COPPER IMPROVES THE PRODUCTION OF LACCASE BY Pleurotus sajor-caju WITH ABILITY TO GROW ON EFFLUENTS OF THE CITRUS INDUSTRY

Fonseca, María Isabel; Molina, M.; Benítez, Silvana Florencia; Tejerina, Marcos Raúl; Velázquez, Juan Ernesto; Sadañoski, Marcela Alejandra; Zapata, Pedro Darío

doi:10.20937/rica.2020.36.53368

Cited by 6 publications

(3 citation statements)

References 41 publications

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“…The fits obtained to Eqn 1 were similar to that obtained evaluating the inhibitory effect of polychlorinated biphenyls on basidiomycetes fungi (Sadañoski et al 2018) and the study of the fungal inhibition effect of olive mill wastewater (Bevilacqua et al 2017). Also, the same model was used to test the tolerance of the citrus industry effluents on a white-rot fungus (Fonseca et al 2020).…”

Section: Discussionsupporting

confidence: 60%

Bioproduction of α‐terpineol and R‐(+)‐limonene derivatives by terpene‐tolerant ascomycete fungus as a potential contribution to the citrus value chain

Velázquez

Sadañoski

Zapata

et al. 2020

J. Appl. Microbiol.

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Aims: The aims of this article were to select fungal species with high tolerance and high growth rate in mediums supplemented with limonene and citrus essential oils (CEOs), and to test the bioconversion capability of the chosen isolates for the bioproduction of aroma compounds. Methods and Results: Based on the use of predictive mycology, 21 of 29 isolates were selected after assaying R-(+)-limonene and CEO tolerance (10 g l −1). With a dendrogram divisive coefficient of 0Á937, the subcluster two with isolates Aspergillus niger LBM 055, Penicillium sp. LBM 150, Penicillium sp. LBM 151 and Penicillium sp. LBM 154 gathered the highest tolerance and mycelia growth speed. Ultrastructural analysis indicated that culture media containing limonene had no visible toxic activity that could promote morphological changes in the fungal cell wall. The biomass of A. niger LBM055 was distinctive in liquid media supplemented with R-(+)-limonene (0Á57 AE 0Á07 g) and it was selected to prove bioconversion capacity, under static and agitated conditions, and converted up to 98% of limonene, yielding a wide variety of products that were quantified by GC-FID. It was obtained at molecular weights less than limonene (64-100%), between limonene and αterpineol (12-72%) and greater than α-terpineol (2-48%). Conclusions: Aspergillus niger LBM 055, Penicillium sp. LBM 150, Penicillium sp. LBM 151 and Penicillium sp. LBM 154 showed to the highest tolerance and growth rate in mediums supplemented with R-(+)-limonene and orange and lemon essential oils. Particularly, A. niger LBM055, showed limonene bioconversion capability and produced different molecular weights compounds such us α-terpineol. Significance and Impact of the Study: Different bioproducts can be obtained by changing operative condition with the same fungus, and this bioprocess aspect is a significant approach to be adopted on industrial scale leading to the creation of new natural flavours and fragrance compositions.

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Section: Discussionsupporting

confidence: 60%

Bioproduction of α‐terpineol and R‐(+)‐limonene derivatives by terpene‐tolerant ascomycete fungus as a potential contribution to the citrus value chain

Velázquez

Sadañoski

Zapata

et al. 2020

J. Appl. Microbiol.

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“…An increase of 10-fold and 1.4-fold in laccase activity has been registered at 1 mM CuSO 4 in Pleurotus ostreatus [ 42 ] and white rot fungus WR-1 [ 43 ], respectively. Variations in terms of the effect of copper addition on laccase activity and isoenzymatic patterns from Pleurotus sajor-caju , as laccase activity increased 2.3 and 1.3-fold in cultures supplemented with 0.5 and 1 mM CuSO 4 , respectively, and correlation with two isoenzymes of 65 kDa and 35 kDa shown by zymogram analysis has been reported [ 44 ]. In contrast, greater laccase production has been observed with low copper concentrations, e.g., the production of laccase activity by Agaricus blazei U2-4 in the culture containing 150 µM CuSO 4 was 0.96-fold higher than that in the control medium, lacking copper [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Improvement of Laccase Production by Thielavia terrestris Co3Bag1. Enhancing the Bio-Catalytic Performance of the Native Thermophilic TtLacA via Immobilization in Copper Alginate Gel Beads

Gutiérrez-Antón

Santiago-Hernández

Rodríguez-Mendoza

et al. 2023

JoF

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A 32-fold increase in laccase activity production by the thermophilic biomass-degrading fungus T. terrestris Co3Bag1 was achieved when the microorganism was grown on a modified medium containing fructose, sodium nitrate, and copper. A 70 kDa laccase (TtLacA), produced under the above conditions, was purified, immobilized in copper alginate gel beads, and characterized. TtLacA, both free and immobilized enzymes, exhibited optimal activity at pH 3.0, at a temperature of 65 and 70 °C, respectively, although both displayed 70% of activity from 40 to 70 °C. Free and immobilized enzymes retained at least 80% of relative activity in the pH range from 3 to 4.6. Immobilized TtLacA manifested a 2.3-fold higher thermal stability than the free form of the enzyme at 60 and 70 °C. Immobilized TtLacA retained 95% initial activity for six consecutive reuse cycles at 60 °C, and also retained 86% of initial activity after 12 days of storage at 4 °C. Based on the biochemical features, thermophilic TtLacA may be an efficient enzyme for dye decolorization and other industrial applications at high temperatures or acidic conditions. This work represents the first report about the immobilization and biochemical characterization of a thermophilic laccase from a member of the genus Thielavia.

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“…The highest laccase enzyme elicitation occurred in presence of copper in white-rot fungi [47]. Copper supplementation (up to 0.5 mM) increased the laccase expression in Pleurotus sajor-caju when grown on industrial effluents [48]. Under solid-state fermentation (SSB) in Trametes versicolor, Cu supplementation in the basal medium induced laccase production [49].…”

Section: Discussionmentioning

confidence: 99%

Biosorption and Bioleaching of Heavy Metals from Electronic Waste Varied with Microbial Genera

et al. 2022

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Industrialization and technological advancements have led to the exploitation of natural resources and the production of hazardous wastes, including electronic waste (E-waste). The traditional physical and chemical techniques used to combat E-waste accumulation have inherent drawbacks, such as the production of harmful gases and toxic by-products. These limitations may be prudently addressed by employing green biological methods, such as biosorption and bioleaching. Therefore, this study was aimed at evaluating the biosorption and bioleaching potential of seven microbial cultures using E-waste (printed circuit board (PCB)) as a substrate under submerged culture conditions. The cut pieces of PCB were incubated with seven microbial cultures in liquid broth conditions in three replicates. Atomic absorption spectroscopy (AAS) analysis of the culture biomass and culture filtrates was performed to evaluate and screen the better-performing microbial cultures for biosorption and bioleaching potentials. The best four cultures were further evaluated through SEM, energy-dispersive X-ray spectroscopy (EDX), and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) studies to identify the possible culture that can be utilized for the biological decontamination of E-waste. The study revealed the highest and differential ability of Pleurotus florida and Pseudomonas spp. for biosorption and bioleaching of copper and iron. This can be attributed to bio-catalysis by the laccase enzyme. For both P. florida and Pseudomonas spp. on the 20th day of incubation, laccase exhibited higher specific activity (6.98 U/mg and 5.98 U/mg, respectively) than other microbial cultures. The biomass loaded with Cu2+ and Fe2+ ions after biosorption was used for the desorption process for recovery. The test cultures exhibited variable copper recovery efficiencies varying between 10.5 and 18.0%. Protein characterization through SDS-PAGE of four promising microbial cultures exhibited a higher number of bands in E-waste as compared with microbial cultures without E-waste. The surface topography studies of the E-waste substrate showed etching, as well as deposition of vegetative and spore cells on the surfaces of PCB cards. The EDX studies of the E-waste showed decreases in metal element content (% wt/% atom basis) on microbial treatment from the respective initial concentrations present in non-treated samples, which established the bioleaching phenomenon. Therefore, these microbial cultures can be utilized to develop a biological remediation method to manage E-waste.

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COPPER IMPROVES THE PRODUCTION OF LACCASE BY Pleurotus sajor-caju WITH ABILITY TO GROW ON EFFLUENTS OF THE CITRUS INDUSTRY

Cited by 6 publications

References 41 publications

Bioproduction of α‐terpineol and R‐(+)‐limonene derivatives by terpene‐tolerant ascomycete fungus as a potential contribution to the citrus value chain

Bioproduction of α‐terpineol and R‐(+)‐limonene derivatives by terpene‐tolerant ascomycete fungus as a potential contribution to the citrus value chain

Improvement of Laccase Production by Thielavia terrestris Co3Bag1. Enhancing the Bio-Catalytic Performance of the Native Thermophilic TtLacA via Immobilization in Copper Alginate Gel Beads

Biosorption and Bioleaching of Heavy Metals from Electronic Waste Varied with Microbial Genera

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