Isolation and Maximisation of Extraction of Mangiferin from the Root of Salacia chinensis L.

Abstract

Mangiferin has been reported to exhibit anti-viral, anti-cancer, anti-diabetic, immunomodulatory and hepatoprotective properties. This study aimed to develop an HPLC method to isolate mangiferin from Salacia chinensis L. root; investigate the impact of solvents on yield; optimise the ultrasound-assisted extraction (UAE) technique; and compare mangiferin yield with continuously shaking extraction (CSE) and decoction techniques. The results showed that mangiferin, with a purity of over 88%, could be achieved by HPLC using a mixture of solvent A (water: acetonitrile: orthophosphoric acid, 96.8:3:0.2 (v/v/v)) and solvent B (acetonitrile). Solvent type significantly affected the extraction yield of mangiferin, and a mixture of acetone and water gave the highest extraction yield, as compared to other solvents or mixtures. UAE conditions, such as ultrasonic power, temperature, time and concentration of acetone significantly affected the extraction of mangiferin. Optimal UAE conditions were at an ultrasonic power of 250 W, temperature of 50 °C, acetone concentration of 40% and extraction time of 60 min. These optimal conditions could extract approximately 92 mg, whereas CSE and decoction only extracted 89.20 mg and 58.71 mg of mangiferin, respectively, from 1 g of S. chinensis root. Therefore, these UAE conditions are recommended for the extraction of mangiferin from S. chinensis root for further utilisation.

Biochemical Constituent of Ginkgo biloba (Seed) 80% Methanol Extract Inhibits Cholinesterase Enzymes in Javanese Medaka (Oryzias javanicus) Model

“…The study revealed the highest extractable solids (15.6%) with absolute methanol followed by 50% ethanol, 50% methanol, and 50% acetone (14.3%, 12.3%, and 12.2%, resp.). Aqua solvent extracted half of extractable solids in comparison with absolute methanol, whereas 100% ethanol and 100% acetone only extracted 25% of extractable solids extracted by 100% methanol [ 25 ]. The result showed that recovery yields of crude semisolid extract prepared from S. chinensis root was significantly affected by the extraction solvents.…”

Xanthone C-glycosides isomers purified from Dryopteris ramosa (Hope) C. Chr. with bactericidal and cytotoxic prospects

“…Various parts of mango plant ( Mangifera indica ) are rich source of MF ( Iseda, 1957a , Iseda, 1957b , Ajila et al, 2010 ). Mangiferin attracted the interest of many researchers due to its antioxidant nature ( Ngo et al 2019 ). An isomer, IsoMF (4-C-β-D-glucopyranosyl-1, 3, 6, 7 - tetrahydroxy xanthone), is not very common and it has been isolated from the aerial parts of Anemarrhena asphodeloides ( Aritomi and Kawasaki, 1970 ) and Dryopteris ramosa ( Ishaque et al 2021 ).…”