Optimization of cultural conditions for growth associated chromate reduction by Arthrobacter sp. SUK 1201 isolated from chromite mine overburden

Dey, Satarupa; Paul, A. K.

doi:10.1016/j.jhazmat.2012.01.078

Cited by 45 publications

(23 citation statements)

References 27 publications

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“…Microbial reduction of hexavalent chromium has attracted increased interest, as this process not only relieves the toxicity of chromium but also leads to the precipitation at near-neutral pH for subsequent physical removal [8]. Chromium resistant and reducing bacteria isolated from chromium polluted environments such as tannery effluent and chromite mining environments have played a key role in chromium bioremediation [9][10][11][12][13]. [12][13][14], were used in this study.…”

Section: Introductionmentioning

confidence: 99%

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Reduction of Hexavalent Chromium by Viable Cells of Chromium Resistant Bacteria Isolated from Chromite Mining Environment

2014

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Environmental contamination of hexavalent chromium [Cr(VI)] is of serious concern for its toxicity as well as mutagenic and carcinogenic effects. Bacterial chromate reduction is a cost-effective technology for detoxification as well as removal of Cr(VI) from polluted environment. Chromium resistant and reducing bacteria, belonging to Arthrobacter, Pseudomonas, and Corynebacterium isolated from chromite mine overburden and seepage samples of Orissa, India, were found to tolerate 12-18 mM Cr(VI) during growth. Viable cells of these isolates were also capable of growing and reducing 100 M Cr(VI) quite efficiently in Vogel Bonner (V.B.) broth under batch cultivation. Freshly grown cells of the most potent isolate, Arthrobacter SUK 1201, reduced 100 M Cr(VI) in 48 h. Reduction potential of SUK 1201 cells decreased with increase in Cr(VI) concentration but increased with increase in cell density and attained its maximum at 10 10 cells/mL. Chromate reducing efficiency of SUK 1201 was promoted in the presence of glucose and glycerol while the highest reduction was at pH 7.0 and 25 ∘ C. The reduction process was inhibited by divalent cations Ni, Co, and Cd, but not by Cu. Similarly, carbonyl cyanide m-chlorophenylhydrazone, N,N,-Di cyclohexyl carbodiimide, sodium azide, and sodium fluoride were inhibitory to chromate reduction, while 2,4 dinitrophenol promoted the process. Cells permeabilized by toluene increased the efficiency of Cr(VI) reduction and, thereby, indicate that Arthrobacter sp. SUK 1201, indigenous to chromite mining environment, could be used as an ideal tool for chromium bioremediation.

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Section: Introductionmentioning

confidence: 99%

“…Chromium resistant and reducing bacteria isolated from chromium polluted environments such as tannery effluent and chromite mining environments have played a key role in chromium bioremediation [9][10][11][12][13]. [12][13][14], were used in this study. Arthrobacter sp.…”

Section: Introductionmentioning

confidence: 99%

Reduction of Hexavalent Chromium by Viable Cells of Chromium Resistant Bacteria Isolated from Chromite Mining Environment

2014

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“…Similarly, Dey and Paul (2012) have reported that Cr(VI) by Arthrobacter sp. is accelerated by glycerol and glucose.…”

Section: Effect Of Different Carbon Sources On Cr(vi) Reductionmentioning

confidence: 71%

Leucobacter chromiireducens CRB2, a new strain with high Cr(VI) reduction potential isolated from tannery-contaminated soil (Fez, Morocco)

et al. 2015

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A new chromate-reducing bacterial strain was isolated from soil contaminated with tannery waste. Based on 16S rRNA gene sequence analyses, this strain was identified as Leucobacter chromiireducens CRB2. This bacterium had high multiresistance against heavy metals with a MIC of 700 mg/L Cr(VI) and was able to reduce Cr(VI) both aerobically and anaerobically. The optimum pH and temperature for Cr(VI) reduction were pH 8.0 and 30°C, respectively. Glycerol (10 mM) was the most efficient carbon source for Cr(VI) reduction by the strain followed by glucose. Moreover, Cr(VI) reduction by L. chromiireducens CRB2 was unaltered in the presence of other oxyanions. Bacterial cells immobilized in Na-alginate beads showed a high Cr(VI) reduction rates and exhibited an ability to repeatedly reduce Cr(VI). Therefore, immobilized cells were more effective than free cells in Cr(VI) reduction. Resting and permeabilized cell assays provided the better evidence of the presence of an enzymatic chromate reduction in L. chromiireducens CRB2. Assays conducted with cytosolic and particulate fractions of L. chromiireducens confirmed the role of cytosolic proteins in Cr(VI) reduction. Cr(VI) reduction by L. chromiireducens was mediated by a soluble enzyme contained in the cytoplasm after its adsorption on the cell surface. To the best of our knowledge, this is the first report studying parameters affecting Cr(VI) reduction and describing Cr(VI) removal mechanism by strain of L. chromiireducens.

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“…Previously it was also reported that the Cr can be a potential problematic pollutant commonly coupled with azo dyes in waste water released from dying industries (Yusuff and Sonibare, 2004). Few species were reported to have the ability to tolerate the very high levels of Cr (Kathiravan et al, 2010;Dey and Paul, 2012;Christl et al, 2012). However elevated levels of Cr negatively influence their survival and growth (Oleszkiewicz and Sharma, 1990).…”

Section: Discussionmentioning

confidence: 99%

“…This trend may be due to the synergistic effect of the strains in consortium form (Khehra et al, 2005;Saratale et al, 2009).Similarly another important finding of our study is that at higher concentrations of both Cr and azo dye reactive black-5, there was significant reduction in decolorization process occurred. The inhibiting effect on the decreasing the performance of bacteria might be due to the reason of toxic effect of these contaminants at increased concentration for bacterial cells (Dhal et al, 2010;Dey and Paul, 2012). Another important factor for this reduction can be the due to the production of morphological changes and oxidative stress on bacterial cell (Desai et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Effect of Chromium Forms on the Biodegradation of Reactive Black-5 Azo Dye by Psychrobacter and Klebsiella species

Khalid¹,

Arshad²,

Mahmood³

et al. 2015

IJAB

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To cite his paper: Khalid, A., J. Arshad, S. Mahmood, I. Aziz and M. Arshad, 2015 AbstractAzo dyes and chromium (Cr) are released in the wastewater of leather processing industry during tanning process. Several species of bacteria have shown ability to degrade azo dyes individually; however, presence of Cr ions in industrial effluents may affect the bacterial efficiency of removing azo dyes from waste water. In this study, biodegradation of reactive black-5 dye was studied in a mineral salts medium (MSM) liquid culture in the presence of Cr ions. Two bacterial strains Psychrobacter alimentarius KS23 and Klebsiella oxytoca N7 capable of degrading azo dyes were used individually as well as in a consortium for the degradation of azo dyes. Cr was applied in the form of K2Cr2O7, CrCl3, Cr2(SO4)3 at different concentrations (ranging from 0-250 mg L -1 ) of Cr. The results showed that presence of Cr in mineral salt medium significantly inhibited the azo dye decolorization. In the absence of Cr salts, complete decolorization of 100 mg dye L -1 was achieved by the bacterial strains in 8-12 h while up to 83% decolorization occurred in 72 h at 100 mg L -1 Cr. The rate of decolorization considerably decreased with increasing concentration of azo dye in the presence of 50 mg Cr L -1 medium. These findings imply that the existence of Cr in wastewater can significantly affect the bacterial efficiency in wastewater treatment systems to degrade azo dyes.

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Optimization of cultural conditions for growth associated chromate reduction by Arthrobacter sp. SUK 1201 isolated from chromite mine overburden

Cited by 45 publications

References 27 publications

Reduction of Hexavalent Chromium by Viable Cells of Chromium Resistant Bacteria Isolated from Chromite Mining Environment

Reduction of Hexavalent Chromium by Viable Cells of Chromium Resistant Bacteria Isolated from Chromite Mining Environment

Leucobacter chromiireducens CRB2, a new strain with high Cr(VI) reduction potential isolated from tannery-contaminated soil (Fez, Morocco)

Effect of Chromium Forms on the Biodegradation of Reactive Black-5 Azo Dye by Psychrobacter and Klebsiella species

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