Optimization of elecrospinning process of zein using central composite design

Miri, Mohammad Amin; Movaffagh, Jebrail; Najafi, Mohammad B Habibi; Najafi, Masoud Najaf; Ghorani, Behrouz; Koocheki, Arash

doi:10.1007/s12221-016-6064-0

Cited by 45 publications

(29 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The scaffolding conditions are improved by adjusting the electrospinning parameters, which include the needle measurement, the collector, the voltage, and the pumped polymer quantity. After optimization of conditions [24], bead-free fibers with a minimum diameter were obtained. To that end, the needle's distance to the collector was 20 cm, the voltage was 8 kV, and the polymer flow was 0.5 mL/h, and a blend of 8% PCL and 5% I. graveolens were found to be suitable.…”

Section: Resultsmentioning

confidence: 99%

Development of Inula graveolens (L.) Plant Extract Electrospun/Polycaprolactone Nanofibers: A Novel Material for Biomedical Application

et al. 2021

View full text Add to dashboard Cite

Recently, there has been a growing interest in research on nanofibrous scaffolds developed by electrospinning bioactive plant extracts. In this study, the extract material obtained from the medicinal plant Inula graveolens (L.) was loaded on polycaprolactone (PCL) electrospun polymeric nanofibers. The combined mixture was prepared by 5% of I. graveolens at 8% (PCL) concentration and electrospun under optimal conditions. The chemical analysis, morphology, and crystallization of polymeric nanofibers were carried out by (FT-IR) spectrometer, scanning electron microscopy (SEM), and XRD diffraction. Hydrophilicity was determined by a contact angle experiment. The strength was characterized, and the toxicity of scaffolds on the cell line of fibroblasts was finally investigated. The efficiency of nanofibers to enhance the proliferation of fibroblasts was evaluated in vitro using the optimal I. graveolens/PCL solutions. The results show that I. graveolens/PCL polymeric scaffolds exhibited dispersion in homogeneous nanofibers around 72 ± 963 nm in the ratio 70/30 (V:V), with no toxicity for cells, meaning that they can be used for biomedical applications.

show abstract

Section: Resultsmentioning

confidence: 99%

Development of Inula graveolens (L.) Plant Extract Electrospun/Polycaprolactone Nanofibers: A Novel Material for Biomedical Application

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The average fiber diameter was determined as 1.1 ± 0.3 µm at 25% RH and 0.7 ± 0.2 µm at 50% RH whereby the diameter of merged fibers was considered as the fiber diameter of one fiber. Despite fusion of fibers, the average fiber diameter of zein fibers electrospun at 50% RH was slightly lower than reported in the literature for zein fibers [ 23 , 28 ]. The smaller fiber diameter could be explained by a much lower flow rate used in comparison to the literature [ 30 ], where a decrease in flow rate has been shown to lead to a decreased fiber diameter.…”

Section: Resultsmentioning

confidence: 54%

“…It has been reported that the morphology and fiber diameter are strongly influenced by solvent and processing parameters, e.g., the polymer concentration, solvent factors, applied voltage, tip-target distance and flow rate, as well as ambient parameters [ 27 ]. All fiber mats were electrospun at 10 cm and 15 cm tip-to-target distance, but no significant change in the average fiber diameter and fiber morphology could be detected, which was also investigated and found by Miri et al [ 28 ]. Therefore, neat zein fiber mats were electrospun at 10 cm distance, whereas the blends were electrospun at 15 cm distance, which was the working distance optimized by Dippold et al [ 14 ].…”

Section: Resultsmentioning

confidence: 65%

Electrospun Zein Fibers Incorporating Poly(glycerol sebacate) for Soft Tissue Engineering

et al. 2018

View full text Add to dashboard Cite

For biomedical applications such as soft tissue engineering, plant proteins are becoming increasingly attractive. Zein, a class of prolamine proteins found in corn, offers excellent properties for application in the human body, but has inferior mechanical properties and lacks aqueous stability. In this study, electrospun scaffolds from neat zein and zein blended with prepolymer and mildly cross-linked poly(glycerol sebacate) (PGS) were fabricated. Less toxic solvents like acetic acid and ethanol were used. The morphological, physiochemical and degradation properties of the as-spun fiber mats were determined. Neat zein and zein-PGS fiber mats with high zein concentration (24 wt % and 27 wt %) showed defect-free microstructures. The average fiber diameter decreased with increasing PGS amount from 0.7 ± 0.2 µm to 0.09 ± 0.03 µm. The addition of PGS to zein resulted in a seven-fold increase in ultimate tensile strength and a four-fold increase in failure strain, whereas the Young’s Modulus did not change significantly. Degradation tests in phosphate buffered saline revealed the morphological instability of zein containing fiber mats in contact with aqueous media. Therefore, the fibers were in situ cross-linked with N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide (EDC)/N-Hydroxysuccinimide (NHS), which led to improved morphological stability in aqueous environment. The novel fibers have suitable properties for application in soft tissue engineering.

show abstract

“…Only a few studies reported the effects of processing factors or solvent types on the electrospun zein fibers [12,13]. However, only one comprehensive optimization study for electrospinning of zein has been reported, up to now [14]. This optimization study is different in the way of taking the effects of multiple variables acting simultaneously into account by using Taguchi's orthogonal design to produce nanofibers from zein.…”

Section: Electrospunmentioning

confidence: 99%

“…The mean diameters of these fibers range from 106 to 264 nm (Table 4). However, Selling et al and Miri et al produced nanofibers with larger diameters (above 400 nm) from the zein solution in HAc, probably due to the combined effect of higher voltage (above 20 kv) and higher zein concentrations used in their study [12,14].…”

Section: Morphologies Of Zein Nanofibersmentioning

confidence: 99%

Effects of Solvent Type and Process Parameters on Electrospinnability of Zein through Orthogonal Experimental Design

Horuz

Belibağlı

2018

View full text Add to dashboard Cite

Electrospinning of zein from its solution with a non-toxic solvent is not easy. It certainly requires understanding the effects of critical parameters during electrospinning. In this paper, the first aim was to understand how the morphology and diameters of fibers produced from zein solutions were affected by solvent type used in solution and process parameters (flow-rate, voltage, and distance) by using Taguchi's orthogonal design. For this purpose, the optimum levels of factors were determined as follows: 12 kV of voltage, 15 µL/min of flow rate, 10 cm of distance, and acetic acid as solvent in order to obtain the thinnest, bead-free nanofiber. Secondly, this combination was further validated by conducting a confirmatory experiment using five different zein concentrations to study the effect of concentration. The mean diameters of fibers with 24 % zein concentrations were found as similar to the optimum conditions estimated, proving the applicability of Taguchi's method for electrospinning optimization.

show abstract

Optimization of elecrospinning process of zein using central composite design

Cited by 45 publications

References 25 publications

Development of Inula graveolens (L.) Plant Extract Electrospun/Polycaprolactone Nanofibers: A Novel Material for Biomedical Application

Development of Inula graveolens (L.) Plant Extract Electrospun/Polycaprolactone Nanofibers: A Novel Material for Biomedical Application

Electrospun Zein Fibers Incorporating Poly(glycerol sebacate) for Soft Tissue Engineering

Effects of Solvent Type and Process Parameters on Electrospinnability of Zein through Orthogonal Experimental Design

Contact Info

Product

Resources

About