Optimization of extraction conditions of Angelica archangelica extract and activity evaluation in experimental fibromyalgia

Abstract

The plant Angelica archangelica, owing to its magnificent therapeutic effectiveness in folklore medicine system, has been regarded as an “angel plant.” The current investigation was aimed to optimize extraction conditions of A. archangelica roots and to investigate in vivo role of optimized extract in fibromyalgia. Plant material (dried roots) was subjected to methanol extraction at variable temperature (40 to 60 °C) and time (12 to 36 hr) conditions as per two‐factorial design strategy, and responses in terms of antioxidant activity were determined. The optimized extraction conditions were found to be temperature of 60 °C and time of 36 hr. HPLC fingerprinting indicated the presence of coumarins in extract. To induce fibromyalgia, the mice were administered reserpine at a dose of 0.5 mg/kg. Mice were orally treated with 100, 200, and 400 mg/kg extract, and magnitude of fibromyalgia was quantified. In comparison to reserpine group, the extract treatment attenuated pain as shown by significant increase in paw withdrawal threshold against mechanical stimuli (P < 0.05), improved motor ability indicated by increase in fall‐off time in inclined plane test (P < 0.05), improved locomotion indicated by increased square crossings in open field test (P < 0.05), and improved cognition as shown by significant reduction in time to reach platform in Morris water maze test and passive avoidance task test (P < 0.05). Extract treatment significantly halted reserpine‐induced rise in serum cytokine level (P < 0.05) and brain oxidative stress (P < 0.05). Angelica archangelica extract exerted its beneficial effects in fibromyalgia possibly through the attenuation of oxidative stress‐mediated inflammatory cascade. Practical Application Leads from natural products have become an integral part of drug designing processes and have high acceptability due to their better tolerance. The optimization of extraction conditions of plant yields better results and could reduce the processing time, thus increasing its industrial value.

Antioxidant and Antibacterial Activity of Extracts from Selected Plant Material

“…The selection of appropriate extraction conditions is also an important factor. Maximum DPPH scavenging activity was obtained when dry roots of A. archangelica were subjected to methanol extraction at a temperature of 60 • C and extraction time of 36 h [61]. With regard to the antioxidant activity determined for extracts from mulberry leaves collected in three regions in China, the species of plant material used and its origin had a strong influence.…”

Total Triterpenoid Extraction from Inonotus Obliquus Using Ionic Liquids and Separation of Potential Lactate Dehydrogenase Inhibitors via Ultrafiltration High-Speed Countercurrent Chromatography

“…When the concentration of the extract increased from 1.0 mol/L to 1.2 mol/L, the total triterpenoid content of I. obliquus decreased, and when the concentration of the extraction solution was too low, the ILs could not be fully utilized; hence, a concentration of the extraction solution range from 0.1 mol/L to 1.0 mol/L was selected. Furthermore, the total triterpenoid content of I. obliquus decreased slightly at 110 min and greatly after 120 and 150 min [24]. UAE was performed using a 750 W ultrasonic processor (BS-250; Kunshan Ultrasonic Instrument, Co., Ltd., Kunshan, China) at 80% amplitude [25] and 31 • C. Because ultrasonic waves increase the solvent temperature, ice coating was used to control the temperature during the extraction process.…”

Biological and Chemical Diversity of Angelica archangelica L.—Case Study of Essential Oil and Its Biological Activity

“…Furanocoumarins (such as archangelicin, bergapten, xanthotoxin, imperatorin, osthole, and others) [41,42] are constituents responsible for the antibacterial, [43] antiviral, [44] anti-inflammatory, [45] antitumor [46,47], hepatoprotective, [48] antidepressant [42], and other activities, as well as for phototoxicity [49]. For these reasons, the European Medicines Agency (EMA) commission pointed out risks associated with furanocoumarins in preparations of A. archangelica [50].…”