Optical probing and imaging of live cells using SERS labels

Kneipp, Janina; Kneipp, Harald; Rajadurai, Anpuchchelvi; Redmond, Robert W.; Kneipp, Katrin

doi:10.1002/jrs.2060

Cited by 146 publications

(132 citation statements)

References 38 publications

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“…However, the first intrinsic SERS experiments of cells were demonstrated later by Kneipp et al using 60 nm AuNPs with 830 nm excitation, requiring only 3-5 mW to obtain spectra with 1 second integration times, resulting in faster low-power imaging [282,283]. Since then, some experiments have been performed using the direct SERS approach, but as the SERS spectra often differ significantly from spotaneous Raman, they can be difficult to interpret when in the complex cell environment.…”

Section: Applicationsmentioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

255

189

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Raman spectroscopy is an increasingly popular technique in many areas including biology and medicine.It is based on Raman scattering, a phenomenon in which incident photons lose or gain energy via interactions with vibrating molecules in a sample. These energy shifts can be used to obtain information regarding molecular composition of the sample with very high accuracy. Applications of Raman spectroscopy in the life sciences have included quantification of biomolecules, hyperspectral molecular imaging of cells and tissue, medical diagnosis, and others. This review briefly presents the physical origin of Raman scattering explaining the key classical and quantum mechanical concepts. Variations of the Raman effect will also be considered, including resonance, coherent, and enhanced Raman scattering. We discuss the molecular origins of prominent bands often found in the Raman spectra of biological samples. Finally, we examine several variations of Raman spectroscopy techniques in practice, looking at their applications, strengths, and challenges. This review is intended to be a starting resource for scientists new to Raman spectroscopy, providing theoretical background and practical examples as the foundation for further study and exploration.

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Section: Applicationsmentioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

255

189

View full text Add to dashboard Cite

show abstract

“…Very recent, cell Raman imaging has been reported 36 using simple nanoaggregates like crystal violet or rose Bengal dye attached to gold nanoparticles, without any other capture agents like mercaptoacetic acid 1 and using the 830 nm diode laser excitation. When introduced into the cellular cavity, Raman scattering signal of the reporter indicates the label localization into the cell compartment and simultaneously SERS in the local optical field of the decorated nanoparticles probes the chemical composition in their close vicinity.…”

Section: Resultsmentioning

confidence: 99%

Double Amino Functionalized Ag Nanoparticles as SERS Tags in Raman Diagnostic

Pinzaru¹,

Fălămaș²,

Dehelean³

et al. 2013

Croat. Chem. Acta

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Abstract. Surface enhanced Raman scattering (SERS) effect is currently exploited as the basis of a new type of optical labels for in vivo investigation of the tissues, especially for early medical diagnostic. Silver colloidal nanoparticles decorated with chemisorbed cresyl violet molecular species could act as hybrid SERS labels. Their Raman scattering properties have been characterized here using different SERS techniques, and probing different excitation wavelengths, even in the near infrared. Obtaining FT-SERS signal of cresyl violet is of particular importance for applying FT-Raman spectroscopy to the tissues or cells, in providing sensitive information in the close vicinity of the SERS label incubated into the biological sample. Furthermore, upon chemisorption on the silver nanoparticles, cresyl violet molecular orientation provided both amino functional groups free of interaction with the silver, resulting double aminofunctionalized Ag nanoparticles suitable for DNA tagging. SERS tests of melanoma induced in mouse using CV-Ag SERS label suggested the possibility to setup the tissue labeling procedure for skin cancer monitoring.(doi: 10.5562/cca2067)

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“…The use of Surface Enhanced Raman Scattering from gold nanoparticles and nanoaggregates to probe the environment of the subcellular compartments through which they are trafficked has been demonstrated [124,125]. This approach also demonstrated the use of molecular labelled nanoparticles as more specific probes of the local environment [126][127][128]. However, the uptake rates and mechanisms as well as the subsequent trafficking may be specific to the nanoparticle type, size and surface chemistry.…”

Section: The Kinetics Concept: Timescales Of Interaction Of Nanopartimentioning

confidence: 99%

The bio-nano-interface in predicting nanoparticle fate and behaviour in living organisms: towards grouping and categorising nanomaterials and ensuring nanosafety by design

et al. 2013

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Abstract:In biological media, nanoparticles acquire a coating of biomolecules (proteins, lipids, polysaccharides) from their surroundings, which reduces their surface energy and confers a biological identity to the particles. This adsorbed layer is the interface between the nanomaterial and living systems and therefore plays a significant role in determining the fate and behaviour of the nanoparticles. This review summarises the state of the art in terms of understanding the bio-nano interface and provides direction for potential future research and recommendations for future priorities and strategies to support the safe implementation of nanotechnologies. The central premise is that nanomaterials must be studied as biological entities under the appropriate exposure conditions and that this should be implemented in study design and reporting for nanosafety assessment. The implications of the bio-nano interface for nanomaterials fate and behaviour are described in light of four interlinked perspectives: the Coating concept; the Translocation concept; the Signalling concept, and the Kinetics concept. A key conclusion is that nanoparticles cannot be viewed as non-interacting species, but rather must be thought of, and studied as, biological entities, where their interaction with the environment is mediated by the proteins and other biomolecules that adsorb to them, and the key parameter to characterise then becomes the nature, composition and evolution of the bionano interface.

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Optical probing and imaging of live cells using SERS labels

Cited by 146 publications

References 38 publications

Raman spectroscopy: techniques and applications in the life sciences

Raman spectroscopy: techniques and applications in the life sciences

Double Amino Functionalized Ag Nanoparticles as SERS Tags in Raman Diagnostic

The bio-nano-interface in predicting nanoparticle fate and behaviour in living organisms: towards grouping and categorising nanomaterials and ensuring nanosafety by design

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