On-chip classification of micro-particles using laser light scattering and machine learning

Hussain, Mubashir; Liu, Xiaolong; Zou, Jun; Yang, Jian; Ali, Zeeshan; Rehman, Hamood Ur; He, Nongyue; Dai, Jianrong; Tang, Yongjun

doi:10.1016/j.cclet.2021.09.044

Cited by 22 publications

(8 citation statements)

References 19 publications

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“…(3) Incorporating advanced techniques like 4D printing and machine learning to address challenges in ECM application. These techniques enable rapid screening of effective ECM material preparation, prediction of cellular behavior, and fabrication of smart transplantable tissue constucts. , (4) Developing real-time and noninvasive detective/visualization techniques to monitor tissue response to implanted ECM materials and the subsequent tissue regeneration process. Biomarkers or probes can play a crucial role in facilitating the fast iteration of material development and function validation. , To this end, the design of dECM-based constructs using biocompatible and biofunctional ECM materials, combined with advanced machine learning and fabrication techniques, holds promise for achieving the regenerative potential of dECM.…”

Section: Resultsmentioning

confidence: 99%

“…These techniques enable rapid screening of effective ECM material preparation, prediction of cellular behavior, and fabrication of smart transplantable tissue constucts. 72,73 (4) Developing real-time and noninvasive detective/visualization techniques to monitor tissue response to implanted ECM materials and the subsequent tissue regeneration process. Biomarkers or probes can play a crucial role in facilitating the fast iteration of material development and function validation.…”

Section: 7mentioning

confidence: 99%

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Cartilage Decellularized Extracellular Matrix-Based Hydrogel with Enhanced Tissue Adhesion and Promoted Chondrogenesis for Cartilage Tissue Engineering

Li,

Jiang,

Zhang

et al. 2024

ACS Appl. Polym. Mater.

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Articular cartilage defects caused by trauma or osteoarthritis remain a significant challenge in clinical practice due to their poor self-healing capacity. Current clinical approaches are generally limited by their complex procedures or certain postoperation complications. Therefore, it is crucial to develop an effective strategy for one-step cartilage repair with long-term functional cartilage regeneration. This study reports the creation of an injectable hydrogel composed of cartilage decellularized extracellular matrix (CdECM) and hyaluronic acid methacrylate (HAMA). In the presence of photoinitiator ruthenium (Ru)/sodium persulfate (SPS), visible light (450 nm) not only triggered the polymerization of CdECM and HAMA to form stable hydrogel in 1 min but also facilitated the tyrosine cross-links between biotissue and CdECM without any further moiety grafting, which cannot be achieved by routinely used photoinitiators and provides the hybrid hydrogel with bioadhesive properties. This dual-network hybrid hydrogel demonstrated robust adhesion strength to cartilage and significantly enhanced mechanical performance in comparison to that of CdECM or HAMA alone. Further in vitro culture demonstrated the promotive effect of hybrid hydrogel on encapsulated porcine bone marrow mesenchymal stem cells toward chondrogenic differentiation after 21 days of incubation compared with HAMA. Through in vivo subcutaneous implantation in a mouse model for two and 4 weeks, we further illustrated that the hybrid hydrogel has improved biocompatibility in comparison to HAMA alone. Thus, the presented hydrogel is a promising biomaterial for cartilage regeneration through minimally invasive arthroscopic injection in clinical practice.

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Section: Resultsmentioning

confidence: 99%

Section: 7mentioning

confidence: 99%

Cartilage Decellularized Extracellular Matrix-Based Hydrogel with Enhanced Tissue Adhesion and Promoted Chondrogenesis for Cartilage Tissue Engineering

Li,

Jiang,

Zhang

et al. 2024

ACS Appl. Polym. Mater.

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“…Next, the cells were incubated in a solution containing 6 µ m Mag‐Fluo‐4 AM (Thermo Fisher Scientific) and 2% pluronic acid (v/v; Thermo Fisher Scientific) in the dark at 37 °C for 1 h. Excess dye was washed off using Hank's Buffered Saline Solution, and fluorescenceintensity in live cells was measured using a fluorescent spectrophotometer at a wavelength of 495ex/515em (Molecular Devices, Gemini EM). [ 42 ] To assess cytosolic Ca 2+ levels, a high‐affinity ratiometric Ca 2+ dye, Fura‐2 AM (Thermo Fisher Scientific), was used as described previously. [ 43 ] Initially, cells were seeded in clear‐bottom white 96‐well plates (Nunc, Denmark) until reaching 80% confluence.…”

Section: Methodsmentioning

confidence: 99%

LRRK2 Gly2019Ser Mutation Promotes ER Stress via Interacting with THBS1/TGF‐β1 in Parkinson's Disease

Yao,

Lu,

Koc

et al. 2023

Advanced Science

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The gene mutations of LRRK2, which encodes leucine‐rich repeat kinase 2 (LRRK2), are associated with one of the most prevalent monogenic forms of Parkinson's disease (PD). However, the potential effectors of the Gly2019Ser (G2019S) mutation remain unknown. In this study, the authors investigate the effects of LRRK2 G2019S on endoplasmic reticulum (ER) stress in induced pluripotent stem cell (iPSC)‐induced dopamine neurons and explore potential therapeutic targets in mice model. These findings demonstrate that LRRK2 G2019S significantly promotes ER stress in neurons and mice. Interestingly, inhibiting LRRK2 activity can ameliorate ER stress induced by the mutation. Moreover, LRRK2 mutation can induce ER stress by directly interacting with thrombospondin‐1/transforming growth factor beta1 (THBS1/TGF‐β1). Inhibition of LRRK2 kinase activity can effectively suppress ER stress and the expression of THBS1/TGF‐β1. Knocking down THBS1 can rescue ER stress by interacting with TGF‐β1 and behavior burden caused by the LRRK2 mutation, while suppression of TGF‐β1 has a similar effect. Overall, it is demonstrated that the LRRK2 mutation promotes ER stress by directly interacting with THBS1/TGF‐β1, leading to neural death in PD. These findings provide valuable insights into the pathogenesis of PD, highlighting potential diagnostic markers and therapeutic targets.

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“…In recent years, signicant attention has been paid to colorimetric sensors based on precious metal nanoparticles. [17][18][19][20][21] These sensors have gained popularity due to their affordability, ease of use, and ability to observe samples with the naked eye. Among the noble metal nanoparticles, silver and gold nanoparticles are particularly favored because of their simplicity in preparation, precise size control, and strong surface plasmon resonance effect.…”

Section: Introductionmentioning

confidence: 99%

Anti-aggregation colorimetric sensing of cysteine using silver nanoparticles in the presence of Pb²⁺

Mao,

Zhang,

Zhang

et al. 2024

Anal. Methods

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On-chip classification of micro-particles using laser light scattering and machine learning

Cited by 22 publications

References 19 publications

Cartilage Decellularized Extracellular Matrix-Based Hydrogel with Enhanced Tissue Adhesion and Promoted Chondrogenesis for Cartilage Tissue Engineering

Cartilage Decellularized Extracellular Matrix-Based Hydrogel with Enhanced Tissue Adhesion and Promoted Chondrogenesis for Cartilage Tissue Engineering

LRRK2 Gly2019Ser Mutation Promotes ER Stress via Interacting with THBS1/TGF‐β1 in Parkinson's Disease

Anti-aggregation colorimetric sensing of cysteine using silver nanoparticles in the presence of Pb²⁺

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On-chip classification of micro-particles using laser light scattering and machine learning

Cited by 22 publications

References 19 publications

Cartilage Decellularized Extracellular Matrix-Based Hydrogel with Enhanced Tissue Adhesion and Promoted Chondrogenesis for Cartilage Tissue Engineering

Cartilage Decellularized Extracellular Matrix-Based Hydrogel with Enhanced Tissue Adhesion and Promoted Chondrogenesis for Cartilage Tissue Engineering

LRRK2 Gly2019Ser Mutation Promotes ER Stress via Interacting with THBS1/TGF‐β1 in Parkinson's Disease

Anti-aggregation colorimetric sensing of cysteine using silver nanoparticles in the presence of Pb2+

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Anti-aggregation colorimetric sensing of cysteine using silver nanoparticles in the presence of Pb²⁺