Oligosaccharides production by enzymatic hydrolysis of banana pseudostem pulp

Abstract

Banana production generates significant amounts of agricultural wastes, being fiber extraction one of the most relevant alternatives for their valorization. This process produces banana’s pseudostem pulp (BPP) as a byproduct, which shows an interesting composition for the biorefinery’s biochemical platform, with high polysaccharides (68%) and low lignin contents. This work deals with the enzymatic hydrolysis (EH) of raw and hydrothermally pre-treated BPP, focusing on the production of oligosaccharides (OS). Raw BPP hydrolysis with cellulase at different dosages rendered only 3.2% OS yields (OSY). Pectinase addition has not affected EH performance. On the other hand, EH of hydrothermally pre-treated BPP at 150 °C and 170 °C (P150 and P170) allowed to increase OSY up to 28% (P150, 1 FPU of cellulase/g dry biomass, 12 h), being 72% of the solubilized sugars in the form of cello-oligosaccharides. This last condition was subjected to a multi-stage EH strategy without improvements in OSY. An endo-glucanase was also tested, but obtained OSY were lower than cellulase results. Finally, obtained OS demonstrated to stimulate the growth of two Lactobacilli strains. The results show that BPP pre-treated under mild operational conditions is a good candidate for cello-oligosaccharides production by EH using 1 FPU/g DB of cellulase with a simple strategy. Graphical abstract

THE CULTURE OF CATFISH (Clarias sp.) FRY IN THE PEAT POND BASED ON PINEAPPLE (Ananas comosus L) TUBER AND BANANA (Musa sp.) STEM CRUDE EXTRACT WITH COMMERCIAL PROBIOTIC MIX

“…as an energy source (Mostafa, 2021). Diaz et al (2023) stated that banana stems contain cello-Fisheries Journal, 14(2), 1002-1010. http://doi.org/10.29303/jp.v14i2.884 Djauhari et al (2024) oligosaccharides which can be used as a potential source of prebiotics for future aquaculture. Furthermore, optimum growth and development of probiotics will help fish in digestive metabolism and nutrient absorption more efficiently by producing exoenzymes, namely digestive enzymes which will enrich the endoenzymes that fish naturally produce.…”

Production and Bioconversion Efficiency of Enzyme Membrane Bioreactors in the Synthesis of Valuable Products

“…The process typically consists of multiple steps, each serving a specific purpose. The first step is to select an appropriate base membrane material that possesses the desired properties such as high porosity, mechanical strength, chemical stability, and compatibility with the enzymatic reaction [ 18 , 19 ]. Common base membrane materials include polymeric membranes (e.g., PVDF, cellulose acetate, and polyethersulfone) and inorganic membranes (e.g., ceramic or glass membranes) [ 20 , 21 , 22 ].…”

Palm Kernel Cake Extracts Obtained from the Combination of Bacterial Fermentation and Enzymic Hydrolysis Promote Swine Small Intestine IPEC-J2 Cell Proliferation and Alleviate LPS-Induced Inflammation In Vitro

“…Treatment with enzymes and microorganisms can increase the nutritional value of PKC, primarily by reducing the amount of cellulose and mannan [ 2 ]. Numerous active substances are produced during the process, including volatile organic compounds (VOCs), such as ketones, aromatic hydrocarbons and short-chain fatty acids (SCFAs); microbial metabolites, such as amino acids, enzymes and antibiotics [ 3 , 4 ]; and oligosaccharides [ 5 ], thus promoting PKCs’ use in livestock, particularly in monogastric animals which cannot secrete various cellulases. Oligosaccharides are short-chain polymers with a low molecular weight, and they consist of 2–10 monosaccharide units [ 6 ] and present specific biological activities, such as aiding in the proliferation of Bifidobacteria, the regulation of gastrointestinal function, anti-inflammatory responses and the reduction in disease [ 7 , 8 ].…”