Impact of Different Extraction Solvents on Bioactive Compounds and Antioxidant Capacity from the Root of Salacia chinensis L.

Abstract

This study aimed to study the impact of selected common organic solvents on extractable solids, phytochemical composition, and antioxidant capacity of S. chinensis. The results showed that the tested solvents played an important role in extraction of total solid and phytochemical composition as well as antioxidant capacity of S. chinensis. Acetone (50% v/v) was found to be the optimal extraction solvent for extractable solids (12.2%), phenolic compounds (60 mg GAE/g DW), flavonoids (100 mg CE/g DW), proanthocyanidins (47.4 mg CE/g DW), and saponins (754 mg EE/g DW) as well as antioxidant capacity (ABTS 334 mM TE/g DW, DPPH 470 mM TE/g DW, FRAP 347 mM TE/g DW, and CUPRAC 310 mM TE/g DW). The extract prepared from 50% acetone had high levels of bioactive compounds (TPC 555 mg GAE/g CRE, flavonoids 819 mg CE/g CRE, proanthocyanidins 392 mg CE/g CRE, and saponins 1,880 mg EE/g CRE) as well as antioxidant capacity (ABTS 414 mM TE/g, DPPH 407 mM TE/g, FRAP 320 mg TE/g, and CUPRAC 623 mM TE/g), thus further confirming that 50% acetone is the solvent of choice. Therefore, 50% acetone is recommended for extraction of phenolic compounds, their secondary metabolites, saponins, and antioxidant capacity from the root of S. chinensis for further isolation and utilisation.

Evaluation of Phytochemical Constituents and Antibacterial Activity of Chromolaena Odorata L. Leaf Extract against Selected Multidrug Resistant Bacteria Isolated from Wounds

“…1. This is in accordance with the findings of Ngo et al [28] who reported highest yield of Salacia chinensis methanol extract compared to other solvents tested. The difference recorded on the percentage yield may be due to the difference in plant used or as a result of the varied polarity of the tested solvents; methanol and ethanol solvents are less polar compared to water.…”

Study on the Chemical Composition, Enzyme Inhibition and Antioxidant Activity of Ziziphora taurica subsp. cleonioides

“…Results of the percentage yield and the quantification of flavonoid and phenolic compounds, indicated that the solvent used, is a critical parameter to consider, for the estimation of TFC and TPC. Ethyl acetate, methanol and water are among the solvents used for the extraction of secondary metabolites and especially for polyphenolic compounds [33,34]. However, the different capacity of each individual compound presented in an extract to dissolve in a solvent as well as the polarity of the extraction solvent and the extraction technique, influence the results.…”

Determination of the Phenolic Composition and Antioxidant Activity of Pear Extracts

“…The solvent of the extraction is one of the main factors in the prognosis of the qualitative and quantitative composition of the isolated phenolic compounds [27][28][29].…”