Optimization of Aspergillus niger lipase production by solid state fermentation of agro-industrial waste

Putri, Dwini Normayulisa; Khootama, Andy; Perdani, Meka Saima; Utami, Tania Surya; Hermansyah, Heri

doi:10.1016/j.egyr.2019.08.064

Cited by 42 publications

(17 citation statements)

References 17 publications

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“…A couple of studies supported the present research finding, which indicated olive oil as the most optimum inducer to aid the secretion of lipase from the local isolate. Improved lipase production from Aspergillus niger was observed when 5 % (v/v) and 1 % (v/v) olive oil were used as the inducer [22,23], which coincided with the outcomes from the present study. However, a study established coconut oil as the inducer and sole carbon source in promoting the highest lipase activity from Aspergillus tamarii.…”

Section: Optimization Of Types Of Inducersupporting

confidence: 91%

“…2, it was relatively straightforward that lipase's highest specific activity was observed when olive oil was used as the inducer and sole carbon source in this case, resulting in 8.89 a ± 0.05 mU/mg. Each oil has different lipid composition, which might account for the difference in the specific lipase activity when utilized as the inducer [23]. Two factors positively influence lipase production as an inducible enzyme, namely the type and concentration of lipid sources in the fermentation media, such as fatty acids [23].…”

Section: Optimization Of Types Of Inducermentioning

confidence: 99%

“…Each oil has different lipid composition, which might account for the difference in the specific lipase activity when utilized as the inducer [23]. Two factors positively influence lipase production as an inducible enzyme, namely the type and concentration of lipid sources in the fermentation media, such as fatty acids [23]. Olive oil emerged as the best inducer for lipase production in Rhodococcus strain UCC 009 since it contains high oleic acid content, which most lipases are specific towards, and low linoleic acid content, which have been reported to have a detrimental impact on lipase productivity [22].…”

Section: Optimization Of Types Of Inducermentioning

confidence: 99%

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Effect of inoculum size, inducer and metal ion on lipase production by Rhodococcus strain UCC 0009

et al. 2020

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Lipases are critical enzymes for industrial applications such as in the food and pharmaceutical fields. Therefore, the discovery of new lipases with enhanced characteristics are always encouraged. Thus, the present study explored the ability of a novel bacterial strain isolated from a tropical climate for lipase production. The optimization method using the one-variable-at-a-time approach was adopted to obtain increased production of lipase. The strain identified as Rhodococcus strain UCC 0009 was able to generate specific lipase activity of 11.67a ± 0.00 mU/mg at optimized conditions of 8 % (v/v) inoculum concentration, 1 % (v/v) olive oil as the inducer, and the addition of Ca2+ions. The specific lipase activity increased by 162 % when the optimization using a one-variable-ata-time approach was adopted compared to that of the non-optimized counterpart, signifying this experimental phase’s importance. The present study’s findings revealed the potential of utilizing Rhodococcus strain UCC 0009 as a green lipase producer for application in bioremediation and biotransformation at an industrial scale. Further study concentrating on enzyme characterization and improving culture conditions for conducive production of lipase via statistical optimization using response surface methodology (RSM) will be attempted to elucidate further the superiority of lipase obtained from local resources.

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Section: Optimization Of Types Of Inducersupporting

confidence: 91%

Section: Optimization Of Types Of Inducermentioning

confidence: 99%

Section: Optimization Of Types Of Inducermentioning

confidence: 99%

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Effect of inoculum size, inducer and metal ion on lipase production by Rhodococcus strain UCC 0009

et al. 2020

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“…Lipidic sources are fundamental inducers in lipase production and can also be used as the only carbon source in cultivation of lipase-producing microorganisms [43][44][45][46]. Many different vegetable oils are reported to be added in lipase production by fermentation, and fatty acid-derived surfactants, like Tween80, are popular enzyme inducers [45][46][47][48].…”

Section: Lipase Production From Soapstockmentioning

confidence: 99%

Enzymatic Methods for the Manipulation and Valorization of Soapstock from Vegetable Oil Refining Processes

Casali

Brenna

Parmeggiani

et al. 2021

Sustainable Chemistry

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The review will discuss the methods that have been optimized so far for the enzymatic hydrolysis of soapstock into enriched mixtures of free fatty acids, in order to offer a sustainable alternative to the procedure which is currently employed at the industrial level for converting soapstock into the by-product known as acid oil (or olein, i.e., free fatty acids removed from raw vegetable oil, dissolved in residual triglycerides). The further biocatalyzed manipulation of soapstock or of the corresponding acid oil for the production of biodiesel and fine chemicals (surfactants, plasticizers, and additives) will be described, with specific attention given to processes performed in continuous flow mode. The valorization of soapstock as carbon source in industrial lipase production will be also considered.

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“…Vários autores vêm propondo a utilização de resíduos agroindustriais como resíduos popularmente chamado de torta de dendê (Silveira et al, 2015), farelo de arroz (Putri et al, 2020), farelo de trigo (Dobrev et al, 2018) torta do óleo de canola (Rehman et al, 2019) dentre outros resíduos como indutores para a produção de enzimas.…”

Section: Introductionunclassified

Resíduos agroindustriais como substrato para a produção de lipases microbiana: uma revisão

Guedes

Santos

Ibiapina

et al. 2021

RSD

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O objetivo desse trabalho foi realizar uma pesquisa bibliográfica sobre a utilização de resíduos agroindústrias como substrato para produção de lipase microbiana, identificando os principais microrganismos, tempo de fermentação e a inclusão de indutores ao meio. Trinta trabalhos foram selecionados e formaram um banco de dados. Pesquisas no Brasil representaram 60% das publicações utilizadas, o resíduo (torta e borra) de dendê e soja foram os mais relatados representando 13,3%, os fungos filamentosos foram os mais estudados, o gênero Aspergillus foi o mais encontrado totalizando 36,6% e em especial a espécie A. niger, que foi estudada por oito pesquisadores. Em relação ao tempo, as fermentações variaram de 20 a 336 h, sendo que o tempo de 48 h foi utilizado por sete pesquisadores. Dentre as 30 publicações foi possível verificar que em 13 (43%) delas os autores não utilizaram nenhum tipo de indutor para suplementar o resíduo agroindustrial, porém a utilização de fontes lipídicas e principalmente de nitrogênio aumentaram significativamente a produção de lipase. A maioria das pesquisas foram conduzidas em FES (73,3%). Utilizar resíduos agroindustriais e suplementar o meio com indutores com preços mais baixos, além de potencializar a produção pode tornar o produto final mais barato.

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Optimization of Aspergillus niger lipase production by solid state fermentation of agro-industrial waste

Cited by 42 publications

References 17 publications

Effect of inoculum size, inducer and metal ion on lipase production by Rhodococcus strain UCC 0009

Effect of inoculum size, inducer and metal ion on lipase production by Rhodococcus strain UCC 0009

Enzymatic Methods for the Manipulation and Valorization of Soapstock from Vegetable Oil Refining Processes

Resíduos agroindustriais como substrato para a produção de lipases microbiana: uma revisão

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