Optimization of a freeze-drying process for the biocontrol agent Pseudomonas spp. and its influence on viability, storability and efficacy

Stephan, Dietrich A.; Silva, Ana-Paula Matos Da; Bisutti, Isabella

doi:10.1016/j.biocontrol.2015.12.004

Cited by 38 publications

(29 citation statements)

References 38 publications

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“…was as effective as fresh cells in two different plant-pathogen systems. 24 The efficacy of CPA-1 solid formulations against BBR in small-scale field trials has been demonstrated. Both tested solid formulations significantly reduced the disease at least as well as the liquid formulation of the BCA compared with the control.…”

Section: Discussionmentioning

confidence: 99%

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Novel film‐forming formulations of the biocontrol agent Candida sake CPA‐1: biocontrol efficacy and performance at field conditions in organic wine grapes

Carbó¹,

Usall²,

Marín

et al. 2018

Pest Management Science

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Section: Discussionmentioning

confidence: 99%

“…Spray‐dried Bacillus megaterium reduced rice sheath blight disease in the laboratory and greenhouse or freeze‐dried Pseudomonas spp. was as effective as fresh cells in two different plant‐pathogen systems …”

Section: Discussionmentioning

confidence: 99%

Novel film‐forming formulations of the biocontrol agent Candida sake CPA‐1: biocontrol efficacy and performance at field conditions in organic wine grapes

Carbó¹,

Usall²,

Marín

et al. 2018

Pest Management Science

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“…It has been evaluated for conservation of different strains of Pseudomonas fluorescens (Jean‐Noël et al ., ; Cabrefiga et al ., ; Bisutti et al ., ), Pseudomonas spp. (Stephan et al ., ) and strains of Pantoea agglomerans (Costa et al ., , ; Soto‐Muñoz et al ., ). Typically, the viability, e.g., of Pseudomonas spp.…”

Section: The Drying Processmentioning

confidence: 99%

Maintenance and assessment of cell viability in formulation of non‐sporulating bacterial inoculants

Berninger

López

Bejarano

et al. 2017

Microbial Biotechnology

156

106

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SummaryThe application of beneficial, plant‐associated microorganisms is a sustainable approach to improving crop performance in agriculture. However, microbial inoculants are often susceptible to prolonged periods of storage and deleterious environmental factors, which negatively impact their viability and ultimately limit efficacy in the field. This particularly concerns non‐sporulating bacteria. To overcome this challenge, the availability of protective formulations is crucial. Numerous parameters influence the viability of microbial cells, with drying procedures generally being among the most critical ones. Thus, technological advances to attenuate the desiccation stress imposed on living cells are key to successful formulation development. In this review, we discuss the core aspects important to consider when aiming at high cell viability of non‐sporulating bacteria to be applied as microbial inoculants in agriculture. We elaborate the suitability of commonly applied drying methods (freeze‐drying, vacuum‐drying, spray‐drying, fluidized bed‐drying, air‐drying) and potential measures to prevent cell damage from desiccation (externally applied protectants, stress pre‐conditioning, triggering of exopolysaccharide secretion, ‘helper’ strains). Furthermore, we point out methods for assessing bacterial viability, such as colony counting, spectrophotometry, microcalorimetry, flow cytometry and viability qPCR. Choosing appropriate technologies for maintenance of cell viability and evaluation thereof will render formulation development more efficient. This in turn will aid in utilizing the vast potential of promising, plant beneficial bacteria as sustainable alternatives to standard agrochemicals.

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“…was almost identical to that of the fresh cells. 37 In summary, we have demonstrated that a fluidised bed drying system is suitable for dehydrating C. sake cells because the dry formulation had satisfactory solubility, retained its viability for 12 months and was as effective as fresh cells against P. expansum on apples. In addition, it is easy to package, store and transport and is cost effective.…”

Section: Discussionmentioning

confidence: 82%

Dry formulations of the biocontrol agent Candida sakeCPA‐1 using fluidised bed drying to control the main postharvest diseases on fruits

2017

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Optimization of a freeze-drying process for the biocontrol agent Pseudomonas spp. and its influence on viability, storability and efficacy

Cited by 38 publications

References 38 publications

Novel film‐forming formulations of the biocontrol agent Candida sake CPA‐1: biocontrol efficacy and performance at field conditions in organic wine grapes

Novel film‐forming formulations of the biocontrol agent Candida sake CPA‐1: biocontrol efficacy and performance at field conditions in organic wine grapes

Maintenance and assessment of cell viability in formulation of non‐sporulating bacterial inoculants

Dry formulations of the biocontrol agent Candida sakeCPA‐1 using fluidised bed drying to control the main postharvest diseases on fruits

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