Optical particle detection in liquid suspensions with a hybrid integrated microsystem

Bernat, I.; Gonzalez-Murillo, J.J.; Fonseca, L.; Moreno, Mauricio A.; Romano‐Rodríguez, A.

doi:10.1016/j.sna.2016.06.003

Cited by 5 publications

(2 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…High accuracy, as quantified by the percentage of the coefficient of variation of the measured signal (CV%), requires precise optical alignment of the detection region and the particle trajectory. To this end, inscribed optical waveguides, [14,26] micro-optics, [7,[27][28][29][30][31][32] and optical fibers [17,[19][20][21]33,34] are implemented in MFC platforms. Inscribed waveguides require extra coupling efforts to external lasers and detectors, thus suffering from low coupling efficiency and high-alignment dependency.…”

Section: Introductionmentioning

confidence: 99%

Viscoelastic Capillary Flow Cytometry

Serhatlıoğlu

Jensen

Niora

et al. 2022

Advanced NanoBiomed Research

View full text Add to dashboard Cite

A compact microfluidic flow cytometer is demonstrated, comprising viscoelastic flow focusing in fused silica capillaries and a fiber optical interface. Viscoelastic flow focusing enables simple device design and operation with a single‐inlet/outlet fluidic configuration. Fused silica capillaries with different inner diameters are effortlessly interchanged to eliminate blockage ratio limitations and enable single‐train particle focusing for a wide range of particle sizes and geometries. The compact system is mounted on an inverted microscope for easy integration with optical imaging and other optofluidic modalities, such as optical trapping and particle sorting. A real‐time cytometric analysis of three channels, forward scattering, side scattering, and fluorescence detection, is performed on LABVIEW. A throughput of 3500 events s−1 is performed on particles of sizes ranging from 2 to 20 μm, using capillaries of different inner diameters ranging from 30 to 75 μm. The outer diameter of all capillaries is identical to the cladding diameter of the applied optical fibers. This enables easy exchange and precise optical alignment of fibers and capillaries on a microfabricated jig. The performance of the microfluidic flow cytometer is benchmarked using polystyrene calibration beads, poly(lactic‐co‐glycolic acid) particles, erythrocytes, THP‐1 leukemic monocytes, and human metaphase chromosomes.

show abstract

Section: Introductionmentioning

confidence: 99%

Viscoelastic Capillary Flow Cytometry

Serhatlıoğlu

Jensen

Niora

et al. 2022

Advanced NanoBiomed Research

View full text Add to dashboard Cite

show abstract

“…The μTAS (Micro total analyzing system) with integrated microfluidic channels and fluid control units allow a system that is of comparable size to the particles of interest [20,21]. Microfluidic chips enable rapid detection of bacteria using various other techniques like optical [22,23], electrochemical [24,25], surface plasmon resonance (SPR) [26], quartz crystal microbalance (QCM) [27] and cantilever sensors [28]. Optical detection techniques are the most similar ones among the other techniques with regards to their detection principle.…”

Section: Introductionmentioning

confidence: 99%