Optimisation of process parameters for growth and bioactive metabolite produced by a salt-tolerant and alkaliphilic actinomycete, Streptomyces tanashiensis strain A2D

Singh, Laishram Shantikumar; Mazumder, S.; Bora, Tarun C.

doi:10.1016/j.mycmed.2009.07.006

Cited by 77 publications

(62 citation statements)

References 24 publications

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“…Highest significant antibiotic yield and maximum growth were obtained in a synthetic medium containing aspartic acid at 7% concentration as a nitrogen source. This result agreed with the finding of Singh et al (2009) who found that aspartic acid provided the highest biomass as well as antimicrobial agent by Streptomyces tanashiensis strain A2D.…”

Section: Saccharomonospora Viridissupporting

confidence: 93%

“…4) This suggests that this strain is an alkalophilic actinomycete. This result is comparable with some Streptomyces species recorded to produce antibiotics at alkaline pH (Basilio et al, 2003;Vasavada et al, 2006 andSingh et al, 2009). Thakur et al, (2009) reported that, the production of antimicrobial agent as well as growth by Streptomyces sp.…”

Section: Saccharomonospora Viridissupporting

confidence: 87%

“…Spores where then harvested and homogenized in 0.5% tween 20 (0.2 O.D.) 0.5 ml of this suspension was used as inoculum for optimization experiments (Singh et al, 2009). After the incubation period, the broth was filtered using Whatman No.1 filter paper.…”

Section: Basal Medium and Inoculum Preparationmentioning

confidence: 99%

“…In the term of its optimum temperature for growth, this organism appears to be thermophile in nature. The rapid growth rate of thermophilic actinomycetes and the reduction in contamination due to the elevated growth temperature make these organisms particularly useful for industrial fermentation (Tendler and Burkholder, 1960) rather than bioactive mesophiles that take more time (Sujatha et al, 2005 andSingh et al, 2009;Thakur et al 2009andArasu et al, 2009.…”

Section: Table1 Mean Diameters Of Inhibition Zones (Mmmentioning

confidence: 99%

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Production, Structural Elucidation and Biological Activities of Bioactive Metabolite of MarineSaccharomonospora viridi

2016

Egypt. J. Bot.

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wenty seven of one hundred thirty five marine actinomycete …….isolates were producers. Analysis of the 16S rRNA gene of the most potent bioactive isolate showed a strong similarity with Saccharomonospora viridis AHK190. For the production of antimicrobial metabolites, nutritional and environmental conditions have been optimized. Maximum antibiotic production was obtained when galactose was used as a sole carbon source at a concentration of 4.5% (w/v) in the presence of 7% (w/v) aspartic acid with salinity level 3% (w/v) and K 2 HPO 4 at 0.011%. Similarly initial medium pH of 8.0, incubation temperature of 55°C and incubation period of 72 hr were found to be the optimum. The chemical structure of this compound was elucidated on the basis of its spectroscopy data, elemental analysis and its molecular formula was found to be C 31 H 34 O 4 S 3 . The minimum inhibitory concentration of the pure compound against the tested bacteria was found to be in the range of 125-250 μg/ml and the minimum bacteriocidal concentration of the purified antibiotic was ranged between 250 and 500 μg/ml. For fungi, minimum inhibitory concentration was ranged between250-500µg/ml. The pure compound also found to have cytotoxic activity against human colon carcinoma with lethal concentration 50 of 24.2µg/ml.

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Section: Saccharomonospora Viridissupporting

confidence: 93%

Section: Saccharomonospora Viridissupporting

confidence: 87%

Section: Basal Medium and Inoculum Preparationmentioning

confidence: 99%

Section: Table1 Mean Diameters Of Inhibition Zones (Mmmentioning

confidence: 99%

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Production, Structural Elucidation and Biological Activities of Bioactive Metabolite of MarineSaccharomonospora viridi

2016

Egypt. J. Bot.

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“…The antimicrobial activity of this isolate would be tested against other microorganisms and on different culture media. In fact, it is now well established that the composition of the culture medium can affect the production of antibiotics by actinomycetes (Valan et al, 2008;Singh et al, 2009;Kuster and Williams, 1964;Tesic and Lukic, 1966;Waksman, 1967). For example, Lounès et al, (1995) showed that slowly assimilated carbon sources allow higher production of antibiotics whereas other studies showed that glucose causes repression of the formation of several aminoglycosides antibiotics produced by actinomycetes (streptomycin, kanamycin, neomycin and istamycine) by a repression of biosynthetic enzymes (Demain, 1989;Piepersberg and Distler, 1997).…”

Section: Detection Of Antimicrobial Activitymentioning

confidence: 99%

Isolation and molecular identification of antibiotic-producing actinomycetes from an old house in the medina of Fez, Morocco

Jihani¹,

Brodolin²,

Iraqui³

et al. 2012

Afr. J. Microbiol. Res.

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Modeling and optimization of antibacterial effect of lichen‐associated bacteria, Bacillus subtilis KSRLAB3 strain against marine fouling bacteria, Vibrio alginolyticus

Subbaiyan¹,

Ganesan²

2023

Biotech and App Biochem

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One of the most commonly occurring bacteria, Bacillus subtilis, can produce a wide variety of secondary metabolites. In this study, the antimicrobial effect of B. subtilis KSRLAB3 against Vibrio alginolyticus was optimized using the Plackett–Burman design (PBD) method, response surface methodology (RSM), and genetic algorithm (GA). Initially, the effects of carbon source, nitrogen source, NaCl concentration, pH, temperature, and incubation time on antimicrobial effects were studied. Among the carbon and nitrogen sources investigated, mannose and peptone elicited maximum antimicrobial effect. Using PBD, the most significant variables that influence the antimicrobial effect were identified, including incubation time, peptone concentration, and temperature. The optimum conditions required for attaining maximum antimicrobial effect was identified using the RSM‐GA hybrid method, and the optimum condition includes 49.999 h of incubation time, 4.39 g/L of peptone concentration, and 27.629°C of incubation temperature. The confirmatory experiments performed around the optimum condition showed a zone of inhibition of 35 ± 0.52 mm. Methanolic extract also proved the presence of antibacterial lipopeptide surfactin. Therefore, the RSM‐GA hybrid method was successfully used in this study to model the antimicrobial effect of B. subtilis KSRLAB3 against V. alginolyticus. The effective inhibition of V. alginolyticus can be investigated further for the development of antifouling coatings.

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Optimisation of process parameters for growth and bioactive metabolite produced by a salt-tolerant and alkaliphilic actinomycete, Streptomyces tanashiensis strain A2D

Cited by 77 publications

References 24 publications

Production, Structural Elucidation and Biological Activities of Bioactive Metabolite of MarineSaccharomonospora viridi

Production, Structural Elucidation and Biological Activities of Bioactive Metabolite of MarineSaccharomonospora viridi

Isolation and molecular identification of antibiotic-producing actinomycetes from an old house in the medina of Fez, Morocco

Modeling and optimization of antibacterial effect of lichen‐associated bacteria, Bacillus subtilis KSRLAB3 strain against marine fouling bacteria, Vibrio alginolyticus

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