Optimization of horse chestnut (Aesculus hippocastanum L.) somatic embryo conversion

Troch, Veronique; Werbrouck, Stefaan; Geelen, Danny; Labeke, Marie-Christine Van

doi:10.1007/s11240-009-9544-8

Cited by 24 publications

(15 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PEG is a non-plasmolyzing osmoticum that induces hydric deficit; it has been used to stimulate somatic embryo maturation in several species, such as Hevea brasiliensis (Linossier et al 1997), Picea abies (Svobodová et al 1999), Glycine max (Walker and Parrott 2001), Aesculus hippocastanum (Troch et al 2009), and C. papaya (Mishra et al 2010;Heringer et al 2013;Vale et al 2014). The effects of PEG action include the modulation and regulation of genes (Stasolla et al 2003a, b) and proteins (Heringer et al 2013;Vale et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Morphological analyses and variation in carbohydrate content during the maturation of somatic embryos of Carica papaya

Vale

Reis

Passamani

et al. 2018

Physiol Mol Biol Plants

View full text Add to dashboard Cite

Efficient protocols for somatic embryogenesis of papaya ( L.) have great potential for selecting elite hybrid genotypes. Addition of polyethylene glycol (PEG), a nonplasmolyzing osmotic agent, to a maturation medium increases the production of somatic embryos in .. To study the effects of PEG on somatic embryogenesis of ., we analyzed somatic embryo development and carbohydrate profile changes during maturation treatments with PEG (6%) or without PEG (control). PEG treatment (6%) increased the number of normal mature somatic embryos followed by somatic plantlet production. In both control and PEG treatments, pro-embryogenic differentiation to the cotyledonary stage was observed and was significantly higher with PEG treatment. Histomorphological analysis of embryonic cultures with PEG revealed meristematic centers containing small isodiametric cells with dense cytoplasm and evident nuclei. Concomitant with the increase in the differentiation of somatic embryos in PEG cultures, there was an increase in the endogenous content of sucrose and starch, which appears to be related to a rising demand for energy, a key point in the conversion of . somatic embryos. The endogenous carbohydrate profile may be a valuable parameter for developing optimized protocols for the maturation of somatic embryos in papaya.

show abstract

Section: Introductionmentioning

confidence: 99%

Morphological analyses and variation in carbohydrate content during the maturation of somatic embryos of Carica papaya

Vale

Reis

Passamani

et al. 2018

Physiol Mol Biol Plants

View full text Add to dashboard Cite

show abstract

“…chilling and growth regulator inhibitors, have been ineffective (Rugini and Baldoni 2005). A common procedure for maturation of high-quality SE is the culture of embryogenic tissues in media with decreased osmotic water potential by increasing the concentration of sucrose, gelling agents or by supplementing non-plasmolysing osmoticum (Krajňáková et al 2009;Troch et al 2009). In this investigation, we explored an alternative approach, the use of cellulose acetate semi-permeable membranes for embryo maturation.…”

Section: Discussionmentioning

confidence: 99%

An efficient regeneration system via somatic embryogenesis in olive

Cerezo

Mercado

Pliego‐Alfaro

2011

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Olive is one of the most important oil crops in the Mediterranean area. Biotechnological improvement of this species is hampered by the recalcitrant nature of olive tissue regeneration in vitro. In this investigation, we have developed an efficient regeneration system for juvenile olive explants via somatic embryogenesis. Embryogenic cultures were obtained at a rate of 25% by culturing isolated radicles from mature seeds in a modified olive medium (OMc) containing 2.5 lM 6-(dimethylallylamino) purine (2iP) and 25 lM indole-3-butyric acid (IBA) over 3 weeks and later transferring to the same medium without 2iP and with a lower IBA concentration. Two different basal formulations, OMc and olive cyclic embryogenesis medium (ECO) [1/4 OM macroelements, 1/4 Murashige and Skoog (MS) microelements and 1/2 OM vitamins supplemented with 550 mg l -1 glutamine], were tested for embryogenic callus proliferation and maturation. The growth rate of embryogenic calli was similar in both media. However, the regeneration of mature embryos, achieved by culturing embryogenic masses in the same medium without hormones and supplemented with 1 g l -1 activated charcoal, was significantly higher when embryos were cultured in the ECO basal formulation. Pre-culturing embryogenic masses in liquid medium for up to 4 weeks did not affect subsequent callus proliferation in solid medium. The maturation rate of small globular somatic embryos, 1-3 mm size, obtained after filtering liquid cultures through a 3 9 3 mm mesh, was also similar to control embryos cultured in solid medium. To improve the maturation and germination rates, the effect of culturing globular somatic embryos on semi-permeable cellulose acetate membranes was also tested. Membrane treatments reduced the regeneration of mature embryos from 56.5% in the control treatment to 40.6% when the membrane was applied during the first half of the 8-week maturation phase and to 18% when the membrane was applied during last 4 weeks of the maturation period. However, membrane treatments significantly enhanced the conversion of mature embryos to plants, increasing the embryo conversion rate from 1.5% in the control to an average value of 37.8% in the membrane treatment. Cotyledonary embryos that were matured on the membranes showed lower values of water and solute potential than controls, indicating that this treatment exerted a controlled desiccation rate that enhanced the recovery of plants. Keywords In vitro plant regeneration Á Olea europaea Á Suspension culture Á Semi-permeable membrane Á Somatic embryo Á Water potential Abbreviations 2iP 6-(Dimethylallylamino) purine BA 6-Benzyladenine DKW Driver and Kuniyuki medium ECO Olive cyclic embryogenesis medium IBA Indole-3-butyric acid MS Murashige and Skoog OMc Modified olive medium SE Somatic embryo

show abstract

“…Tissue culture has been successfully used to produce horsechestnut plants from a variety of somatic and gametic sources including microspores (Radojević, 1978;Radojević, Marinkovic, & Jevremovic, 2000), anther filaments (Capuana, 2016;Jörgensen, 1989;Kiss, Heszky, Kiss, & Gyulai, 1992), embryos (Profumo, Gastaldo, Bevilacqua, & Carli, 1991;Troch, Werbrouck, Geelen, & Van Labeke, 2009), and leaf and stem explants (Dameri, Caffaro, Gastaldo, & Profumo, 1986;Gastaldo, Carli, & Profumo, 1994;Šedivá, Vlašínová, & Mertelík, 2013). Embryogenic tissues can also be cryopreserved for long-term storage (Jekkel, Gyulai, Kiss, Kiss, & Heszky, 1998;Lambardi, De Carlo, & Capuana, 2005; Wesley-Smith, Walters, Pammenter, & Berjak, 2001), overcoming the storage problems of recalcitrant seeds (Section 8.4) (Pence, 1990).…”

Section: Perennation: Reproductionmentioning

confidence: 99%

Biological Flora of the British Isles: Aesculus hippocastanum

et al. 2019

View full text Add to dashboard Cite

This account presents information on all aspects of the biology of Aesculus hippocastanum L. (horse‐chestnut) that are relevant to understanding its ecological characteristics and behaviour. The main topics are presented within the standard framework of the Biological Flora of the British Isles: distribution, habitat, communities, responses to biotic factors, responses to environment, structure and physiology, phenology, floral and seed characters, herbivores and disease, history and conservation. Aesculus hippocastanum is a large deciduous tree native to the Balkan Peninsula. Native populations are small (<10,000 trees total) and apparently in decline, but the tree has been widely planted in gardens and streets across Europe and other temperate areas from the 17th century onwards. It was voted the UK's favourite tree in a 2017 poll. As a British neophyte, it is occasionally naturalised in open wooded habitats. Horse‐chestnut is renowned for the beauty of its large (up to 30 cm long), upright panicles of white flowers, and for the large seeds (up to 42 g each) used in the formerly common children's game of “conkers.” More recently, the triterpene glycosides, extractable from various plant parts but especially the seeds, have been widely used in medicine. In much of Europe, horse‐chestnut is affected by chestnut bleeding canker (caused by Pseudomonas syringae pv. aesculi), the horse‐chestnut leaf miner Cameraria ohridella and the leaf blotch fungus Guignardia aesculi. The canker is likely to lead to death of <10% individuals, but seeds of plants infested with the leaf miner are 40%–50% smaller, which may affect long‐term establishment in non‐planted areas.

show abstract

Optimization of horse chestnut (Aesculus hippocastanum L.) somatic embryo conversion

Cited by 24 publications

References 36 publications

Morphological analyses and variation in carbohydrate content during the maturation of somatic embryos of Carica papaya

Morphological analyses and variation in carbohydrate content during the maturation of somatic embryos of Carica papaya

An efficient regeneration system via somatic embryogenesis in olive

Biological Flora of the British Isles: Aesculus hippocastanum

Contact Info

Product

Resources

About