An Effective Method of Continuous Production of Erythritol from Glycerol by Yarrowia lipolytica MK1

Rakicka, Magdalena; Mirończuk, Aleksandra M.; Tomaszewska-Hetman, Ludwika; Rywińska, Anita; Rymowicz, Waldemar

doi:10.17113/ftb.55.01.17.4812

Cited by 13 publications

(11 citation statements)

References 26 publications

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“…Yeast Y. lipolytica produces erythritol under high osmotic pressure to protect the cells against outflow of water. Production of erythritol by this yeast has been widely studied ( Rymowicz et al, 2009 ; Yang et al, 2014 ; Rakicka et al, 2017b ), but the assimilation pathway of erythritol remains a puzzle. Despite the fact that this yeast can assimilate this polyol as a carbon source, some of the wild-type strains cannot utilize this compound.…”

Section: Resultsmentioning

confidence: 99%

A Role of a Newly Identified Isomerase From Yarrowia lipolytica in Erythritol Catabolism

et al. 2018

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Erythritol is a natural sweetener produced by microorganisms as an osmoprotectant. It belongs to the group of polyols and it can be utilized by the oleaginous yeast Yarrowia lipolytica. Despite the recent identification of the transcription factor of erythritol utilization (EUF1), the metabolic pathway of erythritol catabolism remains unknown. In this study we identified a new gene, YALI0F01628g, involved in erythritol assimilation. In silico analysis showed that YALI0F01628g is a putative isomerase and it is localized in the same region as EUF1. qRT-PCR analysis of Y. lipolytica showed a significant increase in YALI0F01628g expression during growth on erythritol and after overexpression of EUF1. Moreover, the deletion strain ΔF01628 showed significantly impaired erythritol assimilation, whereas synthesis of erythritol remained unchanged. The results showed that YALI0F1628g is involved in erythritol assimilation; thus we named the gene EYI1. Moreover, we suggest the metabolic pathway of erythritol assimilation in yeast Y. lipolytica.

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Section: Resultsmentioning

confidence: 99%

A Role of a Newly Identified Isomerase From Yarrowia lipolytica in Erythritol Catabolism

et al. 2018

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“… Rakicka et al (2016) developed a chemostat culture process and obtained very promising results when they replaced pure glycerol with crude glycerol (103 vs. 81.9 g/L) as carbon source. Several other studies has been published on the production of erythritol from crude glycerol in recent years ( Mironczuk et al, 2016 ; Yang et al, 2016 ; Rakicka et al,2017a,b ; Da Silva et al, 2018 ; Rakicka-Pustulka et al, 2020 ).…”

Section: Expanding Substrate Scope and Bioprocess Engineeringmentioning

confidence: 99%

Recent Advances in Producing Sugar Alcohols and Functional Sugars by Engineering Yarrowia lipolytica

Abbasi

Liu

Zhi

et al. 2021

Front. Bioeng. Biotechnol.

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The sugar alcohols and functional sugars have wide applications in food, pharmaceutical, and chemical industries. However, the smaller quantities of natural occurring sugar alcohols and functional sugars restricted their applications. The enzymatic and whole-cell catalyst production is emerging as the predominant alternatives. The properties of Yarrowia lipolytica make it a promising sugar alcohol and functional sugar producer. However, there are still some issues to be resolved. As there exist reviews about the chemical structures, physicochemical properties, biological functions, applications, and biosynthesis of sugar alcohols and/or functional sugars in Y. lipolytica, this mini review will not only update the recent advances in enzymatic and microbial production of sugar alcohols (erythritol, D-threitol, and xylitol) and functional sugars (isomaltulose, trehalose, fructo-oligosaccharides, and galacto-oligosaccharides) by using recombinant Y. lipolytica but also focus on the studies of gene discovery, pathway engineering, expanding substrate scope, bioprocess engineering, and novel breeding methods to resolve the aforementioned issues.

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“…In those conditions, the polyol productivity was 1.1 g/L.h with a yield of 0.67 g/g [60]. A final erythritol titer of 82 g/L was reached after 90 h of culture in a step-wise fed-batch bioreactor culture with molasses (60 g/L) and crude glycerol (150 g/L) as carbon sources [61]. A chemostat culturing process was also developed for Y. lipolytica strain MK1 for the conversion of glycerol into erythritol.…”

Section: Crude Glycerolmentioning

confidence: 99%

“…A chemostat culturing process was also developed for Y. lipolytica strain MK1 for the conversion of glycerol into erythritol. For a C/N ratio of 80/1, erythritol concentration in the culture medium reached 113.1 g/L with a production rate of 1.1 g/L.h and yield of 0.57 g/g [61]. The chemostat operated at a dilution rate of 0.01 h -1 and lasted for 600 h. By developing a push and pull strategy that consisted of overexpressing the genes GUT1 (encoding glycerol kinase) and TKL1 (encoding transketolase), an erythritol productivity of 0.073 g/g DCW .h was obtained within 96 h using a biomass of 14.6 g DCW /L and an initial glycerol concentration of 150 g/L [62].…”

Section: Crude Glycerolmentioning

confidence: 99%

Organic Wastes as Feedstocks for Non-Conventional Yeast-Based Bioprocesses

2019

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Non-conventional yeasts are efficient cell factories for the synthesis of value-added compounds such as recombinant proteins, intracellular metabolites, and/or metabolic by-products. Most bioprocess, however, are still designed to use pure, ideal sugars, especially glucose. In the quest for the development of more sustainable processes amid concerns over the future availability of resources for the ever-growing global population, the utilization of organic wastes or industrial by-products as feedstocks to support cell growth is a crucial approach. Indeed, vast amounts of industrial and commercial waste simultaneously represent an environmental burden and an important reservoir for recyclable or reusable material. These alternative feedstocks can provide microbial cell factories with the required metabolic building blocks and energy to synthesize value-added compounds, further representing a potential means of reduction of process costs as well. This review highlights recent strategies in this regard, encompassing knowledge on catabolic pathways and metabolic engineering solutions developed to endow cells with the required metabolic capabilities, and the connection of these to the synthesis of value-added compounds. This review focuses primarily, but not exclusively, on Yarrowia lipolytica as a yeast cell factory, owing to its broad range of naturally metabolizable carbon sources, together with its popularity as a non-conventional yeast.

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An Effective Method of Continuous Production of Erythritol from Glycerol by Yarrowia lipolytica MK1

Cited by 13 publications

References 26 publications

A Role of a Newly Identified Isomerase From Yarrowia lipolytica in Erythritol Catabolism

A Role of a Newly Identified Isomerase From Yarrowia lipolytica in Erythritol Catabolism

Recent Advances in Producing Sugar Alcohols and Functional Sugars by Engineering Yarrowia lipolytica

Organic Wastes as Feedstocks for Non-Conventional Yeast-Based Bioprocesses

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