Optimisation of the cell cultivation methods in the embryonic stem cell test results in an increased differentiation potential of the cells into strong beating myocard cells

Thalidomide induced early gene expression perturbations indicative of human embryopathy in mouse embryonic stem cells

“…Induction of differentiation was achieved through embryoid body (EB) formation via hanging drop culture following a procedure adapted from De Smedt et al (2008). In brief, stem cells were thawed and a suspension was prepared at a concentration of 3.75 × 10 4 cells/ml in ESGRO Complete Basal Medium (EMD Millipore), which does not contain LIF, BMP-4, or GSK3b-I.…”

A roadmap for the development of alternative (non-animal) methods for systemic toxicity testing

“…This approach, which we termed "alert-driven testing" (Bremer et al, 2007a), bryotoxic potentials of even very closely related structures (de Jong et al, 2011). Optimizations of protocols were reported (De Smedt et al, 2008;Seiler et al, 2004;Seiler and Spielmann, 2011). In order to move towards transcriptomics read-outs, PCR was employed to monitor specific gene expression (Pellizzer et al, 2004).…”

The Role of Nanotechnology in Induced Pluripotent and Embryonic Stem Cells Research

“…272 273 It was reported that silica nanoparticles can inhibit differentiation to contractile myocardial cells in mouse EBs at concentrations at which cytotoxic effects were not observed in a test typically used to assess the embryo toxic potential of chemicals. [274][275][276][277][278][279][280] The potential for nanoparticle accumulation to transfer cumulatively to offspring should also be clearly addressed. 281 function over extended period of time; hence, the evaluation of potentially toxic effects should be performed immediately and for a long term as well.…”