Optimization of extraction of phenolics from leaves of Ficus virens

Abstract

Abstract:In this research, the conditions for extraction of phenolics from leaves of Ficus virens were optimized using response surface methodology (RSM). The extraction abilities of phenolics (EAP) and flavonoids (EAF), the 2,2-diphenyl-1-pierylhydrazyl (DPPH) free-radical scavenging potential, and the ferric reducing/antioxidant power (FRAP) were used as quality indicators. The results of single-factor experiments showed that temperature, ethanol concentration, extraction time, and the number of extraction cycles were the main influencing variables, and these provided key information for the central composite design. The results of RSM fitted well to a second degree polynomial model and more than 98% of the variability was explained. The ideal extraction conditions for EAP, EAF, DPPH free-radical scavenging potential, and FRAP were obtained. Considering the four quality indicators overall, the ideal extraction conditions were 58% ethanol at 57 °C for 37 min with three extraction cycles. At the ideal extraction conditions, the values of EAP, EAF, DPPH free-radical scavenging potential, and FRAP were 5.72%, 3.09%, 58.88 mg ascorbic acid equivalent (AAE)/g dry weight (DW), and 15.86 mg AAE/g DW, respectively. In addition, linear correlations were observed between EAP, EAF, and antioxidant potential.

Exploration of avocado by-products as natural sources of bioactive compounds

“…Ultrasonic extraction was very effective since it decreased the processing time, reduced the cost of extraction, prevented thermal damage, and enhanced the amount of bioactive compounds [ 16 , 17 ]. Thus, the avocado peel and seed extracts were obtained using this technique and a solution containing ethanol/water (80/20, v/v), because this is a favorable solvent in the extraction of polar substances such as phenolic compounds, does not have toxic effects on humans, and is environmentally friendly [ 18 ].…”

What is the best ethanol‐water ratio for the extraction of antioxidants from rosemary? Impact of the solvent on yield, composition, and activity of the extracts

“…Crossing several cell compartments such as walls and membranes to get to the vacuole may be particularly difficult for pure ethanol compared to water:ethanol mixtures. Unlike these latters, ethanol may indeed denaturate proteins that will eventually form a physical barrier hindering the solvent access to vacuolar polyphenols .…”

Total Polyphenol and Flavonoid Content and Antioxidant Capacity of Some Varieties of Persea americana Peels Consumed in Cameroon

“…The total phenolic content of ethanol peel extracts for all varieties was significantly higher than those of hydroethanolic extracts and aqueous extracts. The highest polyphenol content recorded with ethanol as solvent might arise from the fact that phenolic compounds are known to be more soluble in organic solvents [ 27 ]. The key role played by the polarity of organic solvents in the solubility of phenolic compounds of plants was highlighted by several reports in the literature [ 28 – 30 ].…”