Optimization of DNA extraction of physic nut (Jatropha curcas) by selecting the appropriate leaf

Prayitno, Edi; Nuryandani, Einstivina

doi:10.13057/nusbiosci/n030101

Cited by 2 publications

(2 citation statements)

References 189 publications

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“…In addition, due to large size of genomic DNA, their DNA bands in agarose should be in the top of the agarose gel, on the same line with the largest IOP Publishing doi:10.1088/1755-1315/1115/1/012002 4 size of lambda marker. In accordance to opinion of Prayitno and Nuryadani [14], smaller DNA fragments move faster than larger DNA fragments and vice versa.…”

Section: Dna Extractionsupporting

confidence: 81%

Identification of the PLAG1 polymorphisms in Peranakan Ongole cattle population

Akmalputra

Sukaryo²,

Yanti³

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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Genetic factor plays a very important role in determining of cattle performance. A gene which is reported to be controlling cattle growth and body size is pleomorphic adenoma gene 1 (PLAG1). Therefore, the goal of this study was to identify PLAG1 polymorphisms in PO cattle population. A total of 43 unproductive PO cows from the slaughterhouse at Kebumen were used in this study. Blood samples were collected individually when the cattle were slaughtered. Furthermore, DNA genome was harvested from blood samples and used as DNA template for polymerase chain reaction (PCR) targeting g.48308 C>T, g.32212 (19 bp indel) and g.45233 T>C polymorphisms. g.48308 C>T and g.45233 T>C SNPs were genotyped using PCR-restriction fragment length polymorphism (PCR-RFLP) technique and a 19 bp indel of PLAG1 was directly genotyped by observing PCR products. Both allele and genotype frequencies were calculated to test Hardy-Weinberg equilibrium (HWE) status. The results showed that g.48308 C>T SNP and 19 bp indel varied. Three genotypes of the g.48308 C>T SNP namely CC, CT and TT were found in this study and two genotypes (DD and DI) of 19 bp indel were also identified. Moreover, heterozygous CT genotype frequency of g.48308 C>T SNP was 0.51 and T allele frequency was 0.60. In term of 19 bp indel, DD genotype frequency was 0.88 and D allele frequency was 0.94. In conclusion, two polymorphisms of the PLAG1 were identified in PO cattle population.

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Section: Dna Extractionsupporting

confidence: 81%

Identification of the PLAG1 polymorphisms in Peranakan Ongole cattle population

Akmalputra

Sukaryo²,

Yanti³

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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“…Current study succeeded in extracting genomic DNA of winged beans seeds (Fig. 1) though it was DNA contaminated by either RNA, phenol or protein (Tenriulo et al, 2001) appeared as smear or light band; a molecule of either destructed DNA or unidentified organic matter (Prayitno & Nuryandani, 2011). Alternatively, it caused by the isolated-genomic DNA was not in a good condition.…”

Section: Resultsmentioning

confidence: 86%

ANALYSIS OF ENCODING GENE FOR PALMITIC ACID CONTENT OF WINGED BEAN (Pshopocarpus tetragonolobus (L.) DC) SEEDS

Sasongko

Amurwanto

2021

Biology

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Winged bean (Psophocarpus tetragonolobus (L.) (DC.) contains moderate to high fat in its seeds including palmitic acid (C18:0). Of those three sampling areas in Indonesia, the Sumateranese winged bean seeds contains 60%; which is important raw source of various oleochemical industries. Palmitoyl ACP is one among those enzyme involves in palmitic acid biosynthesis pathway from fatty acid synthase. Information about encoding genes of ACP enzyme which involves in palmitic acid biosynthesis pathway, therefore, becomes a prerequisite and became the aim of this study. Current study designed three different specific primer for palmitic acids namely: A. palmitoyl-ACP, B. KAS III, and C. acc D. each of them has the following nucleotide sequences: A. forward primer (F) 5'-GTC GTG CAT CGG GGA AGA A -3' and reverse (R) 5'-TCG CTC CAT TTA GGC TGC AA-3'. B. KAS III (F) 5'-TGC CAG ACA CCA TCA CAA ACT-3' and (R) 5'-TGA CGC CAG CGA TTA CAG C-3' and C. acc D (F) 5'-GGG CGT GAT GGA GTT TG-3' and (R) 5'-AGG TTG GCC TCG TTC TG-3' which were tested on three different genotypes the short pod winged beans, medium length pods and long pods ones on a PCR machine. The PCR resulted in amplicons

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Optimization of DNA extraction of physic nut (Jatropha curcas) by selecting the appropriate leaf

Cited by 2 publications

References 189 publications

Identification of the PLAG1 polymorphisms in Peranakan Ongole cattle population

Identification of the PLAG1 polymorphisms in Peranakan Ongole cattle population

ANALYSIS OF ENCODING GENE FOR PALMITIC ACID CONTENT OF WINGED BEAN (Pshopocarpus tetragonolobus (L.) DC) SEEDS

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