Novel film‐forming formulations of the biocontrol agent Candida sake CPA‐1: biocontrol efficacy and performance at field conditions in organic wine grapes

Abstract

The results of this work suggest that solid formulations would be a competitive alternative to conventional fungicides because they were easy to package and transport, and cell viability could be maintained for a long period of time. © 2018 Society of Chemical Industry.

Verifying the biocontrol activity of novel film‐forming formulations of Candida sake CPA‐1: resilience in relation to environmental factors, rainfall episodes, and control of Botrytis cinerea on different hosts

“…Despite the low resilience of C. sake CPA-1 at temperatures higher than 30 ∘ C, the efficacy of fluidized-bed spray-dried formulations was demonstrated under field conditions on grapes, where maximum temperatures were higher than 35 ∘ C. 19 In contrast, C. sake CPA-1 was isolated from apples after several months in a storage atmosphere 1 and the present study showed that the PS and MAL formulations maintained their viability after 30 days at 0 ∘ C regardless of the RH. It may therefore be possible to consider both preharvest and postharvest application as registered uses for the products.…”

“…The efficacy of both novel film‐forming formulations of CPA‐1 was demonstrated against B. cinerea on grapes in a laboratory‐based assay . The incidence and severity of gray mold and the severity of sour rot were significantly reduced under field conditions during two growing seasons . Botrytis cinerea produces significant losses in more than 200 crops worldwide with over 1400 plant species as possible hosts .…”

“…At the end of the optimization of a biocontrol product, experts should be able to answer many questions about advantages over other products, efficacy, survival under abiotic conditions, or market size . In connection with this, the development process of both film‐forming CPA‐1 formulations was followed stepwise to provide satisfactory answers to these questions …”

“…8 The incidence and severity of gray mold and the severity of sour rot were significantly reduced under field conditions during two growing seasons. 19 Botrytis cinerea produces significant losses in more than 200 crops worldwide 20 with over 1400 plant species as possible hosts. 21 Gray mold on grapes, berries, fruits, and tomatoes is one of the most common diseases produced by B. cinerea.…”

“…29 In connection with this, the development process of both film-forming CPA-1 formulations was followed stepwise to provide satisfactory answers to these questions. 8,17,19,24,30,31 The present study aimed to verify the potential of two recently developed C. sake CPA-1 formulations using a fluidized-bed spray-drying system, 8 and the specific objectives were as follows: (i) to examine CPA-1 resilience on wine grape berries under controlled conditions of temperature (0, 22, and 30 ∘ C) and relative humidity (RH) (40% and 85%); (ii) to determine the effect of simulated rainfall with different rain intensities and rain volumes on CPA-1 formulations applied to wine grapes; (iii) to evaluate the effect of different periods of cell establishment prior to rain wash; and (iv) to test the efficacy of CPA-1 film-forming formulations to control B. cinerea on the surface of different hosts, such as apples, pears and tomatoes.…”