Optical Properties of Buffers and Cell Culture Media for Optofluidic and Sensing Applications

Hoang, Van Thuy; Stępniewski, Grzegorz; Czarnecka, Karolina; Kasztelanic, Rafał; Long, Van Cao; Xuan, Khoa Dinh; Shao, Liyang; Śmietana, Mateusz; Buczyński, Ryszard

doi:10.3390/app9061145

Cited by 53 publications

(32 citation statements)

References 18 publications

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“…Using the silica bead calibration plot (Fig. S1B), we obtained an estimated refractive index of 1.40 that agrees with measurement of n platelet ≈ 1.399 (51) with the assumption that n pbs ≈ 1.335 (52).…”

Section: Scattering Signal and Refractive Index Calibrationsupporting

confidence: 76%

Coherent Optical Scattering and Interferometry (COSI) for label-free multiparameter quantitative imaging of intra-thrombus stabilityin vitro

Zheng

Montague

Lim

et al. 2020

Preprint

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Although existing microfluidics in vitro assays recapitulate blood vessel microenvironment using surface-immobilized agonists under biofluidic flows, these assays do not quantify intra-thrombus mass and activities of adhesive platelets at agonist margin and uses fluorescence labeling, therefore limiting clinical translation potential. Here, we describe a real time label-free in vitro quantitative imaging flow assay called Coherent Optical Scattering and phase Interferometry (COSI) that evaluates both intra-thrombus and adhesive-only platelet dynamics using only changes in refractive index. By combining coherent optical scattering and optical interferometry, we evaluated and quantified both intra-thrombus mass with picogram accuracy and adhesive platelet-only events/dynamics with high spatial-temporal resolution (400 nm/s) under fluid shear stress using only changes in refractive index. Using oblique illumination, COSI provide a ~ 4 μm thin axial slice that quantifies the magnitude of physical of surface adhesive platelets (spreading, adhesion and consolidation) in a developing thrombus without labelling under fluid shear stress. We achieve real time visualization of recruitment of single platelet into thrombus and further correlate it to the developing mass of a thrombus. The adhesive platelet activity exhibit stabilized surface activity of around 2 μm/s and intra-thrombus mass exchange were balanced at around 1 picogram after treatment of a broad range metalloproteinase inhibitor (250 μM GM6001).

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Section: Scattering Signal and Refractive Index Calibrationsupporting

confidence: 76%

Coherent Optical Scattering and Interferometry (COSI) for label-free multiparameter quantitative imaging of intra-thrombus stabilityin vitro

Zheng

Montague

Lim

et al. 2020

Preprint

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“…The effect of the refractive index of the different media on the LSPR peak shift was investigated by Mie theory. Figure S1B in the supporting information shows the simulated extinction cross-section spectra of 15 nm AuNPs performed with reported refractive indexes (nr) of PBS (nr = 1.3348) and DMEM (nr = 1.3370) [ 45 ]. As can be seen in the figure, the simulations do not depict appreciably changes of the position of the maximum of the LSPR peak with respect of calculations performed in water (nr = 1.33), arguably due to similar refractive index values between the evaluated media.…”

Section: Resultsmentioning

confidence: 99%

Layer by Layer Assembled Chitosan-Coated Gold Nanoparticles for Enhanced siRNA Delivery and Silencing

Shaabani

Sharifiaghdam

Keersmaecker

et al. 2021

IJMS

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Delivery of small interfering RNA (siRNA) provides one of the most powerful strategies for downregulation of therapeutic targets. Despite the widely explored capabilities of this strategy, intracellular delivery is hindered by a lack of carriers that have high stability, low toxicity and high transfection efficiency. Here we propose a layer by layer (LBL) self-assembly method to fabricate chitosan-coated gold nanoparticles (CS-AuNPs) as a more stable and efficient siRNA delivery system. Direct reduction of HAuCl4 in the presence of chitosan led to the formation of positively charged CS-AuNPs, which were subsequently modified with a layer of siRNA cargo molecules and a final chitosan layer to protect the siRNA and to have a net positive charge for good interaction with cells. Cytotoxicity, uptake, and downregulation of enhanced Green Fluorescent Protein (eGFP) in H1299-eGFP lung epithelial cells indicated that LBL-CS-AuNPs provided excellent protection of siRNA against enzymatic degradation, ensured good uptake in cells by endocytosis, facilitated endosomal escape of siRNA, and improved the overall silencing effect in comparison with commercial transfection reagents Lipofectamine and jetPEI®. Therefore, this work shows that LBL assembled CS-AuNPs are promising nanocarriers for enhanced intracellular siRNA delivery and silencing.

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“…Further evaluations of the experimental data were conducted at a wavelength of 589 nm due to the availability of refractive indices, as the D-line of the sodium spectrum is usually used for determining the optical properties of materials. The absorption coefficient of PCL was presumed to be 0.0001 µm −1 , and the considered refractive indices of the used materials were 1.36 for ethanol, 1.33 for PBS, and 1.46 for PCL [23,35,36]. Further evaluations of the experimental data were conducted at a wavelength of 589 nm due to the availability of refractive indices, as the D-line of the sodium spectrum is usually used for determining the optical properties of materials.…”

Section: Methodsmentioning

confidence: 99%

Examining the Transmission of Visible Light through Electrospun Nanofibrous PCL Scaffolds for Corneal Tissue Engineering

Himmler

Schubert²,

Fuchsluger

2021

Nanomaterials

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The transparency of nanofibrous scaffolds is of highest interest for potential applications like corneal wound dressings in corneal tissue engineering. In this study, we provide a detailed analysis of light transmission through electrospun polycaprolactone (PCL) scaffolds. PCL scaffolds were produced via electrospinning, with fiber diameters in the range from (35 ± 13) nm to (167 ± 35) nm. Light transmission measurements were conducted using UV–vis spectroscopy in the range of visible light and analyzed with respect to the influence of scaffold thickness, fiber diameter, and surrounding medium. Contour plots were compiled for a straightforward access to light transmission values for arbitrary scaffold thicknesses. Depending on the fiber diameter, transmission values between 15% and 75% were observed for scaffold thicknesses of 10 µm. With a decreasing fiber diameter, light transmission could be improved, as well as with matching refractive indices of fiber material and medium. For corneal tissue engineering, scaffolds should be designed as thin as possible and fabricated from polymers with a matching refractive index to that of the human cornea. Concerning fiber diameter, smaller fiber diameters should be favored for maximizing graft transparency. Finally, a novel, semi-empirical formulation of light transmission through nanofibrous scaffolds is presented.

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Optical Properties of Buffers and Cell Culture Media for Optofluidic and Sensing Applications

Cited by 53 publications

References 18 publications

Coherent Optical Scattering and Interferometry (COSI) for label-free multiparameter quantitative imaging of intra-thrombus stabilityin vitro

Coherent Optical Scattering and Interferometry (COSI) for label-free multiparameter quantitative imaging of intra-thrombus stabilityin vitro

Layer by Layer Assembled Chitosan-Coated Gold Nanoparticles for Enhanced siRNA Delivery and Silencing

Examining the Transmission of Visible Light through Electrospun Nanofibrous PCL Scaffolds for Corneal Tissue Engineering

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