ANALYSIS OF ENCODING GENE FOR PALMITIC ACID CONTENT OF WINGED BEAN (Pshopocarpus tetragonolobus (L.) DC) SEEDS

Abstract

Winged bean (Psophocarpus tetragonolobus (L.) (DC.) contains moderate to high fat in its seeds including palmitic acid (C18:0). Of those three sampling areas in Indonesia, the Sumateranese winged bean seeds contains 60%; which is important raw source of various oleochemical industries. Palmitoyl ACP is one among those enzyme involves in palmitic acid biosynthesis pathway from fatty acid synthase. Information about encoding genes of ACP enzyme which involves in palmitic acid biosynthesis pathway, therefore, becomes a prerequisite and became the aim of this study. Current study designed three different specific primer for palmitic acids namely: A. palmitoyl-ACP, B. KAS III, and C. acc D. each of them has the following nucleotide sequences: A. forward primer (F) 5'-GTC GTG CAT CGG GGA AGA A -3' and reverse (R) 5'-TCG CTC CAT TTA GGC TGC AA-3'. B. KAS III (F) 5'-TGC CAG ACA CCA TCA CAA ACT-3' and (R) 5'-TGA CGC CAG CGA TTA CAG C-3' and C. acc D (F) 5'-GGG CGT GAT GGA GTT TG-3' and (R) 5'-AGG TTG GCC TCG TTC TG-3' which were tested on three different genotypes the short pod winged beans, medium length pods and long pods ones on a PCR machine. The PCR resulted in amplicons