A Role of a Newly Identified Isomerase From Yarrowia lipolytica in Erythritol Catabolism

Mirończuk, Aleksandra M.; Biegalska, Anna; Zugaj, Karolina E.; Rzechonek, Dorota A.; Dobrowolski, Adam

doi:10.3389/fmicb.2018.01122

Cited by 19 publications

(19 citation statements)

References 32 publications

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“…Therefore to increase the intracellular effective dissolved oxygen concentration, the VHb gene was cloned under a hybrid promoter, UAS1B 16 -TEF [34]. Consequently the overexpression cassette excluding the bacterial backbone was transformed into Y. lipolytica strain AJD [9], resulting in the strain AJD pAD-VHb. Proper integration into the Y. lipolytica genome was verified by PCR.…”

Section: Resultsmentioning

confidence: 99%

“…Due to its high capability for synthesis and storage of fatty acids it is used as a model organism for lipid metabolism in eukaryotic cells [5, 6]. In addition, it can naturally produce organic acids [7, 8] or polyols [9, 10] and it is used as a host for heterologous protein production and non-native chemicals [11]. One of the unique features of Y. lipolytica is a capability for utilization of many unconventional carbon sources, such as alkanes, glycerol or fatty acids [12, 13].…”

Section: Introductionmentioning

confidence: 99%

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Heterologous overexpression of bacterial hemoglobin VHb improves erythritol biosynthesis by yeast Yarrowia lipolytica

et al. 2019

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Background Yarrowia lipolytica is an unconventional yeast with a huge industrial potential. Despite many advantages for biotechnological applications, it possesses enormous demand for oxygen, which is a bottleneck in large scale production. In this study a codon optimized bacterial hemoglobin from Vitreoscilla stercoraria (VHb) was overexpressed in Y. lipolytica for efficient growth and erythritol synthesis from glycerol in low-oxygen conditions. Erythritol is a natural sweetener produced by Y. lipolytica under high osmotic pressure and at low pH, and this process requires high oxygen demand. Results Under these conditions the VHb overexpressing strain showed mostly yeast-type cells resulting in 83% higher erythritol titer in shake-flask experiments. During a bioreactor study the engineered strain showed higher erythritol productivity (QERY = 0.38 g/l h) and yield (YERY = 0.37 g/g) in comparison to the control strain (QERY = 0.30 g/l h, YERY = 0.29 g/g). Moreover, low stirring during the fermentation process resulted in modest foam formation. Conclusions This study showed that overexpression of VHb in Y. lipolytica allows for dynamic growth and efficient production of a value-added product from a low-value substrate.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Heterologous overexpression of bacterial hemoglobin VHb improves erythritol biosynthesis by yeast Yarrowia lipolytica

et al. 2019

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“…Its disruption almost impairs the growth of Y. lipolytica on EOL used as a sole carbon source. All the genes involved in EOL catabolism were found located on chromosome F in the so called erythritol utilization cluster [103].…”

Section: Erythritolmentioning

confidence: 99%

Sugar Alcohols and Organic Acids Synthesis in Yarrowia lipolytica: Where Are We?

2020

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Sugar alcohols and organic acids that derive from the metabolism of certain microorganisms have a panoply of applications in agro-food, chemical and pharmaceutical industries. The main challenge in their production is to reach a productivity threshold that allow the process to be profitable. This relies on the construction of efficient cell factories by metabolic engineering and on the development of low-cost production processes by using industrial wastes or cheap and widely available raw materials as feedstock. The non-conventional yeast Yarrowia lipolytica has emerged recently as a potential producer of such metabolites owing its low nutritive requirements, its ability to grow at high cell densities in a bioreactor and ease of genome edition. This review will focus on current knowledge on the synthesis of the most important sugar alcohols and organic acids in Y. lipolytica.

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“…The popularity of erythritol is based on its low caloric value (0-0.2 kcal g -1 ) in comparison to sucrose (4 kcal g -1 ) while reaching approximately 60-80% of the sweetness level of sucrose [6], the low impact on insulin release due to its chemical structure (Wölnerhanssenet al, 2016), therefore being safe for diabetics (Noda K, Nakayama K, 1994), and antibacterial properties in relation to creation of dental plaque [9]. Initial reports suggested that erythritol is not metabolized in the human body but the study by Hootman et al indicates that a fraction of erythritol intake may be processed to erythronate.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of YALI0B07117g Encoding Erythrose Reductase Homolog Results in Enhanced Erythritol Synthesis From Glycerol by the Yeast Yarrowia Lipolytica.

Szczepańczyk

Rzechonek

Dobrowolski

et al. 2021

Preprint

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BackgroundPolyols are a group of sweet alcohols, frequently used as food additives. The constantly rising demand for polyols requires the application of new strategies to increase the production. Erythritol is synthesized by the yeast Yarrowia lipolytica under high osmotic pressure as an osmoprotectant. The metabolic pathway resulting in erythritol production remains partially unknown. However, the last reaction resulting in erythritol synthesis is conducted by an erythrose reductase (ER).ResultsThe Y. lipolytica strain was genetically modified to increase the erythritol yield and productivity, using glycerol as a sole carbon source. The modification focused on the ER homologue YALI0B07117g after the in silico analysis of the protein sequences of all reported ER homologues. Initial results in shake-flask experiments proved the influence of the gene YALI0B07117g in erythritol synthesis. Deletion of the gene resulted in 3-fold and 2-fold increased production of mannitol and arabitol, respectively. Overexpression of the native ER homologue gene showed a positive influence on erythritol production. Bath cultures were conducted and the obtained strain reached the yield of 0.4 g/g. The specific consumption rate (qs) increased from 5.83 g/g/L for the WT strain to 8.49 g/g/L for the engineered strain, while the productivity of erythritol increased from 0.28 g/L/h for the A101 strain to 0.41 g/L/h for the modified strain.ConclusionsOverexpression of the gene YALI0B07117g resulted in increased production of erythritol in the yeast Y. lipolytica. Disruption of the metabolic pathway by deletion of the gene results in higher production titers of mannitol and arabitol. Application of the research may prove positive for shortening the cultivation time due to the increased consumption rate of the substrate combined with increased parameters of erythritol synthesis.

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A Role of a Newly Identified Isomerase From Yarrowia lipolytica in Erythritol Catabolism

Cited by 19 publications

References 32 publications

Heterologous overexpression of bacterial hemoglobin VHb improves erythritol biosynthesis by yeast Yarrowia lipolytica

Heterologous overexpression of bacterial hemoglobin VHb improves erythritol biosynthesis by yeast Yarrowia lipolytica

Sugar Alcohols and Organic Acids Synthesis in Yarrowia lipolytica: Where Are We?

Overexpression of YALI0B07117g Encoding Erythrose Reductase Homolog Results in Enhanced Erythritol Synthesis From Glycerol by the Yeast Yarrowia Lipolytica.

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