,

Vetukuri

et al. 2023

Front. Microbiol.

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“…Inoculum size for lipase production in the range of 1-5% was reported by several studies where the highest lipase production was observed till inoculum size of 5% and inoculum size of 8% reduced the lipase production. [24,41,42]. OFAT experiments were performed to explore the effect of individual variables on product formation.…”

Section: One Factor At a Time Analysismentioning

confidence: 99%

Response surface optimization of solvent tolerant cold active lipase production by Pseudomonas sp. VITCLP4

Lakshimi,

Kavitha

2023

Preprint

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The yield of solvent-tolerant cold active lipase from halo-tolerant Pseudomonas sp. VITCLP4 was improved by applying statistical methods to precisely combine medium components and other conditions. Two-level Plackett–Burman (PB) design was utilized to screen the most significant variables from a set of eleven variables that influence lipase production. Analysis of variance revealed that Tween-80, peptone, and KH2PO4 were statistically significant. Optimum levels of selected variables were ascertained by one-factor-at-a-time (OFAT) analysis. The optimum values and interactive effects of the selected three components were established by response surface methodology (RSM) based on the face-centered central composite design (FCCCD) using a three-level-three-factor approach. The optimized medium containing Tween-80, 0.5% (v/v), peptone, 0.75% (w/v), KH2PO4, 0.05% (w/v), yeast extract, 0.1% (w/v), olive oil, 0.2% (v/v) and inoculum size, 0.2% (v/v) resulted in maximum lipase production of 1342 Units ml− 1 min− 1 with 7.8 fold increase in the yield. This study provides insights into enhancing the lipase production statistically with optimized resources that can be utilized in bioprocess studies and industrial applications.

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Production of Gibberellic Acid by Solid-State Fermentation Using Wastes from Rice Processing and Brewing Industry

Pinheiro

Wancura

Brondani

et al. 2023

Appl Biochem Biotechnol

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Optimization of low-cost solid-state fermentation media for the production of thermostable lipases using agro-industrial residues as substrate in culture of Bacillus amyloliquefaciens

Cited by 17 publications

References 64 publications

A thermostable organic solvent-tolerant lipase from Brevibacillus sp.: production and integrated downstream processing using an alcohol-salt-based aqueous two-phase system

A thermostable organic solvent-tolerant lipase from Brevibacillus sp.: production and integrated downstream processing using an alcohol-salt-based aqueous two-phase system

Response surface optimization of solvent tolerant cold active lipase production by Pseudomonas sp. VITCLP4

Production of Gibberellic Acid by Solid-State Fermentation Using Wastes from Rice Processing and Brewing Industry

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