Optical tweezers and cell biomechanics in macro- and nano-scale

Serafetinides, A. A.; Makropoulou, M.; Spyratou, Ellas

doi:10.1117/12.2013349

Cited by 4 publications

(2 citation statements)

References 43 publications

(55 reference statements)

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“…In this study, the size distribution of the vesicles was determined by optical tweezers using Einstein's diffusion coefficient equation utilizing the displacements of the individual particles inside the trap. This technique was demonstrated for the determination of trap stiffness (Gieseler et al,2021, Viana et al, 2007, Singer et al, 2000 and the calculation of cell biomechanics (Serafetinides et al,2013, Pesen et al, 2022, Zhang et al, 2019 before. To the author's knowledge, this is the first demonstration of the estimation of nanoliposomes' diameters by optical tweezers.…”

Section: Monolayer Liposome Measurementsmentioning

confidence: 99%

Determination of Protein Amount in Nanosized Synthetic Liposomes by Surface Effect Raman Spectroscopy (SERS)

PARLATAN

2023

Journal of Advanced Research in Natural and Applied Sciences

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Accurate characterization of synthetic liposomes is essential since they give information about the vesicular struc-tures in bodily fluids such as extracellular vesicles. The characterization tasks are generally the determination of the sizes of the liposomes and the profiling of the liposomes' content. Optical tweezers and Surface Enhanced Raman Spectroscopy (SERS) were used to profile the nanosized liposomes. The size distribution of the trapped liposomes (140 nm on average) was found by using Einstein's Brownian motion equation, consistent with the size distribution obtained from dynamic light scattering measurements. Besides, Gramicidin-encapsulated liposomes were measured using SERS, and statistically significant differentiation was found in Raman intensities between liposome populations with altering concentrations of proteins. This study uniquely measured size distributions of nano-sized liposomes with conventional optical tweezers (without plasmonics) and determined the chemical differ-ences between empty and protein encapsulated liposomes with high accuracy using Raman spectroscopy.

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Section: Monolayer Liposome Measurementsmentioning

confidence: 99%

Determination of Protein Amount in Nanosized Synthetic Liposomes by Surface Effect Raman Spectroscopy (SERS)

PARLATAN

2023

Journal of Advanced Research in Natural and Applied Sciences

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“…Alternatively, in our previous experimental studies, we used optical tweezers to calculate the bending rigidity and shear modulus 23,24 , by measuring sub-micrometer deformation of giant unilamellar liposomes under optical forces.…”

Section: Introductionmentioning

confidence: 99%

Characterization of new drug delivery nanosystems using atomic force microscopy

Spyratou

Mourelatou

Demetzos

et al. 2015

18th International School on Quantum Electronics: Laser Physics and Applications

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Liposomes are the most attractive lipid vesicles for targeted drug delivery in nanomedicine, behaving also as cell models in biophotonics research. The characterization of the micro-mechanical properties of drug carriers is an important issue and many analytical techniques are employed, as, for example, optical tweezers and atomic force microscopy. In this work, polyol hyperbranched polymers (HBPs) have been employed along with liposomes for the preparation of new chimeric advanced drug delivery nanosystems (Chi-aDDnSs). Aliphatic polyester HBPs with three different pseudogenerations G2, G3 and G4 with 16, 32, and 64 peripheral hydroxyl groups, respectively, have been incorporated in liposomal formulation. The atomic force microscopy (AFM) technique was used for the comparative study of the morphology and the mechanical properties of Chi-aDDnSs and conventional DDnS. The effects of both the HBPs architecture and the polyesters pseudogeneration number in the stability and the stiffness of chi-aDDnSs were examined. From the force-distance curves of AFM spectroscopy, the Young's modulus was calculated.

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AC electrokinetic immobilization of influenza virus

et al. 2022

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The use of alternating current (AC) electrokinetic forces, like dielectrophoresis and AC electroosmosis, as a simple and fast method to immobilize submicrometer objects onto nanoelectrode arrays is presented. Due to its medical relevance, the influenza virus is chosen as a model organism. One of the outstanding features is that the immobilization of viral material to the electrodes can be achieved permanently, allowing subsequent handling independently from the electrical setup. Thus, by using merely electric fields, we demonstrate that the need of prior chemical surface modification could become obsolete. The accumulation of viral material over time is observed by fluorescence microscopy. The influences of side effects like electrothermal fluid flow, causing a fluid motion above the electrodes and causing an intensity gradient within the electrode array, are discussed. Due to the improved resolution by combining fluorescence microscopy with deconvolution, it is shown that the viral material is mainly drawn to the electrode edge and to a lesser extent to the electrode surface.Finally, areas of application for this functionalization technique are presented.

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Optical tweezers and cell biomechanics in macro- and nano-scale

Cited by 4 publications

References 43 publications

Determination of Protein Amount in Nanosized Synthetic Liposomes by Surface Effect Raman Spectroscopy (SERS)

Determination of Protein Amount in Nanosized Synthetic Liposomes by Surface Effect Raman Spectroscopy (SERS)

Characterization of new drug delivery nanosystems using atomic force microscopy

AC electrokinetic immobilization of influenza virus

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