Optimization of erythritol production from glycerol by Yarrowia lipolytica Wratislavia K1

Musial, I; Marcinkiewicz, Marta; Cibis, Edmund; Rywińska, Anita; Juszczyk, Piotr; Rymowicz, Waldemar

doi:10.1016/j.copbio.2011.05.296

Cited by 3 publications

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“…With glycerol-rich substrates from industrial raw oil waste, the protein content in Y. lipolytica biomass was in the range of 45-50.1% [34]. With hydrophobic culture media such as raw rapeseed oil, the protein content of Y. lipolytica biomass was reported to be as high as 56.4% [35]. Therefore, there is a promised opportunity to use fatty fish hydrolysate as tuna by-product in microbial protein production.…”

Section: Nutritional Composition Of Yeast Biomass Cultured On Tuna He...mentioning

confidence: 99%

Investigation of the Yeast Yarrowia lipolytica Cultivation on Tuna Head Protein Hydrolysate

Ngoc Thi Minh Ta,

Nguyen Hong Ngan,

Pham Thi Lan

2023

Curr. Appl. Sci. Technol.

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Tuna (Thunnus spp.) is a significant commodity within the fishing industry. However, up to half of the total tuna weight ends up as waste, including tuna heads, which contain valuable nutrients such as protein, polyunsaturated fatty acids, enzymes, minerals, and trace elements. The purpose of this study was to investigate an environmentally sustainable approach for tuna head processing by yeast cultivation. The oleaginous yeast Yarrowia lipolytica was successfully cultured on tuna head protein hydrolysate and the effects of culture conditions including inoculum size, hydrolysate pH and incubation volume on nutrition utilization and on yeast growth were investigated. Y. lipolytica showed good biomass accumulation when cultured on tuna head protein hydrolysates. The yeast biomass increased with decreasing incubation volume and reached 8.6 g/L. The yeast also showed the ability to reduce up to 83.7% of hydrolysate lipid content while remaining 70.7% of nitrogen content. The culture conditions had more effect on lipid reduction than on nitrogen reduction and a good correlation between the specific growth rate and lipid reduction was observed. The yeast biomass was 64.0% in protein content and 9.6% in lipid content, without optimization. The investigation results demonstrated the potential improvement of tuna head utilization as well as the potential application of yeast biomass cultivation.

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Section: Nutritional Composition Of Yeast Biomass Cultured On Tuna He...mentioning

confidence: 99%

Investigation of the Yeast Yarrowia lipolytica Cultivation on Tuna Head Protein Hydrolysate

Ngoc Thi Minh Ta,

Nguyen Hong Ngan,

Pham Thi Lan

2023

Curr. Appl. Sci. Technol.

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“…For a long time, strategies for improving the yield and production rate of target products in the fermentation industry mainly include the breeding of yeast strains with high fermentation performance and the exploration of new fermentation processes (Koh et al, 2003;Musial et al, 2011). In the early work of our lab, a yeast strain Y-22 which can ferment glucose to erythritol was isolated from nature.…”

Section: Manymentioning

confidence: 99%

Transcriptomic Analysis of the Erythritol High-Yielding Mutant Strain Yarrowia lipolytica Y44

Ren¹,

Ban²,

Huang³

et al. 2021

American Journal of Biochemistry and Biotechnology

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RNA-seq technique was used to analyze the transcriptomics ofYarrowia lipolytica control strain Po1g, an erythritol-producing natural isolate Yarrowia lipolytica Y 22 and an erythritol high-yielding mutant strain Y44 derived from Y 22. Functional annotation of genes and classification of metabolic pathways were carried out to identify Differentially Expressed Genes (DEGs). These DEGs were classified into related metabolic pathways to explain the molecular mechanism of high yield of erythritol production. The results showed that the upregulated genes in erythritol-producing natural isolate Y 22 and erythritol-producing mutant strain Y 44 were mainly involved in pentose phosphate pathway, Tricarboxylic Acid Cycle (TCA) and malic acid cycle. The downregulated genes were mainly involved in amino acid synthesis and oxycarboxylic acid metabolism. The synergistic regulation of the above metabolic pathways can increase the input and reduce the output of erythrose-4-P (E-4-P), which is the precursor of erythritol, promoting erythritol production. In addition, compared with the natural isolate Y 22, the genes related to cell wall synthesis were down-regulated and the expression of transmembrane transporter protein was up-regulated in high-yield mutant strain Y 44. In this way, the permeability of yeast cells is enhanced and the synthesized erythritol can be quickly transported to the outside of the cell, which reduces the decomposition of erythritol and further promotes its yield.

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Glycerol as a promising substrate for Yarrowia lipolytica biotechnological applications

Rywińska

Juszczyk

Wojtatowicz

et al. 2013

Biomass and Bioenergy

169

119

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Optimization of erythritol production from glycerol by Yarrowia lipolytica Wratislavia K1

Cited by 3 publications

References 0 publications

Investigation of the Yeast Yarrowia lipolytica Cultivation on Tuna Head Protein Hydrolysate

Investigation of the Yeast Yarrowia lipolytica Cultivation on Tuna Head Protein Hydrolysate

Transcriptomic Analysis of the Erythritol High-Yielding Mutant Strain Yarrowia lipolytica Y44

Glycerol as a promising substrate for Yarrowia lipolytica biotechnological applications

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