Oocyte in-vitro maturation: BCL2 mRNA content in cumulus cells reflects oocyte competency

Filali, Meryem; Frydman, Nelly; Belot, MP; Hesters, Laëtitia; Gaudin, Françoise; Tachdjian, Gérard; Émilie, Dominique; Frydman, René; Machelon, Véronique

doi:10.1016/s1472-6483(10)61071-1

Cited by 35 publications

(24 citation statements)

References 37 publications

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“…However, the expression kinetics of GREM1 and GLUT1 during IVM has not yet been well established. The up-regulation of BCL2 mRNA and absence of variation of BAX mRNA observed in cumulus cells of Control after IVM for 18 h are in accordance with that reported by Filali et al (2009) in human COCs. According to these authors, the ratio BCL2 (anti-apoptotic)/BAX (pro-apoptotic) determines cell survival, and the prevalence of BCL2 mRNA expression in cumulus cells is directly related to oocyte competence.…”

Section: Cumulus Expansion Degreesupporting

confidence: 90%

Gene expression in sheep cumulus-oocyte complexes meiotically inhibited with roscovitine

Crocomo

Filho

Ackermann

et al. 2015

Small Ruminant Research

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The majority of mammalian oocytes destined for in vitro maturation (IVM) have not undergone all molecular and structural changes necessary for competence acquisition to support the fertilization and early embryogenesis. In this context, different methods able to provide a transient arrest of meiosis resumption have been tested in order to improve the in vitro developmental potential of oocytes. Based on that, our study aimed to evaluate the effect of temporary meiosis inhibition using roscovitine on gene expression in sheep oocytes and cumulus cells. For this, cumulus-oocyte complexes (COCs) were cultured for 6 h in modified TCM199 medium with (Rosco) and without (Control) 75 M roscovitine. Subsequently, they were in vitro matured for a further 18 h in inhibitor-free TCM199 medium supplemented with gonadotropins. At 0, 6 and 24 h of culture, nuclear status of oocytes and expression of selected genes were evaluated by Hoescht staining and qRT-PCR, respectively. The analysis of oocyte chromatin organization revealed that roscovitine efficiently inhibited the meiosis of sheep oocytes for 6 h and its action was completely reversed after 18 h of in vitro maturation in inhibitor-free medium. Besides, no detrimental effect on cumulus expansion was observed. The expression profile of most investigated genes in cumulus cells (PTX3, GREM1, GLUT1, PTGS2, ALK5, ALK6) and oocytes (ZAR1, NLRP5, SOD1, BMP15, GDF9) was similar between Control and Rosco treatments and the ratio BCL2/BAX was maintained in both cell types even in the presence of roscovitine. These results indicate that reversible meiotic arrest promoted by roscovitine, at the concentration and exposure time studied, neither impaired nor improved the expression of investigated genes in sheep oocytes and cumulus cells. Moreover, the efficiency of temporary meiotic arrest and the absence of deleterious effect on COCs suggest that roscovitine provides a useful method for transportation or manipulation of sheep oocytes at onset of maturation. However, further investigations are necessary to evaluate the benefits of roscovitine treatment on in vitro development of sheep embryos and its effects on cellular ultrastructure.

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Section: Cumulus Expansion Degreesupporting

confidence: 90%

Gene expression in sheep cumulus-oocyte complexes meiotically inhibited with roscovitine

Crocomo

Filho

Ackermann

et al. 2015

Small Ruminant Research

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“…2 According to a study by Filali et al (2009), women with FSH and LH therapy showed increasing Bcl-2 expression and no apoptosis in ovaries. 7 Differences in Bcl-2 expression are also caused by increasing ROS. Cypermethrin can induce oxidative stress, which will produce an imbalance of oxidants and antioxidants.…”

Section: Based On Data Analysis From Pearson Correlation Test Betweenmentioning

confidence: 99%

Effects of per oral cypermethrin exposure on Bcl-2 expression in granulose cells and antral follicle count of Rattus norvegicus ovaries

Indrayanti

Rahardjo

Sujuti

2019

MOG

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ABSTRAKObjectives: To determine the effect of oral cypermethrin exposure on the decrease of Bcl-2 expression in granulosa cells and antral follicle count in Rattus norvegicus ovary. Materials and Methods:This was a true experimental study using post-test control group design. This study used 24 Rattus norvegicus which were divided into 4 groups, the control group, treatment group (P1) exposed to cypermethrin 10 mg/kg BW, treatment group (P2) exposed to cypermethrin 15 mg/kg BW and treatment group (P3) exposed to cypermethrin 20 mg/kg BW. The treatment groups were exposed to cypermethrin for 28 days. A surgery was conducted in proestrus phase and ovarian organs were taken. Bcl-2 expression examination was performed by using immunohistochemical method and the antral follicle count was assessed with the Hematoxillin Eosin (HE) Results: This study showed the decrease of Bcl-2 expression in cypermethrin-exposed group compared to control group. There was a decrease in antral follicles count in cypermethrin-exposed group compared to control group. Conclusion: Cypermethrin can decrease Bcl-2 expression and antral follicles count in Rattus norvegicus ovaries.

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“…BCL2 works as an anti-apoptotic gene to promote cell survival, whereas BCL2-associated X protein (BAX) acts as a pro-apoptotic gene to induce cell death [Zhang et al 2013]. The ratio of BCL2 to BAX could be used to predict the fate of cumulus oocyte complexes (COCs) after various treatments [Oltvai et al 1993;Gursoy et al 2008] and it could also be associated with the ability of oocytes to complete nuclear maturation [Filali et al 2009]. Hyaluronan synthase 2 (HAS2), prostaglandin endoperoxide synthase 2 (PTGS2), and steroidogenic acute regulatory protein (STAR) genes expressed especially in cumulus cells during in vitro maturation that have been associated with developmental competence of oocytes and were suggested as predictors of embryo quality in women and cow [Assidi et al 2008;Calder et al 2001;Fair and Lonergan 2012;Nuttinck et al 2002].…”

Section: Introductionmentioning

confidence: 99%

Impact of supplementary royal jelly onin vitromaturation of sheep oocytes: genes involved in apoptosis and embryonic development

Amiri

Deldar

Pirsaraei

2015

Systems Biology in Reproductive Medicine

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Optimizing culture conditions lead to the improvement of oocyte developmental competence and additives with anti-oxidative activity in culture media improved embryonic development. Royal jelly (RJ) is a product from the cephalic glands of nurse bees that has considerable health effects. The aim of this study was to investigate the effect of different concentrations of RJ on the maturation, cleavage, and blastocyst rates and gene expression in the oocyte and cumulus cells during in vitro maturation (IVM) of sheep oocyte. IVM of oocyte was performed in the presence of control (RJ0), 2.5 (RJ2.5), 5 (RJ5), 10 (RJ10), 20 (RJ20), and 40 (RJ40) mg/mL of RJ. Following the maturation period, parthenogenetic activation was carried out in two treatment groups (RJ0 and RJ10) and embryonic development was examined three and eight days thereafter. Moreover, the relative expression of BCL2 and BAX in oocyte as well as BCL2, BAX, HAS2, PTGS2, and STAR in cumulus cells were assessed. The results indicated that the addition of 10 mg/mL of RJ (90 ± 4.51%) to the maturation medium linearly increased the oocyte maturation rate compared to the control group (57 ± 2.42%), then it remained constant to the RJ40 (93 ± 3.10%) group. The higher RJ concentrations were associated with increased (p < 0.01) cleavage (53.3 ± 1.55% to 82.3 ± 2.82%) and blastocyst rate (15.5 ± 1.16% to 33.8 ± 3.09%) from the RJ0 to the RJ10 group. The relative mRNA expression of BCL2 and BAX in the oocyte was higher at RJ10. In cumulus cells, the expression of BCL2 was not affected, but that of BAX decreased, and expression of HAS2, PTGS2, and STAR were increased following the addition of RJ to the maturation media. In conclusion, the addition of 10 mg/mL of RJ to maturation medium improved blastocyst formation and decreased the apoptotic incidence in sheep cumulus cells and the oocyte during the in vitro development.

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Oocyte in-vitro maturation: BCL2 mRNA content in cumulus cells reflects oocyte competency

Cited by 35 publications

References 37 publications

Gene expression in sheep cumulus-oocyte complexes meiotically inhibited with roscovitine

Gene expression in sheep cumulus-oocyte complexes meiotically inhibited with roscovitine

Effects of per oral cypermethrin exposure on Bcl-2 expression in granulose cells and antral follicle count of Rattus norvegicus ovaries

Impact of supplementary royal jelly onin vitromaturation of sheep oocytes: genes involved in apoptosis and embryonic development

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