Optimization of Extraction Techniques and RP-HPLC Analysis of Antidiabetic and Anticancer Drug Mangiferin from Roots of ‘Saptarangi’ (Salacia chinensis L.)

Abstract

Saptarangi (Salacia chinensis L.) is a chief source of mangiferin, which has antidiabetic and anticancer properties. Extraction using continuous shaking extraction (CSE), ultrasonic extraction (UE), microwave assisted extraction (MAE), and steam bath assisted extraction (SBAE) were evaluated for extraction of mangiferin (MF) from roots of S. chinensis. The extracts were analyzed by reverse phase-high performance liquid chromatography (RP-HPLC) and Fourier transform-infra red spectroscopy (FT-IR) analysis. Operational parameters such as different solvents and exposure period were also evaluated. N,N-Dimethyl formamide (DMF 30%, v/v) extracted higher quantity of MF compared to other solvents. The results shows that the extraction of MF by SBAE was more efficient followed by MAE, CSE, and UE methods. Maximum quantity of MF (391.80 ppm) was obtained by SBAE technique and DMF (30%) as a solvent system. SBAE is the easiest and best method for screening of MF from S. chinensis. Furthermore, use of FT-IR validates the RP-HPLC technique for identification and quantification.

Impact of Different Extraction Solvents on Bioactive Compounds and Antioxidant Capacity from the Root of Salacia chinensis L.

“…Previous studies have used methanol, petroleum ether, chloroform, ethanol, acetone, and water as the solvents for extracting bioactive compounds from S. chinensis for further analysis [4][5][6]. Although extraction solvents have been extensively studied in other plant materials, such as macadamia skin waste [7], S. chinensis fruit pulp [8], and basil leaf [9], none of the previous studies have compared the impact of different common solvents on the extraction efficiency of phenolic compounds from the S. chinensis root. Therefore, this study aimed to determine the impact of different common solvents 2 Journal of Food Quality (water, absolute methanol, ethanol, acetone, 50% methanol, 50% ethanol, and 50% acetone) on the extraction efficiency of bioactive compounds, as well as antioxidant capacity from the root of S. chinensis, in order to identify the most appropriate solvent for further extraction and isolation of bioactive compounds and antioxidant capacity from S. chinensis.…”

Optimization of ultrasonic‐assisted enzymatic extraction of β‐carotene from orange processing waste

“…The peak of 1,925.77 cm −1 indicates C=O stretch of esters, and the peak of 1,641.47 cm −1 is the sign of C=C aromatic stretching hydroxyl group (6). The peak of 1,384.57 cm −1 is related to stretching CH symmetric (Kamil et al, ), and the peak corresponding to 1,253.07 cm −1 represents the C‐C ether stretching bond (Chavan, Ghadage, Kshirsagar, & Kudale, ). The peak of 1,050.56 cm −1 shows the functional group of CH 3 stretching (Chavan et al, ), and the peak of 881.40 cm −1 is related to R‐CH=CH‐R (Konwarh, Pramanik, Kalita, Mahanta, & Karak, ).…”

“…The peak of 1,925.77 cm −1 indicates C=O stretch of esters, and the peak of 1,641.47 cm −1 is the sign of C=C aromatic stretching hydroxyl group FIGURE 8 FT-IR specta of optimal ultrasonic-enzymatic treatment using ethanol solvent (pectinase concentration of 0.40 % (w/w), ultrasonic time of 115 min and pH of 5.11) and maceration treatment using ethanol solvent (maceration time of 115 min) (6). The peak of 1,384.57 cm −1 is related to stretching CH symmetric (Kamil et al, 2011), and the peak corresponding to 1,253.07 cm −1 represents the C-C ether stretching bond (Chavan, Ghadage, Kshirsagar, & Kudale, 2015). The peak of 1,050.56 cm −1 shows the functional group of CH 3 stretching (Chavan et al, 2015), and the peak of 881.40 cm −1 is related to R-CH=CH-R (Konwarh, Pramanik, Kalita, Mahanta, & Karak, 2012).…”

“…The peak of 1,384.57 cm −1 is related to stretching CH symmetric (Kamil et al, 2011), and the peak corresponding to 1,253.07 cm −1 represents the C-C ether stretching bond (Chavan, Ghadage, Kshirsagar, & Kudale, 2015). The peak of 1,050.56 cm −1 shows the functional group of CH 3 stretching (Chavan et al, 2015), and the peak of 881.40 cm −1 is related to R-CH=CH-R (Konwarh, Pramanik, Kalita, Mahanta, & Karak, 2012). Finally, the peak of 637.20 cm −1 is related to the functional group of CH=CH (Sinha et al, 2012b).…”

Isolation and Maximisation of Extraction of Mangiferin from the Root of Salacia chinensis L.

“…and Salacia chinensis, have been used as traditional medicine for the prevention and treatment of numerous ailments [1]. S. chinensis in particular has been traditionally used for the treatment of arthritis, inflammation, diabetes, obesity, liver disorder and certain cancers [5,6], with mangiferin reported as one of the major bioactive component in the root of S. chinensis [5,7].…”