Optimization of laccase production in the white-rot fungusPleurotus ostreatus(ACCC 52857) induced through yeast extract and copper

Abstract

Different inducers for laccase production in Pleurotus ostreatus (ACCC 52857) were screened: carbon and nitrogen source, phenolic compounds and metal ions. Among the tested substances, yeast extract and copper showed the strongest effect on laccase activity. Laccase activity increased during the early phase of cultivation in the presence of yeast extract, peaking on the 6th day and decreasing thereafter. Copper-induced laccase activity increased both in a dose-dependent and a time-dependent manner. The highest laccase activity was obtained with 2 mmol/L Cu 2C , while the mycelial growth was inhibited approximately 27%. Thus, the time-dependent effect of copper on laccase activity was examined. The results showed that the best laccase production was induced when copper was added during the mid-logarithmic phase of cultivation (the 5th day). A positive synergistic effect of yeast extract and copper on the laccase production was observed. Laccase activity dramatically increased upon the addition of copper to medium containing 1% yeast extract on the 5th day of cultivation. The highest activity (8533.33 § 1228.94 U/mL) was observed on the 13th day of cultivation, increased more than 80 folds compared to the original level.

Optimization of Laccase Production by Locally Isolated Trichoderma muroiana IS1037 Using Rubber Wood Dust as Substrate

“…The highest laccase activity of 65.0 U/mL was achieved from 2 mM copper sulfate during the fourth day of fermentation, while the lowest laccase activity was captured around 5.8 U/mL in the control test (without any CuSO4). This result was quite similar to another report for laccase production by Pleurotus ostreatus (ACCC 52857), which found increased laccase activity in 2 mM copper sulfate (Changwei et al 2016). Therefore, the addition of copper sulfate (2 mM) to the culture medium had a noticeable impact on laccase production.…”

COPPER IMPROVES THE PRODUCTION OF LACCASE BY Pleurotus sajor-caju WITH ABILITY TO GROW ON EFFLUENTS OF THE CITRUS INDUSTRY

“…The inducing effect of copper on laccase activity of P. sajor-caju was both dose-and time-dependent, as it was also observed by Zhu et al (2016) regarding P. ostreatus. Our results indicate that smaller amounts of Cu 2+ gave better results in shorter times.…”

Laccase activity of the ascomycete fungus Nectriella pironii and innovative strategies for its production on leaf litter of an urban park

“…Efforts have been made to produce large amounts of laccases at lower costs with the use of recombinant organisms or screening for naturally available hypersecretory strains. To enhance laccase production, different strategies can also be applied, such as optimization of culture conditions or use of lignocellulose materials as substrates [10,13,40]. Increased secretion of laccase by fungi when cultured in media supplemented with copper or different aromatic compounds has been well documented [41,42].…”

“…Increase of enzyme biosynthesis through modification of the culture medium composition, changing the processing conditions, and supplementation with chemical inducers, such as metal ions and aromatic compounds, is a well-known solution for this problem. However, few studies indicate synergistic stimulation of laccase production by metal ions and aromatic compounds [9,10]. Also, utilizing bio-waste to obtain an enzyme can reduce production cost while generating higher concentrations of products [11].…”