Optimization of cello-oligosaccharides production by enzymatic hydrolysis of hydrothermally pretreated sugarcane straw using cellulolytic and oxidative enzymes

Oligosaccharides production by enzymatic hydrolysis of banana pseudostem pulp

“…This endo-glucanase enzyme was used, for example, in the saccharification of pre-treated rice straw supplemented with endo-β-glucosidase and β-glucosidase [34]. In the case of cello-OS production, combination of purified enzymes have been used by other authors [10,12], although the possibility of tuning commercially available cellulase mixtures to produce cello-OS has been proposed as a better option [11]; in any case, there are still some barriers that have hindered the scaling-up of the process.…”

“…In general, there is always a compromise between OS selectivity and OSY. For example, Barbosa et al [10] employed a combination of different endo-glucanases and with lactose and copper as additives, with hydrothermally pretreated sugarcane straw, and obtained almost a 100% of OS selectivity but just a 6.0% of OSY. Karnaouri et al [13] purposed a fine-tuning of the commercially available enzyme mixture Celluclast through pH modification, multi-stage hydrolysis with buffer exchange, and addition of β-glucosidase inhibitor.…”

“…There is a limited number of studies in the literature addressing cello-OS production from lignocellulosic materials, and these mainly focus on sugarcane straw [10], corncob residue [8], and forest biomass residues [11][12][13]. On the other hand, there are some references on the application of EH to the untreated and pre-treated banana pseudostem material [14][15][16][17][18][19], although these studies aim at the production of fermentable sugars, and no attention was paid to the possibility of obtaining OS, an aspect that is addressed in the present study.…”

Production of cello‐oligosaccharides through the biorefinery concept: A technical‐economic and life‐cycle assessment

“…The COS production section was based on the previous work published by this group (all the enzymatic reactions and upstream and downstream yields are stated in the previous manuscript). 13 Briefly, an optimal condition for COS production was established involving the synergism of the endoglucanases CcCel9M (GH9 from Clostridium cellulolyticum ATCC 35319) and CaCel (GH45 from Cryptopygus antarcticus) at 10 U/g of pretreated sugarcane straw; the lytic polysaccharide monooxygenase (LPMO) TrCel61A (AA9 from Trichoderma reesei) at 2 mg g −1 of pretreated sugarcane straw; the cellobiose dehydrogenase (CDH) NcCDHIIa (AA8 from Neurospora crassa OR74A) at 1 mg g −1 of pretreated sugarcane straw, and the additives copper (10 mM) and lactose (1 mM). All enzymes were heterologously expressed in Pichia pastoris under the vector pPICZ-α B (Thermo Fisher Scientific, Waltham, MA, USA.).…”

“…It was possible to reach 60.49 mg of COS/g of pretreated sugarcane straw after the optimization without glucose formation and, curiously, 87% of produced COS was cellopentaose. 13 Because of the cost of making cellopentaose, the current market is highly specialized and limited to analytical uses, costing USD 7.29/mg (Megazyme). If COS can be made more cheaply, they can enter new markets.…”