Optimization of laboratory cultivation conditions for the synthesis of antifungal metabolites by bacillus subtilis strains

Сидорова, Т. М.; Асатурова, А. М.; Homyak, A.I.; Жевнова, Н. А.; Штерншис, М. В.; Томашевич, Н. С.

doi:10.1016/j.sjbs.2020.05.002

Cited by 17 publications

(12 citation statements)

References 26 publications

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“…The maximal B. subtilis production was 1 × 10 10 CFU mL −1 in MFB treated by ultrasound and three enzymes. This yield was about 20 times that of untreated MFB and about eight times that of MFB treated by only ultrasonic or enzymatic methods, and it is compatible with the yields obtained in other studies 28,38 . The production yield of B. subtilis positively depended on the content of soluble components.…”

Section: Resultssupporting

confidence: 89%

“…Chemical treatments often use acidic/basic agents or organic solutions. The resulting extreme pH, high ionic strength, and/or organic solvent residuals are problematic for the fermentation of probiotics 28 . Physical methods, such as ultrafine grinding or ultrasonic treatment, 13,24 can release intracellular components through the destruction of the microbial cell wall by shear stress.…”

Section: Introductionmentioning

confidence: 99%

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Ultrasonic and enzymatic pretreatments of Monascus fermentation byproduct for a sustainable production of Bacillus subtilis

Zhang

Wang

et al. 2021

J Sci Food Agric

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BACKGROUND Monascus fermentation byproduct (MFB) is a biowaste generated after food colorants are extracted. Using MFB to produce probiotics (Bacillus subtilis) is a sustainable way for the entire production to be used as food or animal feed additives. However, due to the rigidity of the Monascus mycelium cell wall, B. subtilis cannot sufficiently utilize the nutrients in MFB, leading to low biomass production efficiency. We studied the effects of ultrasonic treatment, papain, β‐glucanase, and chitosanase, and their combinations on improving the levels of soluble components from MFB. The effects of these treatments on mycelium cell walls were visualized using scanning electron microscopy, and their influence on B. subtilis production was analyzed. RESULTS Ultrasonic treatment increased the soluble components by 210 g kg−1, including 50 g kg−1 protein and 120 g kg−1 carbohydrates. An enzyme mixture increased the soluble components by 160 g kg−1, including 30 g kg−1 protein and 90 g kg−1 carbohydrates. The combination of the two methods achieved the highest increase of soluble components (up to 400 g kg−1) leading to a maximum B. subtilis production of 1 × 1011 colony‐forming unit mL−1. This yield was about 20 times greater than that using untreated MFB and about eight times greater than treatments using only ultrasonic or enzymatic methods. CONCLUSION The productivity of B. subtilis production using MFB as the sole medium can be greatly improved by ultrasound or enzymes, which cause the release of intercellular components or cell wall components. © 2020 Society of Chemical Industry

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Section: Resultssupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Ultrasonic and enzymatic pretreatments of Monascus fermentation byproduct for a sustainable production of Bacillus subtilis

Zhang

Wang

et al. 2021

J Sci Food Agric

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“…The use of antagonistic bacteria such as Bacillus species, which synthesize antifungal metabolites, is an influential method to suppress the growth of fungal pathogens [ 8 , 9 ]. The application of antagonistic B. subtilis as a biological treatment against dermatophyte pathogens was elucidated by B. subtilis cell approaches [ 10 , 11 ].…”

Section: Discussionmentioning

confidence: 99%

“…An approach to biological treatment is a modern trend to reduce the usage of antifungal drugs in the manipulation of the fungal infection [ 7 ]. The application of biological chemicals synthesized by antagonistic bacteria, which can produce metabolites in an antifungal manner, was utilized to control the fungal growth [ 8 , 9 ]. B. subtilis is one kind of bacteria used as an antagonist to suppress the growth of fungi.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of cyclic lipopeptides obtained from Bacillus subtilis to inhibit the growth of Microsporum canis isolated from cats

Tunsagool

Ploypetch

Jaresitthikunchai

et al. 2021

Heliyon

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Background and aim Microsporum canis ( M. canis ) is a dermatophyte fungal pathogen that causes ringworms. Cats are considered to be a dominant reservoir host enabling M. canis transmission to humans. The concerns of dermatophyte resistance were raised among the usage of antifungal drugs to treat the ringworm. This study aimed to evaluate the fungal activity of cyclic lipopeptides (CLPs) obtained from Bacillus subtilis ( B. subtilis ) as an alternative method for the inhibition of M. canis growth. Materials and methods The culture plate of M. canis from confirmed cats with ringworm infection was provided. The purification of CLP extract, fengycin, iturin A, and surfactin was carried out from B. subtilis by preparative thin-layer chromatography (PTLC) coupled with solid-phase extraction (SPE) methods. Half-maximal effective concentration (EC 50 ) and agar well diffusion assays were performed to determine the efficacy of Bacillus CLP extract, fengycin, iturin A, and surfactin to inhibit the growth of M. canis isolated from cats. Results All purified Bacillus substances displayed antifungal activity to control the growth of M. canis when compared with 80% ethanol (control). EC 50 values for CLP extract, fengycin, iturin A, and surfactin were 0.23, 0.05, 0.17, and 0.08 mg/mL, respectively. In agar well diffusion assay, the ability of CLP extract, fengycin, iturin A, and surfactin on fungal inhibition had no statistically significant difference at 24 and 48 h after treatment (p < 0.05). However, CLP extract showed a statistically significant difference on M. canis inhibition at 62.21% followed by surfactin with 59.04% at 72 h after treatment. Conclusion In vitro , Bacillus CLPs revealed an inhibitory effect on M. canis growth which is a zoonotic pathogen that causes ringworms. This study suggests an alternative approach to control the growth of M. canis using substances obtained from B. subtilis as a biomedicine agent with antifungal activity.

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“…Molasses was used as a carbon source and corn extract was used as a nitrogen source (Asaturova et al, 2015). The B. velezensis BZR 336 g culture was cultivated at 25 C for 48 h, and the B. velezensis BZR 517 culture was cultivated at 30 C for 36 h. These parameters were obtained from a previous study (Sidorova et al, 2020).…”

Section: Bacillus Strains Used For Experimentsmentioning

confidence: 99%

Biological control of important fungal diseases of potato and raspberry by two Bacillus velezensis strains

Асатурова

Штерншис

Tsvetkova

et al. 2021

PeerJ

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Stem canker and black scurf caused by Rhizoctonia solani are the important diseases in potato, while spur blight caused by Didymella applanata is a major disease in red raspberry. In Western Siberia, both crops are grown predominantly in small-scale farming that requires maximal usage of biological products for plant protection instead of chemicals. We evaluated two promising Bacillus velezensis strains BZR 336 g and BZR 517 isolated in the south of Russia (45°1′N, 38°59′E) for their biological control potentials against the potato and red raspberry diseases under the more severe weather conditions of Western Siberia (55°1′N, 82°55′ E). We tested two techniques to apply biocontrol agents: (1) coating the seeds (potato tubers) and (2) spraying over the plants (raspberry canes). In each case, we estimated B. velezensis strains on two plant cultivars differed by the disease resistance. The degree of B. velezensis influence on disease incidence and severity depended on the bacterial strain, the protected plant, and its cultivar. We also demonstrated that two B. velezensis strains significantly stimulated plant growth of potato, which contributed to the plant productivity on both cultivars. The BZR 336 g strain affected the potato productivity more than the BZR 517 strain. Under the influence of both bacterial strains, raspberry yield was significantly higher compared to the control on the susceptible cultivar. These findings indicated that two southern B. velezensis strains had proved their efficacy as biological control agents in the control of the serious fungal infection of potato and raspberry plants under the more severe ecological conditions of Western Siberia. For the first time, we demonstrated B. velezensis strains potential for use as biological control agents against R. solani on potato, and against D. applanata on red raspberry.

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Optimization of laboratory cultivation conditions for the synthesis of antifungal metabolites by bacillus subtilis strains

Cited by 17 publications

References 26 publications

Ultrasonic and enzymatic pretreatments of Monascus fermentation byproduct for a sustainable production of Bacillus subtilis

Ultrasonic and enzymatic pretreatments of Monascus fermentation byproduct for a sustainable production of Bacillus subtilis

Efficacy of cyclic lipopeptides obtained from Bacillus subtilis to inhibit the growth of Microsporum canis isolated from cats

Biological control of important fungal diseases of potato and raspberry by two Bacillus velezensis strains

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