Optimasi Proses Produksi Hidrolisat Protein Ikan (Hpi) dari Mujair ( Oreochromis Mossambicus ).

Ariyani, Farida; Saleh, Mohammad; Tazwir, Tazwir; Hak, Nurul

doi:10.15578/jpbkp.v9i5.462

Cited by 5 publications

(4 citation statements)

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“…In Indonesia, research on FPH production have been done mainly at laboratory scale using commercial enzymes, including papain enzyme (Ariyani, Saleh, Tazwir & Hak, 2003;Salamah, Nurhayati & Widadi, 2012;Nurhayati, Nurjanah and Casti, 2013;Annisa, Darmanto & Amalia, 2017), bromelain (Wijayanti, Romadhon & Rianingsih, 2018). The use of local microbial protease from thermophilic Bacillus sp.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Production of Fish Protein Hydrolysates in A Pilot Plant Scale

Martosuyono

Fawzya

Patantis

et al. 2019

Squalen Bull. Marine Fisheries Postharvest Biotech.

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Protease enzyme produced from Bacillus sp was employed to hydrolyze fish protein hydrolysates (FPH) under controlled conditions at a batch-pilot plant scale-process. Thirty kilograms of fish meat was mincedand mixed with 60 liters of water in 100 liters stainless steel vessel and 20,000 units of protease enzyme was added per kg of fish. Hydrolysis of fish was carried out at 55 oC for 6 hours. Multi stage of filtration were done to separate the FPH from unhydrolized fish residue. Mass balance were carried out to determine the rate of hydrolysis and yields. W ithout pH adjustment, 80% of substrate hydrolyzed could be achieved in 6 hour at 55 °C. Three kinds of products were recovered from the process, i.e solid residue, liquid FPH as filtration product, and spray dried FPH. Hydrolysis of 30 kg of fish meat substrate producing 1.7-2.0 kg of unhydrolyzed residue and 70 L of liquid FPH. Afterspray drying process of liquid FPH, 13 kg of FPH powder was recovered. The proximate and amino acid analysis of spray dried FPH showed that the FPH containing 20% of protein, rich in amino acids especially lysine and leucineand the residue still had 85,36% of protein (dry basis) that could be utilized for other purpose.

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Section: Introductionmentioning

confidence: 99%

Enzymatic Production of Fish Protein Hydrolysates in A Pilot Plant Scale

Martosuyono

Fawzya

Patantis

et al. 2019

Squalen Bull. Marine Fisheries Postharvest Biotech.

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show abstract

“…It can be used as an indicator that the higher NTT/NTB value, indicates that the fish is in post rigor phase. Perfect hydrolysis process will produce about 18-20 amino acids (Ariyani et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Production of Fish Hydrolysates Protein From Waste of Fish Carp (Cyprinus Carpio) by Enzymatic Hydrolysis

Saputra

Nurhayati

2016

ComTech

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Fish Protein Hydrolysates (FPH) is the mixed products of polypeptide, dipeptides, and amino acid. It can be produced from materials that contained of protein by acid reaction, base reaction or enzymatic hydrolysis. The objectives of this study were to study the production of FPH from fish carp meat at post rigor phase and viscera by enzymatic hydrolysis, to determine the specific activity of papain enzyme, and to determine the solubility of FPH. Capacity of fish hydrolyzing can be identified by analyzing the content of dissolved total nitrogen (NTT) compared with nitrogen total ingredient (NTB) in order to get the value of total soluble nitrogen/total nitrogen material (NTT/NTB). The hydrolysis processes were carried out in 0,26% (w/v) papain, 60 οC for 3 hours. The result showed that the specific activity of papain enzyme was about 3.28 U/mg. Solubility of FPH by comparing NTT/NTB was about 0.29% (fish meat) and 0.40% (fish viscera). Proximate test of protein content of fish meat was 18.34 ± 0.04 (g/100 g); while viscera was about 0.95±0.04 (g/100 g). The result indicated that product waste of fish carp had potential as a major of source of FPH.

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“…Enzim penghidrolisis yang biasa digunakan adalah enzim protease yang mana enzim ini dapat berasal dari hewan, tanaman maupun mikroba. Pepsin dan kemotripsin merupakan enzim protease yang berasal dari hewan, pronase dan pescalase 560 merupakan enzim protease yang berasal dari mikroba, sedangkan enzim protease yang berasal dari tanaman antara lain enzim bromelin, papain dan ficin (Ariyani et al, 2003). Papain merupakan salah satu enzim pemecah protein dari tanaman pepaya yang relatif mudah diperoleh.…”

Section: Pendahuluanunclassified

Pembuatan Hidrolisat Protein Hati Ayam Pedaging (Broiler) Dan Uji Aktivitas Antioksidannya

Wardi¹

2019

Scientia J. Far. Kes

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ABSTRAK Telah dilakukan penelitian tentang pembuatan hidrolisat protein hati ayam pedaging (broiler) dan uji aktivitas antioksidannya. Hidrolisat protein hati ayam (H) dibuat dengan cara enzimatis menggunakan variasi konsentrasi enzim papain sebesar 2%, 4%, 6% dan 8%. Uji aktivitas antioksidan dilakukan dengan menggunakan metoda DPPH. Pengujian aktivitas antioksidan diambil berdasarkan nilai persen inhibisi tertinggi dan dianalisis dengan SPSS uji ANOVA satu arah. Nilai persen inhibisi tertinggi terdapat pada hidrolisat hati ayam (H 6%). Hasil menunjukkan bahwa hidrolisat yang dihasilkan berbentuk cairan, berwarna kuning hingga kuning coklatan dan berbau khas. Nilai persentase derajat hidrolisis rata – rata berkisar antara 37,97 % sampai 68,82%. Nilai persentase kadar protein rata – rata berkisar antara 7,437 % sampai 12,348%. Hidrolisat protein hati ayam (H) dapat dibuat dengan menggunakan enzim papain dengan konsentrasi enzim 2%, 4%, 6% dan 8%. Aktivitas antioksidan (IC50) yang dihasilkan pada (H 6%) sebesar 876,913 ppm. Menurut klasifikasi aktivitas antioksidan yang dihasilkan dari hidrolisat protein hati ayam (H) sangat lemah, karena lebih dari 200 ppm.

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Optimasi Proses Produksi Hidrolisat Protein Ikan (Hpi) dari Mujair ( Oreochromis Mossambicus ).

Cited by 5 publications

References 0 publications

Enzymatic Production of Fish Protein Hydrolysates in A Pilot Plant Scale

Enzymatic Production of Fish Protein Hydrolysates in A Pilot Plant Scale

Production of Fish Hydrolysates Protein From Waste of Fish Carp (Cyprinus Carpio) by Enzymatic Hydrolysis

Pembuatan Hidrolisat Protein Hati Ayam Pedaging (Broiler) Dan Uji Aktivitas Antioksidannya

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