Oogram studies in mice infected with Schistosoma mansoni and treated with dexamethasone

Hermeto, Marco Victor; Bicalho, Rosilene Siray; Silva, Roney Pignaton da; Melo, Alan Lane de; Pereira, Leógenes Horácio

doi:10.1590/s0036-46651994000200001

Cited by 16 publications

(16 citation statements)

References 13 publications

(16 reference statements)

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“…Qualitative and quantitative oogram evaluation showed that arctiin did not affect oviposition kinetics or egg maturation. Similar effects on oogram were described with treatment of infected animals with dexamethasone (50 mg/kg) [34]. In addition, Aires et al [35] observed eggs in all development phases following administration of β-lapachone, however the percentage of immature eggs was significant lower, indicating a kinetic change in the egg development.…”

Section: Discussionsupporting

confidence: 56%

In vivo and In vitro of Arctiin Schistosomicidal Activity

Lc¹,

Mm²,

Pm³

et al. 2017

Clin Exp Pharmacol

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Human schistosomiasis, caused by trematode worms of the genus Schistosoma, is one of the most significant neglected tropical diseases, affecting more 200 million individuals worldwide and praziquantel is the only available drug to treat this neglected disease. Arctiin is a lignan obtained from Arctium lappa (Asteraceae) with antiinflammatory and antiproliferative activities. Our purpose was to investigate the in vitro and in vivo schistosomicidal activities of arctiin in mice infected with S. mansoni. Arctiin (200 and 100 μM) caused mortality, tegumental alterations, and reduction of motor activity of adult worms of S. mansoni in culture. Oral administration of a single dose of arctiin (25 mg/kg) on day 45 of infection did not reduce worm burden or cause any alteration in the analyzed parameters when compared to infected untreated mice. On the other hand, intraperitoneal treatment with arctiin (50 mg/kg) was able to reduce the hepatic granuloma volume by 20% in comparison to infected untreated mice. In addition, after intraperitoneal administration of arctiin in mice it was shown by HPLC analysis that arctiin was present in murine plasma. More studies should be conducted to verify the possible mechanism of action on inflammatory components present in granuloma formation.

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“…9 , 21, 44, 45 Our data are similar to results from other studies showing that glucocorticoids dampen the immune response to helminthic infection. 36,[46][47][48][49] We show that Dexa increased the time of adult female parasites remaining in the gastrointestinal tract, and the worms were fertile with release of eggs that were quantified in the feces. Dexa also led to increase in the burden of infective larvae recovered from the lungs of infected mice.…”

Section: Discussionmentioning

confidence: 81%

Dexamethasone Effects in the Strongyloides venezuelensis Infection in A Murine Model

Machado

Carlos²,

Sorgi³

et al. 2011

The American Journal of Tropical Medicine and Hygiene

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“…For the evaluation of quantitative oograms, the formula 'number of eggs/g of tissue ¼ number of eggs in intestinal fragment/weight of fragment in mg £ 1000' was employed (Cançado et al, 1965;Katz et al, 1966;Katz & Pellegrino, 1974;Hermeto et al, 1994). Additionally, in order to verify the number of eggs per female parasite in a day, the ratio of second-stage S. mansoni eggs per g intestine/number of female parasites recovered from the portal system was utilized (Pacheco et al, 2005(Pacheco et al, , 2008Mati et al, 2010).…”

Section: Oogram Proceduresmentioning

confidence: 99%

Current applications of oogram methodology in experimental schistosomiasis; fecundity of femaleSchistosoma mansoniand egg release in the intestine of AKR/J mice following immunomodulatory treatment with pentoxifylline

Mati

Melo

2012

J. Helminthol.

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AbstractOogram methodology permitted a direct assessment of the fecundity ofSchistosoma mansoniand the passage of parasite eggs from mesenteric vessels into the intestinal lumen in AKR/J mice that had been infected via the intraperitoneal route with 60 cercariae of the trematode and later subjected to short-term subcutaneous treatments with pentoxifylline (PTX). The administration of PTX did not change oviposition kinetics, the individual fecundity of female parasites (as determined by the study of the ratio of second-stageS. mansonieggs per g intestine/number of female parasites recovered from the portal system), nor the number of mature and dead eggs retained in the host tissue, though the drug has known immunomodulatory effects, as shown previously in experimental schistosomiasis. A better appraisal was also carried out, including the study of statistical parameters, concerning the utilization of the ratios of each stage of immature eggs (first to fourth) per g intestine/number of female worms from the portal system. The second-stage eggs had the lowest variability, confirming that the utilization of this stage as an indicator of the individual fecundity of parasite females is indeed viable. In the light of our findings, current uses of oogram methodology are discussed. Moreover, additional consideration is given to data obtained in the present study concerning intraperitoneal infection withS. mansonicercariae in both untreated and treated mice of the AKR/J strain, such as the recovery of mature worms, eggs and free granulomas from the peritoneal cavity of these rodents.

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“…Results from an oogram study in immunosuppressed mice performed by Hermeto et al (1994), showed a higher total amount of eggs and a higher amount of dead eggs in the intestine. No alterations in the percentage of the different immature viable eggs were found, and the amount of mature eggs was even higher in the immunosuppressed mice.…”

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confidence: 99%

Lower faecal egg excretion in chemically-induced diabetic mice infected with Schistosoma mansoni due to impaired egg maturation

Hulstijn

Oliveira

Moura

et al. 2001

Mem. Inst. Oswaldo Cruz

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(Turchetti-Maia et al. 1983), and insulin-dependent diabetes mellitus (Mahmoud et al. 1975) have been described to affect schistosomiasis in laboratory animals. Insulin-dependent diabetes mellitus (IDDM) is a metabolic disease, which is caused by the absence or the impaired functionallity of insulin and is mainly characterized by high blood glucose concentrations. IDDM can be induced in laboratory animals by injection of chemicals, like streptozotocin (STZ) (Rerup & Tarding 1969), which destroy the β-cells in the pancreas.Diabetes mellitus is associated with some defects in the cell-mediated immunity (Mandel & Mahmoud 1978) and individuals who suffer from this disease seem to be more susceptible to bacterial and fungal infections (Hart et al. 1969, Robertson & Polk 1974. In several experiments performed by Mahmoud et al. (1975Mahmoud et al. ( , 1976 and by Mahmoud (1979) netic diabetic mice were infected with Schistosoma mansoni. The results from these experiments showed that there was no difference between control and diabetic littermates in egg output, length and number of worms or in cercariae penetration through the skin. However, the size of the granuloma around the eggs, in both the liver and the lungs, was described to be smaller in diabetic mice. No difference was observed in granuloma formation around foreign bodies (divinyl benzene copolymer beads). These works show that IDDM affects the cell-mediated immune response around S. mansoni eggs in mice. On the other hand eosinophils favour the passage of schistosomal eggs to the intestinal lumen (Lenzi et al. 1987) and mice deprived of their T-cells have fewer eggs in their faeces (Doenhoff et al. 1978). Given that faecal egg excretion depends on the host immune response the present work was initiated to analise whether streptozotocin-induced diabetes mellitus could affect the faecal egg excretion.Swiss Webster mice (5 days old) were infected transcutaneously by exposing them to 50 cercariae (BH strain) of S. mansoni. From 40 days after the infection on, faeces was collected twice a week from each mouse individually. The faeces were processed according to the Kato-Katz method (Katz et al. 1972) and two slides per animal were counted. Forty-seven days after infection, IDDM was induced in 20 mice with an average weight of 33.9 ± 1.3 g by injecting STZ (180 mg per kg bodyweight, ip.) dissolved in citrate buffer (pH 4.5). Control

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“…Previous studies of the effects of corticoids on murine schistosomiasis showed variable effects that depended on the type of corticoid administered, the dose and the schedule of treatment (Harrison & Doenhoff 1983, Morrison et al 1986, Hermeto et al 1990). It was proposed that the decrease in worm burden that occurred following corticoid treatment was due to impairment of the initial phase of parasite penetration into host tissues (Hermeto et al 1994). Dexamethasone also decreased the level of collagen synthesis and the levels of enzymes associated with collagen synthesis (Newman & Cutroneo 1978, James et al 1983.…”

mentioning

confidence: 99%

Immunological and parasitological parameters after treatment with dexamethasone in murine Schistosoma mansoni

Aly

Hendawy

Ali

et al. 2010

Mem. Inst. Oswaldo Cruz

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Oogram studies in mice infected with Schistosoma mansoni and treated with dexamethasone

Cited by 16 publications

References 13 publications

In vivo and In vitro of Arctiin Schistosomicidal Activity

Dexamethasone Effects in the Strongyloides venezuelensis Infection in A Murine Model

Current applications of oogram methodology in experimental schistosomiasis; fecundity of femaleSchistosoma mansoniand egg release in the intestine of AKR/J mice following immunomodulatory treatment with pentoxifylline

Lower faecal egg excretion in chemically-induced diabetic mice infected with Schistosoma mansoni due to impaired egg maturation

Immunological and parasitological parameters after treatment with dexamethasone in murine Schistosoma mansoni

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