Optimization of Laccase Production by Locally Isolated Trichoderma muroiana IS1037 Using Rubber Wood Dust as Substrate

Jaber, Saja Mahdey; Shah, Umi Kalsom Md; Asa’ari, Ainun Zuriyati Mohamed; Ariff, Arbakariya B.

doi:10.15376/biores.12.2.3834-3849

Cited by 17 publications

(12 citation statements)

References 15 publications

(15 reference statements)

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“…Fungal laccases are reported to be generally active at low pH values (pH 3.0-5.0) (30) . However, results of present study disagree with results reported earlier with maximum enzyme production at pH 4.5 (30,31) .…”

Section: Optimization Of Ph and Temperaturecontrasting

confidence: 99%

“…Greater than 10mM copper repress enzyme production. Present findings https://www.indjst.org/ did not support recent report on Trichoderma muroiana IS1037 (31) . High concentration of copper acts as an inhibitor of fungal growth which is a key component in many fungicides (11) .…”

Section: Effect Of Inducers and Copper Ion Concentrationcontrasting

confidence: 99%

“…Laccase production was increased 18 fold compared to that of unoptimized medium (5.5 U/mL) with the maximum production of 98.6 U/mL on 96 h of incubation [ Figure 12]. Maximum laccase production has been reported on 96 hrs of incubation (17,18,31) . Use of single parameter at a time approach for optimization of cultural conditions has been reported earlier for efficient laccase production (35,36) .…”

Section: Time Course Study For Maximum Laccase Productionmentioning

confidence: 99%

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Orange peel as an inducer for Laccase production in a novel fungal strain peyronellaea pinodella BL-3/4 and optimization of its cultural parameters by single parameter approach

lowast¹,

Bhaskaran²

2020

IJST

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Section: Optimization Of Ph and Temperaturecontrasting

confidence: 99%

Section: Effect Of Inducers and Copper Ion Concentrationcontrasting

confidence: 99%

Section: Time Course Study For Maximum Laccase Productionmentioning

confidence: 99%

See 1 more Smart Citation

Orange peel as an inducer for Laccase production in a novel fungal strain peyronellaea pinodella BL-3/4 and optimization of its cultural parameters by single parameter approach

lowast¹,

Bhaskaran²

2020

IJST

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“…In accordance with the previous report ( Eisen et al, 1998 ) the cluster analysis revealed clear patterns of microbial properties. Similar research on FF plates ® showing metabolic differences of closely related fungi were recently performed by, e.g., ( Janusz et al, 2015 ; Jaber et al, 2017 ; Pinzari et al, 2017 ). Regardless of the restriction criterion in cluster analysis, P. setifera isolates revealed dichotomy clustering in A and B groups ( Figure 4A ).…”

Section: Resultssupporting

confidence: 78%

Metabolic and Genetic Properties of Petriella setifera Precultured on Waste

et al. 2018

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Although fungi that belong to Petriella genus are considered to be favorable agents in the process of microbial decomposition or as plant endophytes, they may simultaneously become plant pests. Hence, nutrition factors are supposed to play an important role. Therefore, it was hypothesized that Petriella setifera compost isolates, precultured on three different waste-based media containing oak sawdust, beet pulp (BP) and wheat bran (WB) will subsequently reveal different metabolic properties and shifts in genetic fingerprinting. In fact, the aim was to measure the influence of selected waste on the properties of P. setifera. The metabolic potential was evaluated by the ability of five P. setifera strains to decompose oak sawdust, BP and WB following the MT2 plate® method and the catabolic abilities of the fungus to utilize the carbon compounds located on filamentous fungi (FF) plates®. Genetic diversity was evaluated using Amplified Fragment Length Polymorphism analysis performed both on DNA sequences and on transcript-derived fragments. P. setifera isolates were found to be more suitable for decomposing waste materials rich in protein, N, P, K and easily accessible sugars (as found in WB and BP), than those rich in lignocellulose (oak sawdust). Surprisingly, among the different waste media, lignocellulose-rich sawdust-based culture chiefly triggered changes in the metabolic and genetic features of P. setifera. Most particularly, it contributed to improvements in the ability of the fungus to utilize waste-substrates in MT2 plate® and two times increase the ability to catabolize carbon compounds located in FF plates®. Expressive metabolic properties resulting from being grown in sawdust-based substrate were in accordance with differing genotype profiles but not transcriptome. Intraspecific differences among P. setifera isolates are described.

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“…Apart from this various inducer were also added at a concentration of (0.02% w/v) including copper sulfate, tannic acid, catechol, hydroquinone, acetaminophen, pyrogallol that affected the productivity of enzyme. Higher laccase activity was obtained at the optimum pH of 6.0and incubation temperature of 30 °C and 35 °C 19 .…”

Section: Fig 1: Oxidation Of (A) Phenolic Subunits Of Lignin By Laccmentioning

confidence: 94%

Untitled

2020

IJPSR

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Laccase belongs to polyphenol oxidases, which is a very wide family of copper-containing atoms in the center, which serves as the catalytic site. It is biochemically a glycoprotein with molecular mass being between 50kda to 130kda and 30% carbohydrate content, in addition to this it has three types of copper atoms. It is one of the most important enzymes due to its role in the food industry. It acts as a biosensor where it can detect morphine, codeine, catecholamine, and many other enzymes. In insects, it performs the function of cuticle sclerotization. Moreover, laccase is safe for human consumption. The most important factor which has prevented laccase from being insanely famous is the cost that is associated with using a very large quantity of laccase. Laccases can be extracted from almost all kinds of white-rot fungi as well as from various other fungi species such as Pycnoporus cinnabarinus, Gloeophyllum trabeum but not in any brown-rot species except Coniophora puteana as was recently discovered. Aspergillus niger, Aspergillus oryzae and Trichoderma reesei also produce laccases due to the laccase producing genes introduced in them. Botryo sphaeria is a true laccase producing fungi, not only this, it can also be extracted from plants, bacteria such as Escherichia coli, Bacillus halodurans but not much work has been done on any other organism except fungi. Bacterial laccase is highly thermos-tolerant and functions in neutral to alkaline conditions, while fungal laccase, on the other hand, does not function at high temperature and pH. INTRODUCTION: Enzymes play a major role in the metabolism of all living organisms. Extracellular enzymes have gained great importance in the industrial sector. Among those, laccase is one of the most used enzymes due to its applications in various fields.

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Optimization of Laccase Production by Locally Isolated Trichoderma muroiana IS1037 Using Rubber Wood Dust as Substrate

Cited by 17 publications

References 15 publications

Orange peel as an inducer for Laccase production in a novel fungal strain peyronellaea pinodella BL-3/4 and optimization of its cultural parameters by single parameter approach

Orange peel as an inducer for Laccase production in a novel fungal strain peyronellaea pinodella BL-3/4 and optimization of its cultural parameters by single parameter approach

Metabolic and Genetic Properties of Petriella setifera Precultured on Waste

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