Glycerol as a promising substrate for Yarrowia lipolytica biotechnological applications

Rywińska, Anita; Juszczyk, Piotr; Wojtatowicz, M.; Robak, Małgorzata; Lazar, Zbigniew; Tomaszewska, Ludwika; Rymowicz, Waldemar

doi:10.1016/j.biombioe.2012.11.021

Cited by 166 publications

(125 citation statements)

References 161 publications

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“…As reported before, in batch culture the same strain, Y. lipolytica MK1, was able to produce up to 82.2 g/L of erythritol from glycerol with yield of 0.55 g/g and productivity of 0.84 g/(L·h) (19). Erythritol production parameters obtained in this study in continuous cultivation were higher than those observed by Rywińska et al (11) in a batch culture on crude glycerol using the parental strain Y. lipolytica Wratislavia K1. The application of crude glycerol at 13.3 Hz and 0.6 min -1 resulted in erythritol concentration of 58.2 g/L, yield of 0.38 g/g and productivity of 0.78 g/(L·h).…”

Section: Eff Ect Of C:n Ratio On the Growth And Erythritol Productionsupporting

confidence: 49%

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An Effective Method of Continuous Production of Erythritol from Glycerol by Yarrowia lipolytica MK1

Rakicka

Mirończuk

Tomaszewska-Hetman

et al. 2017

Food Technol. Biotechnol.

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SummaryThis study demonstrates the potential applicability of the UV mutant Yarrowia lipolytica MK1 for the valorisation of glycerol and erythritol production in a chemostat culture. The aim of this research is to investigate the optimal C:N ratio in the feeding medium in order to enhance erythritol production. The highest erythritol concentration, at 113.1 g/L with a volumetric erythritol production rate of 1.1 g/(L·h) and a yield of 0.57 g/g, was obtained in the feeding medium with a C:N ratio of 80:1. Moreover, no residual glycerol was observed in the culture broth during cultivation. The chemical composition of the biomass was analysed. The contents of lysine and threonine in the biomass protein amino acid profi le were higher than those required by the FAO/WHO for fodder yeast.

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Section: Eff Ect Of C:n Ratio On the Growth And Erythritol Productionsupporting

confidence: 49%

“…Various factors aff ecting fermentation have been examined, e.g. diff erent cultivation systems, type of substrate, and addition of salt or supplementation with minerals (9,(11)(12)(13)(14)(15). Up to now, the batch, fed-batch or repeated--batch cultures have been tested for erythritol biosynthesis from glycerol (9,13).…”

Section: Introductionmentioning

confidence: 99%

An Effective Method of Continuous Production of Erythritol from Glycerol by Yarrowia lipolytica MK1

Rakicka

Mirończuk

Tomaszewska-Hetman

et al. 2017

Food Technol. Biotechnol.

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“…Glycerol use is not just limited to cryopreservation. This molecule has been utilized in a wide range of applications in the pharmaceutical and food industry [41][42][43] and more recently as a solvent for green chemistry. 44 While glycerol was the first cryoprotectant molecule to be discovered 45 , the molecular mechanisms by which it acts to prevent freezing damage still remain unclear.…”

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confidence: 99%

Low-Density Water Structure Observed in a Nanosegregated Cryoprotectant Solution at Low Temperatures from 285 to 238 K

2016

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Liquid water structure is defined by its molecular association through hydrogen bonding. Two different structures have been proposed for liquid water at low temperatures, low density liquid (LDL) and high density liquid (HDL) water. Here we demonstrate a platform which can be exploited to experimentally probe the structure of liquid water in equilibrium at temperatures down to 238 K. We make use of a cryoprotectant molecule, glycerol which when mixed with water lowers the freezing temperature of the solution non-monotonically with glycerol concentration. We use a combination of neutron diffraction and computational modeling to examine the structure of water in glycerolwater liquid mixtures at low temperatures from 285 K to 238 K. We confirm that the mixtures are nano-segregated into regions of glycerol-rich and water-rich clusters. We examine the water structure and reveal that at the temperatures studied here, water forms a low density water structure which is more tetrahedral than that at room temperature. We postulate that nano-segregation allows water to form a low density structure which is protected by an extensive and encapsulating glycerol interface.

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Section: Discussionmentioning

confidence: 99%

“…According to da Silva et al (2009), Fonseca et al (2009, glycerol is a biodegradable molecule by aerobic or anaerobic pathway, however because its alkalinity and impurities, it is necessary to neutralize and filter it, in order that microorganisms can use it as carbon source. Liu et al (2011) and Rywinska et al (2013) have reported alkali 12-16%, methyl esther 15-18%, methanol 8-12% and water 2-3% as glycerin impurities.During the kinetics performed in liquid medium with crude glycerol and lactoserum, the strains CG and LS had a typical microbial behaviour growth. In stationary growth phase (50 h), the quantity of biomass, in dry weight, for CG12, CG18 and CG101 strains was 1.51, 1.54, 1.06 g/l respectively, whereas LS1, LS8 and LS147 strains produced 1.31, 1.34 y 0.84 g/l respectively.…”

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confidence: 99%

Characterization of Bacteria Isolation of Bacteria from Pinyon Rhizosphere, Producing Biosurfactants from Agro-Industrial Waste

Reyes-López¹,

González²,

González

et al. 2016

Polish Journal of Microbiology

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A b s t r a c tTwo hundred and fifty bacterial strains were isolated from pinyon rhizosphere and screened for biosurfactants production. Among them, six bacterial strains were selected for their potential to produce biosurfactants using two low cost wastes, crude glycerol and lactoserum, as raw material. Both wastes were useful for producing biosurfactants because of their high content in fat and carbohydrates. The six strains were identified by 16S rDNA with an identity percentage higher than 95%, three strains belonged to Enterobacter sp., Pseudomonas aeruginosa, Bacillus pumilus and Rhizobium sp. All strains assayed were able to grow and showed halos around the colonies as evidence of biosurfactants production on Cetyl Trimethyl Ammonium Bromide agar with crude glycerol and lactoserum as substrate. In a mineral salt liquid medium enriched with both wastes, the biosurfactants were produced and collected from free cell medium after 72 h incubation. The biosurfactants produced reduced the surface tension from 69 to 30 mN/m with an emulsification index of diesel at approximately 60%. The results suggest that biosurfactants produced by rhizosphere bacteria from pinyon have promising environmental applications. 184the lack of knowledge about the interaction between microbes and pinyon roots.Additionally, agro-industrial organic wastes has recently been used as substrate to cultivate BS due to its low cost and high availability (Cassidy and Hudak, 2001;Kitamoto et al., 2002; Abbasi et al., 2012;. It is well known that wastes from palm oil, canola oil, soybean, glycerol and buttermilk, may be used to achieve an increase in production of about ten times in the mass of BS produced higher than that obtained when the production media contain glucose as the carbon source (Makkar and Cameotra, 2002). For instance, Ron and Rosenberg (2001) and Abbasi et al. (2012) demonstrated that BS can be produced by P. aeruginosa through fruit wastes, waste vegetable oil and foodindustry wastes as the substrate. In this context, agroindustrial wastes generated in Chiapas are low cost raw material, renewable and abundant throughout the year, with a total generation of about 28-140 thousand tons per year. These wastes include crops and processing residues of corn, coffee, cocoa, mango, banana, oil palm and buttermilk (SAGARPA, 2007; Valdéz-Vázquez et al., 2010). Likewise, huge amounts of glycerol were generated from the biodiesel fuel production in Chiapas during a 5 years operation period and, because this crude glycerol is expensive to purify for use in other industries, it remains without any disposal method and becoming a significant environmental problem, but potentially valuable as substrate to produce BS.Hence, the isolation of bacteria strains from pinyon rhizosphere capable of biosurfactant production by using four agro-industrial wastes as substrate is of interest in commercial production. In the present study, the presence of potential biosurfactant producer bacteria was determined by the formation of a complex with CTAB w...

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Glycerol as a promising substrate for Yarrowia lipolytica biotechnological applications

Cited by 166 publications

References 161 publications

An Effective Method of Continuous Production of Erythritol from Glycerol by Yarrowia lipolytica MK1

An Effective Method of Continuous Production of Erythritol from Glycerol by Yarrowia lipolytica MK1

Low-Density Water Structure Observed in a Nanosegregated Cryoprotectant Solution at Low Temperatures from 285 to 238 K

Characterization of Bacteria Isolation of Bacteria from Pinyon Rhizosphere, Producing Biosurfactants from Agro-Industrial Waste

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