Opposite effects of Ca2+ on toxicity by CdCl2 on white blood cells (WBC), protein level and LD50 of rabbits Oryctolagus cuniculus

Abstract

Heavy metals and their derivatives are a special class of toxic substances. Many metals are useful for industrial, agricultural and medical applications. Indeed, they can go back through the food chain and thus achievable to human being. Our aim is to study an example of heavy metals cadmium (CdCl 2) at two concentrations 30 and 60 ppm on biological model Oryctolagus cuniculus that is often used in toxicological studies, and the possible neutralization using the Ca 2+. We are interested firstly of estimating LD 50 of CdCl 2 that is in range of 70 to 150 mg/kg according DVGW and we found that the LD 50 is 85.703 mg/kg of CdCl 2 , this valor is augmented with the addition of 30 ppm Ca 2+ to 108.231 mg/kg which show the protective role of Ca 2+. Our results also showed a significant increase in the protein level in treated rabbits with both doses, this augmentation is corrected with the addition of Ca 2+ at all days (7 th , 16 th and 21 st). Impact of CdCl 2 on total white blood cells (WBC) number showed a decrease in treatments with 30 ppm contrarily to the rabbits treated with 60 ppm where we found an increase; these perturbations are more or less corrected with Ca 2+ .