Optimal Production of β-Galactosidase from Lactobacillus fermentum for the Synthesis of Prebiotic Galactooligosaccharides (Gos)

Abstract

The enzyme β-galactosidase (β-gal) has extensively used for improvement of lactose intolerance condition. Present study, was designed to assess the potential of β-gal enzyme produced by Lactobacillus fermentum, a kefir isolate, as a biocatalyst for the manufacture of prebiotic galactooligosaccharides (GOS) from lactose. The efficiency of L. fermentum to produce β-gal of 4,254 u/ml was determined by permeabilizing the cells with solvents such as sodium dodecyl sulfate (SDS) and chloroform. Different parameters contributing β-gal production including reaction time, temperature, pH, carbohydrates, and substrate concentration on L. fermentum were studied and optimum β-gal activity was found to be 6,232.13 u/ml. It was observed that different experimental parameters for pH (7.0), temperature (35°C), and carbohydrates (galactose) were statistically significant (p<0.05). L. fermentum was found to produce GOS by transgalactosylation catalysed by β-gal during lactose hydrolysis which yielded di, tri, and tetra oligosaccharides, confirmed by TLC and HPLC. The culture showed β-gal activity, suggesting biotechnological applications and a promising organism for industrial β-gal production.

Cell permeabilization of Kluyveromyces and Saccharomyces species to obtain potential biocatalysts for lactose hydrolysis

“…The maximum beta-galactosidase activity of 1.220 U g -1 dry weight was obtained using permeabilized cells under the following optimized conditions, anionic detergent N-lauroyl sarcosine (N-LS) concentration of 1.5% w v -1 , solvent (water) volume of 1 mL (or 1.0 g wet weight mL -1 of N-LS solution), temperature of 25°C and incubation time of 20 min, (Yadav et al, 2015). Confirming that growing conditions can influence the microorganism metabolic characteristics, Mahadevaiah, Basavaiah, Parida, and Batra (2020) showed that the efficiency of Lactobacillus fermentum to produce beta-galactosidase of 4.254 U mL -1 was determined by permeabilizing the cells with solvents such as sodium dodecyl sulfate (SDS) and chloroform. Different parameters contributing beta-galactosidase production including reaction time, temperature, pH, carbohydrates, and substrate concentration on L. fermentum were studied and optimum enzyme activity was found to be 6.223,13 U mL -1 .…”

Production and Digestibility Studies of β-Galactosyl Xylitol Derivatives Using Heterogeneous Catalysts of LacA β-Galactosidase from Lactobacillus Plantarum WCFS1

“…Galactosyl derivatives of polyols (Gal-polyols) can be synthesized using the enzyme β-galactosidase. β-Galactosidase (EC 3.2.1.23) is an enzyme whose main function is to hydrolyze lactose to produce galactose and glucose, however under certain conditions it is also capable of catalyzing transgalactosylation reactions to produce different β-galactooligosaccharides (GOS) [14,15].…”