Optimization, extraction, structure analysis and antioxidant properties of flavan-3-ol polymers: Proanthocyanidins isolated from Periploca angustifolia using surface response methodology

Diwani, Nouha; Fakhfakh, Jawhar; Athmouni, Khaled; Belhaj, Dalel; Feki, Abdelfattah El; Allouche, Noureddine; Ayadi, Habib; Bouaziz‐Ketata, Hanen

doi:10.1016/j.indcrop.2019.112040

Cited by 15 publications

(10 citation statements)

References 29 publications

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“…The 13 C-NMR was used to determine the properties of CTs, including PC, the PD extension unit and the heterocyclic ring structure. The distribution of NMR signals was based on the analysis of literature data [ 13 , 21 , 22 , 23 , 24 , 25 ]. The 13 C-NMR spectrum ( Figure 2 ) showed characteristic 13 C-peaks consistent with that of CTs with dominant PC units, and some PD units and PP units.…”

Section: Resultsmentioning

confidence: 99%

Structure Analysis and Study of Biological Activities of Condensed Tannins from Bruguiera gymnorhiza (L.) Lam and Their Effect on Fresh-Cut Lotus Roots

et al. 2021

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Bruguiera gymnorhiza (L.) Lam is a mangrove plant that spread in many parts of the world. Though mangrove plant polyphenols have been reported to exhibit many biological activities, little is known about mangrove plant tannins. To explore the application value of tannins from B. gymnorhiza, analyses on the structure and biological activity of condensed tannins (CTs) from Bruguiera gymnorhiza (L.) Lam were carried out. The results from 13C nuclear magnetic resonance (13C-NMR) and reversed-phase, high-performance liquid chromatography (RP-HPLC) showed that the CTs were dominated by procyanidins, with a small quantity of prodelphinidins and propelargonidins; and that the monomeric constituents of B. gymnorhiza tannins were catechin/epicatechin, gallocatechin/epigallocatechin and afzelechin/epiafzelechin. The CTs were reversible and mixed competitive inhibitors of tyrosinase and the 50% inhibiting concentration (IC50) was estimated to be 123.90 ± 0.140 μg/mL. The antioxidant activities of CTs from B. gymnorhiza leaves were evaluated, the IC50 for 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzo-thiazoline-6-sulfonic acid diammonium salt) (ABTS) scavenging activities were 88.81 ± 0.135 and 105.03 ± 0.130 μg/mL, respectively, and the ferric ion reducing antioxidant power (FRAP) value was 1052.27 ± 4.17 mgAAE/g. In addition, the results from fresh-keeping assays on fresh-cut lotus root reveal that CTs from B. gymnorhiza had excellent effects on inhibiting the activities of polyphenol oxidase (PPO) and peroxidase (POD), protecting fresh-cut lotus root from the oxidation of total phenolics and malondialdehyde (MDA) content and slowing the increase in total phenol content (TPC) at 4 °C during the whole storage period. Therefore, CTs showed good effects against the browning of fresh-cut lotus root. Together, these results suggested that B. gymnorhiza CTs are promising antibrowning agents for fresh-cut fruits.

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Section: Resultsmentioning

confidence: 99%

Structure Analysis and Study of Biological Activities of Condensed Tannins from Bruguiera gymnorhiza (L.) Lam and Their Effect on Fresh-Cut Lotus Roots

et al. 2021

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“…DPPH, NO scavenging activities, and TPC were taken as a measure of in vitro antioxidant profile of extract for optimization of extraction conditions using RSM (Diwani et al., 2020). In the current investigation, it has been observed that the variations in time and temperature of extraction showed distinct antioxidant activities.…”

Section: Resultsmentioning

confidence: 99%

Optimization of extraction conditions of Angelica archangelica extract and activity evaluation in experimental fibromyalgia

Kaur

Singh

Bhatti

2020

Journal of Food Science

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The plant Angelica archangelica, owing to its magnificent therapeutic effectiveness in folklore medicine system, has been regarded as an “angel plant.” The current investigation was aimed to optimize extraction conditions of A. archangelica roots and to investigate in vivo role of optimized extract in fibromyalgia. Plant material (dried roots) was subjected to methanol extraction at variable temperature (40 to 60 °C) and time (12 to 36 hr) conditions as per two‐factorial design strategy, and responses in terms of antioxidant activity were determined. The optimized extraction conditions were found to be temperature of 60 °C and time of 36 hr. HPLC fingerprinting indicated the presence of coumarins in extract. To induce fibromyalgia, the mice were administered reserpine at a dose of 0.5 mg/kg. Mice were orally treated with 100, 200, and 400 mg/kg extract, and magnitude of fibromyalgia was quantified. In comparison to reserpine group, the extract treatment attenuated pain as shown by significant increase in paw withdrawal threshold against mechanical stimuli (P < 0.05), improved motor ability indicated by increase in fall‐off time in inclined plane test (P < 0.05), improved locomotion indicated by increased square crossings in open field test (P < 0.05), and improved cognition as shown by significant reduction in time to reach platform in Morris water maze test and passive avoidance task test (P < 0.05). Extract treatment significantly halted reserpine‐induced rise in serum cytokine level (P < 0.05) and brain oxidative stress (P < 0.05). Angelica archangelica extract exerted its beneficial effects in fibromyalgia possibly through the attenuation of oxidative stress‐mediated inflammatory cascade. Practical Application Leads from natural products have become an integral part of drug designing processes and have high acceptability due to their better tolerance. The optimization of extraction conditions of plant yields better results and could reduce the processing time, thus increasing its industrial value.

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“…DPPH, ABTS, and reducing power assays are commonly classified as electron transfer (ET) methods, which are widely used to measure antioxidant properties of plant extracts. 23,24 DPPH free radical is extensively used for evaluating the scavenging activity of plant extracts. [25][26][27] This is due to the fact that DPPH free radical is sensitive enough to detect active compounds at low concentration.…”

Section: Antioxidant Property Of Extract Obtained By Solvothemal Extractionmentioning

confidence: 99%

Response surface methodology optimised solvothermal system enables an efficient extraction of echinacoside and oleuropein from Syringa pubescens Turcz

Chu

Yang

et al. 2021

Phytochemical Analysis

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Introduction: Syringa pubescens Turcz. was reported to be abundant in the Funiu Mountains of Henan Province and can be used to treat hepatitis and cirrhosis. In order to develop and utilise the resource, a fast and simple technique to extract bioactive compounds is needed.Objectives: Our aims were to provide an extraction technique of glycosides from S. pubescens and study the antioxidant activity of this material.Methods: Box-Behnken design (BBD) was employed with three factors at three levels. The process parameters such as ethanol concentration (X 1 ), temperature (X 2 ), and solvent-solid ratio (X 3 ) could significantly influence efficiency and yield of target compounds. High-performance liquid chromatography (HPLC) was used to determine the content of glycosides. DPPH (α,α-diphenyl-β-picrylhydrazyl), ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) and reducing power were used to evaluate the antioxidant activity of S. pubescens extract. Results:The optimal parameters for the maximal extraction yield were obtained with ethanol concentration of 68%, temperature of 89 C, solvent-solid ratio of 46 mL/g, and time of 20 min. The S. pubescens extract showed strong antioxidant properties in vitro. Conclusion:The findings indicated the potential application of solvothermal extraction method to extract bioactive compounds from S. pubescens Turcz. Furthermore, the S. pubescens extract could be used as an important resource of antioxidant activity.

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Optimization, extraction, structure analysis and antioxidant properties of flavan-3-ol polymers: Proanthocyanidins isolated from Periploca angustifolia using surface response methodology

Cited by 15 publications

References 29 publications

Structure Analysis and Study of Biological Activities of Condensed Tannins from Bruguiera gymnorhiza (L.) Lam and Their Effect on Fresh-Cut Lotus Roots

Structure Analysis and Study of Biological Activities of Condensed Tannins from Bruguiera gymnorhiza (L.) Lam and Their Effect on Fresh-Cut Lotus Roots

Optimization of extraction conditions of Angelica archangelica extract and activity evaluation in experimental fibromyalgia

Response surface methodology optimised solvothermal system enables an efficient extraction of echinacoside and oleuropein from Syringa pubescens Turcz

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