The latex agglutination immunoassay technique uses polymer colloids as carriers of adsorbed proteins to enhance the antigen-antibody reaction. The aim of the present work is to study the adsorption of Human Antirrabic Immunoglobulin (HA-IgG) on polystyrene latex (PS). The physical adsorption of HA-IgG on PS latex was investigated as a function of pH at 2 mM ionic strength. The amount of HA-IgG adsorbed onto PS latex greatly depends on pH; its value showed a maximum in the neighborhood of the IEP of HA-IgG. The electrophoretic method was applied to characterize latex particles. The influence of the amount of HA-IgG adsorbed (J(ads)) on the electrophoretic mobility and zeta-potential values was also studied.
A procedure was developed and validated for the determination of sugars by high performance liquid chromatography (HPLC). The system studied consisted of a Sugar-pak column operated continuously and with a methanol flow when not in use, as opposed to manufacturer's instructions, which recommend refrigeration of the column during the weekends when idle. This column is recommended for analysis of sugar products and process streams such as those of beet and sugarcane. Evaluations of this technique included a range of samples and procedure variables necessary to validate the effectiveness of the column. An excellent resolution and reproducibility was obtained among the sucrose, glucose, fructose and ethanol signals. The precision was within the accepted limits of less than 2% relative standard deviation.
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